Dual‐ and single‐shot susceptibility ratio measurements with circular polarizations in second‐harmonic generation microscopy

Golaraei, Ahmad; Kontenis, Lukas; Karunendiran, Abiramy; Stewart, Bryan A.; Barzda, Virginijus

doi:10.1002/jbio.201960167

Cited by 16 publications

(27 citation statements)

References 57 publications

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“…, where the superscripts refer to the PSG polarization states, and the subscripts identify Stokes vector components of SHG signal. The R-ratio was calculated in terms of 1st and 4th elements of the measured SHG Stokes vector [48]:…”

Section: Polarimetric Parameter Calculationsmentioning

confidence: 99%

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Machine learning-enabled cancer diagnostics with widefield polarimetric second-harmonic generation microscopy

Mirsanaye

Kamaliddin

et al. 2021

Preprint

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The extracellular matrix (ECM) collagen undergoes major remodeling during tumorigenesis. However, alterations to the ECM are not widely considered in cancer diagnostics, due to mostly uniform appearance of collagen fibers in white light images of hematoxylin and eosin-stained tissue sections. Polarimetric second-harmonic generation (P-SHG) microscopy enables label-free visualization and ultrastructural investigation of non-centrosymmetric molecules, which, when combined with texture analysis, provides multiparameter characterization of tissue collagen. This paper demonstrates whole slide imaging of breast tissue microarrays using high-throughput widefield P-SHG microscopy. The resulting P-SHG parameters are used in classification to differentiate tumor tissue from normal with 94.2% accuracy and F1-score, and 6.3% false discovery rate. Subsequently, the trained classifier is employed to predict tumor tissue with 91.3% accuracy, 90.7% F1-score, and 13.8% false omission rate. As such, we show that widefield P-SHG microscopy reveals collagen ultrastructure over large tissue regions and can be utilized as a sensitive biomarker for cancer diagnostics and prognostics studies.

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Section: Polarimetric Parameter Calculationsmentioning

confidence: 99%

“…In addition, we computed SHG-CD, which was conventionally expressed as [46,47,48]…”

Section: Polarimetric Parameter Calculationsmentioning

confidence: 99%

Machine learning-enabled cancer diagnostics with widefield polarimetric second-harmonic generation microscopy

Mirsanaye

Kamaliddin

et al. 2021

Preprint

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“…Most often hematoxylin and eosin (H&E) stained histology slides are used as a gold standard for cancer diagnostics and are typically imaged with white-light or fluorescence image contrasts [8]. Multicontrast nonlinear optical microscopy with multiphoton excitation fluorescence (MPF), second harmonic generation (SHG) and third harmonic generation (THG) can also be employed for histopathology investigations [9][10][11][12][13][14][15][16][17][18][19]. Imaging the same slides with nonlinear microscopy presents a convenient way of gathering more information about the tissue structure without altering the H&E slide preparation procedure.…”

Section: Introductionmentioning

confidence: 99%

Wide-Field Multicontrast Nonlinear Microscopy for Histopathology

Castaño

Mirsanaye

Golaraei

et al. 2022

Preprint

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A multicontrast polarimetric wide-field second harmonic generation (SHG) and multiphoton excitation fluorescence (MPF) microscope is optimized for large area imaging of hematoxylin and eosin (H&E) stained and unstained histology slides. The bleaching kinetics of MPF and SHG are examined with various laser intensities at different pulse repetition rates to determine the optimal wide-filed imaging conditions for H&E stained histology slides. Several polarimetric parameters are used to investigate the organization of extracellular matrix collagen in the histology samples.

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“…Revealing the mechanisms of interaction between photons and complex biological tissues is one of the major goals of biomedical optics research, which is essential for therapeutic and diagnostic applications [1–9]. Polarization is one of the fundamental properties of light and a promising method that has been applied to many fields, including biological tissues [3, 10–14]. Most biological tissues are structurally anisotropic turbid media containing fibrous components, such as axons, capillary vessels, collagen fibers or myofibrils [15].…”

Section: Introductionmentioning

confidence: 99%

Study of the spatial scale stability of Mueller matrix parameters for textural characterization of biological tissues

Chen

Chu

Tang

et al. 2021

Journal of Biophotonics

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Mueller matrix imaging polarimetry (MMIP) is a promising technique for the textural characterization of biological tissue structures. To reveal the influence of imaging magnification on the robustness of Mueller matrix parameters (MMPs), the spatial scale stability of MMPs was studied. We established a new MMIP detector and derived the mathematical model of the spatial scale stability of MMPs. The biological tissues with well‐defined structural components were imaged under different magnifications. Then, we compared and analyzed the textural features of the MMPs in the resulting images. The experimental results match the predictions of the mathematical model in these aspects: (a) magnification exhibits a strong nonlinear effect on the textural contrasts of MMPs images; (b) higher magnification does not necessarily lead to superior contrast for textural characterization; and (c) for different biological tissues, MMPs contrasts can be optimized differently, with some showing superior results. This study provides a reference for the experimental design and operation of the MMIP technique and is helpful for improving the characterization ability of MMPs.

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Dual‐ and single‐shot susceptibility ratio measurements with circular polarizations in second‐harmonic generation microscopy

Abstract: Polarization-resolvedsecondharmonic generation (P-SHG)

Cited by 16 publications

References 57 publications

Machine learning-enabled cancer diagnostics with widefield polarimetric second-harmonic generation microscopy

Machine learning-enabled cancer diagnostics with widefield polarimetric second-harmonic generation microscopy

Wide-Field Multicontrast Nonlinear Microscopy for Histopathology

Study of the spatial scale stability of Mueller matrix parameters for textural characterization of biological tissues

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