Survival Rates in Closed and Open Vitrification for Human Mature Oocyte Cryopreservation: A Meta-Analysis

Youm, Hyun Sik; Choi, Jong-Ryeol; Oh, Daesik; Rho, Yoomi

doi:10.1159/000484243

Cited by 11 publications

(7 citation statements)

References 14 publications

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“…In humans, in which the vitrification of in vivo matured oocytes is routinely performed in clinical practice, protocols make use of a two-step increase in the CPA concentrations [ 53 , 54 ]. In the first step, the oocytes are incubated for a relatively long time (10–15 min) in equilibration solution, and the second step consists of exposure to the vitrification solution for 30–90 s [ 55 , 56 , 57 ].…”

Section: Discussionmentioning

confidence: 99%

New Alternative Mixtures of Cryoprotectants for Equine Immature Oocyte Vitrification

Angel‐Velez

Coster

Azari‐Dolatabad

et al. 2021

Animals

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Equine oocyte vitrification would benefit the growing in vitro embryo production programs, but further optimization of the protocol is necessary to reach clinical efficiency. Therefore, we aimed to perform a direct comparison of non-permeating and permeating cryoprotective agents (CPAs) during the vitrification and warming of equine immature oocytes. In the first experiment, cumulus oocytes complexes (COCs) were vitrified comparing sucrose, trehalose, and galactose in combination with ethylene glycol (EG) and dimethyl sulfoxide (DMSO). In the second experiment, the COCs were vitrified using three mixtures of permeating CPAs in a 50:50 volume ratio (ethylene glycol-dimethyl sulfoxide (ED), propylene glycol-ethylene glycol (PE), and propylene glycol-dimethyl sulfoxide (PD)) with galactose and warmed in different galactose concentrations (0.3 or 0.5 mol/L). Overall, all the treatments supported blastocyst formation, but the developmental rates were lower for all the vitrified groups in the first (4.3 to 7.6%) and the second (3.5 to 9.4%) experiment compared to the control (26.5 and 34.2%, respectively; p < 0.01). In the first experiment, the maturation was not affected by vitrification. The sucrose exhibited lower cleavage than the control (p = 0.02). Although the galactose tended to have lower maturation than trehalose (p = 0.060) and control (p = 0.069), the highest numerical cleavage and blastocyst rates were obtained with this CPA. In the second experiment, the maturation, cleavage, and blastocyst rates were similar between the treatments. Compared to the control, only the ED reached similar maturation (p = 0.02) and PE similar cleavage (p = 0.1). The galactose concentration during warming did not affect the maturation, cleavage, or blastocyst rates (p > 0.1), but the PE-0.3 exhibited the highest blastocyst rate (15.1%) among the treatments, being the only one comparable to the control (34.2%). As such, PE–galactose provides a valuable option for equine immature oocyte vitrification and should be considered for the future optimization of the protocol.

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Section: Discussionmentioning

confidence: 99%

New Alternative Mixtures of Cryoprotectants for Equine Immature Oocyte Vitrification

Angel‐Velez

Coster

Azari‐Dolatabad

et al. 2021

Animals

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“…The use of 0.5 M nonpermeable CPA during warming is considered sufficient. To improve the survival rate of VW oocytes, many studies have attempted to stabilize the rehydration process during warming by increasing sucrose concentrations [ 27 , 42 ]. However, increasing the viscosity of the warming solution did not show any positive effects on the survival and embryo development of VW oocytes using the CC in this study ( Figures S1 and S2, Supplementary Materials ).…”

Section: Discussionmentioning

confidence: 99%

“…Live birth rates tend to be lower when vitrification of human blastocysts and oocytes is performed using CC compared to OC [ 20 , 26 ]. The survival rates of human mature oocytes after vitrification using CC were relatively lower than those of OC [ 27 ]. Therefore, further studies remain necessary to balance the efficiency and safety of oocyte vitrification using CC.…”

Section: Introductionmentioning

confidence: 99%

Development of Optimized Vitrification Procedures Using Closed Carrier System to Improve the Survival and Developmental Competence of Vitrified Mouse Oocytes

Park

Lim

et al. 2021

Cells

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The open carrier system (OC) is used for vitrification due to its high efficiency in preserving female fertility, but concerns remain that it bears possible risks of cross-contamination. Closed carrier systems (CC) could be an alternative to the OC to increase safety. However, the viability and developmental competence of vitrified/warmed (VW) oocytes using the CC were significantly lower than with OC. We aimed to improve the efficiency of the CC. Metaphase II oocytes were collected from mice after superovulation and subjected to in vitro fertilization after vitrification/warming. Increasing the cooling/warming rate and exposure time to cryoprotectants as key parameters for the CC effectively improved the survival rate and developmental competence of VW oocytes. When all the conditions that improved the outcomes were applied to the conventional CC, hereafter named the modified vitrification/warming procedure using CC (mVW-CC), the viability and developmental competence of VW oocytes were significantly improved as compared to those of VW oocytes in the CC. Furthermore, mVW-CC increased the spindle normality of VW oocytes, as well as the cell number of blastocysts developed from VW oocytes. Collectively, our mVW-CC optimized for mouse oocytes can be utilized for humans without concerns regarding possible cross-contamination during vitrification in the future.

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“…Recently, two systematic reviews reported the reproductive outcomes of human oocytes and embryos comparing open versus closed vitrification [ 28 , 29 ]. One study suggested that it was not yet possible to conclude that closed vitrification is an aseptic alternative to open vitrification in human mature oocyte cryopreservation.…”

Section: Discussionmentioning

confidence: 99%

“…One study suggested that it was not yet possible to conclude that closed vitrification is an aseptic alternative to open vitrification in human mature oocyte cryopreservation. In this study, only four articles concerning cyrosurvival rate after vitrification were included, of which the strength of evidence was low [ 29 ]. The other study regarding embryo vitrfication concluded with similar results on cryosurvival rates, implantation rates, clinical pregnancy rates and live birth rates, where seven articles were included.…”

Section: Discussionmentioning

confidence: 99%

Open versus closed vitrification system of human oocytes and embryos: a systematic review and meta-analysis of embryologic and clinical outcomes

Cai

Niringiyumukiza

et al. 2018

Reprod Biol Endocrinol

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BackgroundThe objective of this study was to carry out a systematic review and meta-analysis of embryologic and clinical outcomes following open versus closed vitrification of human oocytes and embryos.MethodsAn electronic literature search was conducted in main electronic databases up to June 30, 2018 using the following key terms: ‘oocyte’, ‘embryo’, ‘blastocyst’, ‘vitrification’, ‘cryopreservation’, ‘device’, ‘survival rate’, ‘pregnancy rate’, etc. A meta-analysis was performed using a random effect model to estimate the value of risk ratios (RRs) and 95% confidence interval (CI). Subgroup analyses and sensitivity analyses were carried out to further confirm the results.ResultsTwelve (Eight prospective and four retrospective) studies comparing open versus closed vitrification of human oocytes or embryos were included. For prospective studies on oocytes, no evidence for a significant difference in cryosurvival rate (RR = 0.91, 95% CI: 0.80–1.03, P = 0.14; n = 2048) or clinical pregnancy rate (RR = 1.29, 95% CI: 0.80–2.06, P = 0.30; n = 150) was observed. Additionally, there were no significant differences between the two methods concerning secondary endpoints included positive βHCG rate, implantation rate, miscarriage rate, ongoing pregnancy rate, live birth rate, cancellation rate, babies born per transferred blastocysts, or multiple birth rate (P > 0.05). The results of the retrospective studies were similar as the prospective studies.ConclusionsIt is still impossible to conclude that closed vitrification system could be a substitution for open system in human oocyte and embryo cryopreservation based on current evidence. Therefore, more well-designed prospective studies addressing these issues are still warranted.

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Survival Rates in Closed and Open Vitrification for Human Mature Oocyte Cryopreservation: A Meta-Analysis

Cited by 11 publications

References 14 publications

New Alternative Mixtures of Cryoprotectants for Equine Immature Oocyte Vitrification

New Alternative Mixtures of Cryoprotectants for Equine Immature Oocyte Vitrification

Development of Optimized Vitrification Procedures Using Closed Carrier System to Improve the Survival and Developmental Competence of Vitrified Mouse Oocytes

Open versus closed vitrification system of human oocytes and embryos: a systematic review and meta-analysis of embryologic and clinical outcomes

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