Ultrastructural characteristics of developingeosinophil leukocytes in human bone marrow during acute leukemia. Evidence for extracellular granule release from human eosinophils

Anteunis, A; Astesano, A.; Robineaux, R

doi:10.1007/bf00920871

Cited by 6 publications

(4 citation statements)

References 22 publications

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“…Although eosinophil infiltration was present in hematopoietic and lymphatic tissues, our studies of spleen, lymph nodes, and thymus showed scant evidence of eosinophil degranula- tion. While bone marrow was not analyzed in our immunofluorescence studies, several reports have shown evidence of degranulation by eosinophilic promyelocytes (Scott et al, 1970;Anteunis et al, 1977;Hyman et al, 1978), and Butterfield et al (1984) found evidence consistent with eosinophilic degranulation.…”

Section: Discussionmentioning

confidence: 87%

Eosinophil infiltration and degranulation in normal human tissue

et al. 1998

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Localization of eosinophil granule major basic protein by immunofluores-cence permits recognition of both eosinophil infiltration and degranulation. Over the past decade and a half, our laboratory has shown that eosinophil infiltration and degranulation occur in many diseased tissues in humans; among normal tissues studied as controls, only the gut showed striking eosinophil infiltration and degranulation. Using an indirect immunofluores-cence procedure for the detection of major basic protein, we extended our analyses of normal human tissues to include tissues from essentially all body organs; a total of 117 biopsy/autopsy specimens were analyzed. To determine whether the method of tissue procurement affected the level of eosinophil degranulation in the normal gastrointestinal tract, normal proximal jejunum from six patients was biopsied using either an endoscopic forceps or a scalpel at the time of elective surgery and examined by immunofluorescence. Spleen, lymph node, and thymus tissues showed eosinophil infiltration with scant evidence of degranulation, but the only organ showing both eosinophil infiltration and remarkable degranulation was the gastrointestinal tract. Eosinophil degranu-lation was significantly increased in specimens obtained by endoscopic forceps compared to those obtained by scalpel (P 0.021). These results indicate that tissue procurement methods affect the degree of eosinophil degranulation in the gut. Thus, among normal human body organs, both eosinophil infiltration and appreciable degranulation consistently occur only in the gut. Anat.

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Section: Discussionmentioning

confidence: 87%

Eosinophil infiltration and degranulation in normal human tissue

et al. 1998

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“…round, unicompartmental structure evident in the youngest eosinophils; the latter IG, also a large, unicompartmental structure, progressively condensed and crystallized to form large numbers of bicompartmental secondary (specific) granules which typify mature eosinophils. Other investigators in studies of immature human eosinophils have identified exocytosis of primary granules from eosinophils [2,5,23,[37][38][39][40], providing an explanation for the marked reduction in this granule population in mature cells.…”

Section: Primary Granules Are Unique Unicompartmental Clc-p-rich Ormentioning

confidence: 99%

“…Large amounts of extracellular CLC-P most likely originate from release of cytoplasmic and nuclear stores of CLC-P from damaged eosinophils. Secretion of primary granules from immature eosinophils in bone marrow samples by classical exocytosis has been recorded [2,5,[37][38][39][40] and probably accounts for the marked reduction in this granule population in mature cells.…”

Section: Secretion Release and Resolution Of Secreted/released Humamentioning

confidence: 99%

Ultrastructural Analysis of Human Eosinophils

Dvorak

Weller²

2000

Chemical Immunology and Allergy

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“…\nd more classical secretory events, such as granule e.xtrusion. have been documented in previous ultrastructurai studies [15][16][17][18][19]. Therefore, we re-examined these developmenially arrested eosinophilic myelocytes.…”

mentioning

confidence: 81%

Vesicular transport of peroxidase in human eosinophilic myelocytes

Dvorak¹,

Estrella²,

Ishizaka³

1994

Clin Experimental Allergy

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We performed ultrastructural cytochemistry to detect peroxidase in developmentally arrested human eosinophilic myelocytes. Human umbilical cord blood mononuclear cells were cultured for 21 days in the presence of murine-derived conditioned media, resulting in the development of eosinophilic myelocytes. Unlike normally developing eosinophilic myelocytes, which contain peroxidase in synthetic organelles (i.e. cisterns surrounding the nucleus and bounded by the rough endoplasmic reticulum and Golgi structures) and in immature and mature granules, the developmentally arrested cells showed ultrastructural evidence of decreased synthesis and secretory transport of peroxidase. Thus, peroxidase was generally absent in the perinuclear and rough endoplasmic cisterns, in Golgi structures, in immature granules and in the matrix compartment of most mature granules. Rather, biocompartmental specific granules displayed empty, peroxidase-negative matrix and central, peroxidase-negative core material. Peroxidase was present in perigranular vesicles, some of which were attached to granules. Such peroxidase-loaded transport vesicles are similar to those that effect piecemeal degranulation of mature human eosinophils cultured in rhIL-5-containing media [1]. These findings establish vesicle-mediated piecemeal degranulation in the secretory repertoire of immature human eosinophils and suggest the possibility that eosinophilic myelocytes may participate in vivo in important physiological and/or pathological events that require selective secretion from the specific granule matrix compartment.

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Ultrastructural characteristics of developingeosinophil leukocytes in human bone marrow during acute leukemia. Evidence for extracellular granule release from human eosinophils

Cited by 6 publications

References 22 publications

Eosinophil infiltration and degranulation in normal human tissue

Eosinophil infiltration and degranulation in normal human tissue

Ultrastructural Analysis of Human Eosinophils

Vesicular transport of peroxidase in human eosinophilic myelocytes

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