Ultrastructural evaluation of human metaphase II oocytes after vitrification: closed versus open devices

Bonetti, Antonella; Cervi, Marta; Tomei, Francesco; Marchini, Maurizio; Ortolani, Fulvia; Manno, Massimo

doi:10.1016/j.fertnstert.2010.08.027

Cited by 64 publications

(43 citation statements)

References 54 publications

(59 reference statements)

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“…This level of survival has repeatedly been obtained [10,[47][48][49] but not by all groups (77%; [50], 81%; [51] and when used in conjunction with a closed system has resulted in a reduced survival (75%; [52]). Manipulation of the equilibration of the permeable cryoprotectant and water prior to vitrification in a closed system has resulted in a high rate of survival (95%) only previously observed in open systems but blastocyst development appeared to be reduced [40], possibly as a consequence of ultrastructural damage [53]. It has also been shown that a significant reduction in survival results following transport at −196°C of oocytes vitrified in the above open system [54].…”

Section: Discussionmentioning

confidence: 90%

Implantation rates of embryos generated from slow cooled human oocytes from young women are comparable to those of fresh and frozen embryos from the same age group

Gook

Edgar

2011

J Assist Reprod Genet

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Previous reports of slow cooling of human mature oocytes have shown a reduced clinical efficiency relative to fresh oocytes. This study reports that equivalent fertilization and implantation rates to those obtained using fresh oocytes and cryopreserved embryos can be achieved with human mature oocytes dehydrated in 1.5 M propanediol and 0.2 M sucrose at 37°C and cryopreserved using slow cooling rates.

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Section: Discussionmentioning

confidence: 90%

Implantation rates of embryos generated from slow cooled human oocytes from young women are comparable to those of fresh and frozen embryos from the same age group

Gook

Edgar

2011

J Assist Reprod Genet

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“…After full-text assessment, 9 articles were excluded. Therefore, 4 articles were finally included in the systematic review and meta-analysis [8-11]. …”

Section: Resultsmentioning

confidence: 99%

Survival Rates in Closed and Open Vitrification for Human Mature Oocyte Cryopreservation: A Meta-Analysis

Youm¹,

Choi²,

Oh³

et al. 2017

Gynecol Obstet Invest

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Background/Aims: Survival of warmed oocytes is the first part and is one of the most important aspects of in vitro fertilization following oocyte cryopreservation. There is no definite conclusion about the difference in survival rates between closed and open vitrification for oocyte cryopreservation. This study aimed to investigate whether there is a difference in survival rates between closed and open vitrification for cryopreservation of human mature oocytes. Methods: A literature search was performed using the MEDLINE, Cochrane Central Register of Controlled Trials, and Embase databases. A total of 4 studies were included in the meta-analysis. Results: In the frequentist meta-analysis, the random effects model showed no statistically significant difference in survival rates between closed and open vitrification (risk ratio [RR] 0.8873, 95% confidence interval [CI] 0.7658–1.0280). However, an effect size deviation of the RR from 1.0 and a borderline trend of the 95% CI were noted. In the Bayesian meta-analysis, the posterior probability that closed vitrification would decrease the survival rate when compared to that with open vitrification was 83.04% for the random effects model. Conclusion: It is not yet possible to conclude that closed vitrification is an aseptic alternative to open vitrification in human mature oocyte cryopreservation.

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“…The information available on the neonatal outcome and the long-term follow-up of children conceived after oocyte cryopreservation is limited (Chian et al, 2008(Chian et al, , 2014Noyes et al, 2009;Wennerholm et al, 2009;Cobo et al, 2014) and only small studies have specifically addressed the safety of oocyte vitrification (Coticchio et al, 2009;Bonetti et al, 2011;Chen et al, 2012;Forman et al, 2012;Khalili et al, 2012;Monzo et al, 2012;Dominguez et al, 2013;Gugliemo et al, 2014;Konc et al, 2014;Nohales Có rcoles et al, 2014;Palmerini et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Chromosomal meiotic segregation, embryonic developmental kinetics and DNA (hydroxy)methylation analysis consolidate the safety of human oocyte vitrification

Munck

Petrussa

Verheyen³

et al. 2015

MHR: Basic Science of Reproductive Medicine

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Oocyte vitrification has been introduced into clinical settings without extensive pre-clinical safety testing. In this study, we analysed major safetyaspects of human oocyte vitrification in a high security closed system: (i) chromosomal meiotic segregation, (ii) embryonic developmental kinetics and (iii) DNA (hydroxy)methylation status. Fresh and vitrified sibling oocytes from young donors after intracytoplasmic sperm injection (ICSI) were compared in three different assays. Firstly, the chromosomal constitution of the fertilized zygotes was deduced from array comparative genomic hybridization results obtained from both polar bodies biopsied at Day 1. Secondly, embryo development up to Day 3 was analysed by time-lapse imaging. Ten specific time points, six morphokinetic time intervals and the average cell number on Day 3 were recorded. Thirdly, global DNA methylation and hydroxymethylation patterns were analysed by immunostaining on Day 3 embryos. The nuclear fluorescence intensity was measured by Volocity imaging software. Comprehensive chromosomal screening of the polar bodies demonstrated that at least half of the zygotes obtained after ICSI of fresh and vitrified oocytes were euploid. Time-lapse analysis showed that there was no significant difference in cleavage timings, the predictive morphokinetic time intervals nor the average cell number between embryos developed from fresh and vitrified oocytes. Finally, global DNA (hydroxy)methylation patterns were not significantly different between Day 3 embryos obtained from fresh and from vitrified oocytes. Our data further consolidate the safety of the oocyte vitrification technique. Nevertheless, additional testing in young and older sub-fertile/infertile patients and sound follow-up studies of children born after oocyte cryopreservation remain mandatory.

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Ultrastructural evaluation of human metaphase II oocytes after vitrification: closed versus open devices

Cited by 64 publications

References 54 publications

Implantation rates of embryos generated from slow cooled human oocytes from young women are comparable to those of fresh and frozen embryos from the same age group

Implantation rates of embryos generated from slow cooled human oocytes from young women are comparable to those of fresh and frozen embryos from the same age group

Survival Rates in Closed and Open Vitrification for Human Mature Oocyte Cryopreservation: A Meta-Analysis

Chromosomal meiotic segregation, embryonic developmental kinetics and DNA (hydroxy)methylation analysis consolidate the safety of human oocyte vitrification

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