Ultrastructural changes in the sublingual salivary gland of prenatal buffalo (Bubalus bubalis)

Singh, Amandeep; Singh, Opinder

doi:10.14202/vetworld.2016.326-329

Cited by 5 publications

(7 citation statements)

References 5 publications

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“…A presence of LDH was also noticed in the tear and the fluid of lacrimal gland of rat and rabbit ( Thorig, Van Haeringen & Wijngaards, 1984 ). Similarly, a moderate to strong reaction was also reported in the acini of lacrimal gland in neonatal buffalo calves ( Singh, Malhi & Singh, 1999 ). The involvement and high activity of G-6-PD was also noticed during corneal epithelial growth and cell differentiation increased up to 150-fold when corneal epithelial cells constituted a differentiated four- to five-layered epithelium.…”

Section: Discussionmentioning

confidence: 54%

“…The strong activity of AKPase and G-6-Pase was observed in alveoli of all three orbital glands in present study. However, a weak reaction of AKPase was reported in the alveoli and their ducts in lacrimal gland of neonatal buffalo calves, while a moderate to strong reaction for G-6-Pase was also observed in the alveoli of lacrimal gland in neonatal buffalo calves ( Singh, Malhi & Singh, 1999 ). The AKPase catalyzes the processes of synthesis and transport of substances across the cell membrane and required for the growth, proliferation and differentiation of fetal cells whereas the glucose-6-phosphatase enzyme is responsible for glucose and steroid metabolism ( Nelson & Cox, 2004 ).…”

Section: Discussionmentioning

confidence: 99%

“…The AKPase catalyzes the processes of synthesis and transport of substances across the cell membrane and required for the growth, proliferation and differentiation of fetal cells whereas the glucose-6-phosphatase enzyme is responsible for glucose and steroid metabolism ( Nelson & Cox, 2004 ). The strong activities of these enzymes were suggestive of higher metabolic activity in these glands of buffalo fetuses, although the weak reaction of AKPase enzyme in lacrimal gland of buffalo calf indicates that the cellular proliferation and differentiation was inhibited after birth and shows the completion of differentiation during fetal life only ( Singh, Malhi & Singh, 1999 ).…”

Section: Discussionmentioning

confidence: 99%

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Enzyme histochemical characterization of orbital glands in fetuses of Indian buffalo (Bubalus bubalis)

Tomar¹,

Bansal

2023

PeerJ

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Background The orbital glands, viz. lacrimal gland, superficial and deep gland of third eyelid (LG, SGT and HG), are important for normal eye functions. These glands have different functions in various animals. The information about the enzyme histochemical nature of prenatal orbital glands in Indian buffalo seems to be unavailable. Therefore, the study was planned on orbital glands of six full term recently died fetuses from animals with dystocia. Methods The frozen sections of all these glands were subjected to standard localization protocols for Alkaline Phosphatase (AKPase), Glucose 6 phosphatase (G-6-Pase), Lactate dehydrogenase (LDH), Succinate dehydrogenase (SDH), Glucose 6 phosphate dehydrogenase (G-6-PD), Nicotinamide Adenine Dinucleotide Hydrogen Diaphorase (NADHD), Nicotinamide Adenine Dinucleotide Phosphate Hydrogen diaphorase (NADPHD), Dihydroxy phenylalanine oxidase (DOPA-O), Tyrosinase, non-specific esterase (NSE) and Carbonic anhydrase (CAse). Results The results revealed a mixed spectrum of reaction for the above enzymes in LG, SGT and HG which ranged from moderate (for LDH in SGT) to intense (for most of the enzymes in all three glands). However, DOPA-O, Tyrosinase and CAse did not show any reaction. From the present study, it can be postulated that the orbital glands of fetus have a high activity of metabolism as it has many developmental and functional activities which were mediated with the higher activity of the enzymes involved.

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Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Enzyme histochemical characterization of orbital glands in fetuses of Indian buffalo (Bubalus bubalis)

Tomar¹,

Bansal

2023

PeerJ

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“…For TEM examination, small portions from SMGs (1 mm 3 ) were prepared according to Singh & Singh (2016). Finally, the sections were examined and photographed by TEM (JEOL JEM ® 100CX II, Tokyo, Japan) at various magnifications.…”

Section: Methodsmentioning

confidence: 99%

The Prospective Effect of Cinnamon and Chia on Submandibular Salivary Glands After Ciprofloxacin Administration in Albino Rats (Histological, Histochemical, and Ultrastructural Study)

Abd-Elmonsif

El-Zainy

Rabea

et al. 2022

Microscopy and Microanalysis

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Ciprofloxacin (CPFX®) is potent fluoroquinolone but has severe side effects. Cinnamon (CIN) and chia seeds are potent antioxidants. The current work aimed to compare the effect of CIN extract and chia seeds on CPFX®-treated submandibular salivary glands (SMGs). Thirty-two male albino rats were divided into four groups: Group 1: received saline. Group 2: received CPFX®. Group 3: received CIN extract after 4 h of CPFX® administration. Group 4: received ground chia seeds after 4 h of CPFX® administration. After 10 days, histological, histochemical, and ultrastructural examinations were done. Different examinations illustrated normal features of SMG in Groups 1 and 3. Group 2 showed degenerative signs. Group 4 showed normal features in some areas. Statistical results illustrated that Group 2 had highest mean vacuolation area%. Highest mean of PAS optical density (OD) was for Group 2. Concerning mercuric bromophenol blue stain OD; Group 1 showed highest mean OD. CPFX® has the deteriorative effect on SMG structure and ultrastructure. It leads to increased levels of glycosaminoglycans (GAGs) and decreased levels of total proteins. CIN extract showed more ameliorative effect compared to chia seeds.

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“…Various studies have been done on this gland in buffalo (Venkatakrishnan and Mariappa, 1969;Singh and Singh, 2016), rat (Taga and Sesso, 2002;Uchida et al 2013) and pangolin (Munyala et al 2008), however, there is no detailed information about the histogenesis of sublingual salivary gland of buffalo during prenatal as well as neonatal life. Therefore, the present work was undertaken with an aim to highlight the ontogenetic events in histogenesis of sublingual salivary gland of foetal and neonatal buffalo.…”

Section: Introductionmentioning

confidence: 99%

Developmental Changes in Sublingual Salivary Gland of Indian Buffalo during Prenatal and Neonatal Life

Singh

2021

IJAR

Self Cite

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Background: The sublingual salivary gland being one of the major salivary glands contributes to a substantial amount of saliva secreted into the mouth. Saliva contains water, various enzymes, mucopolysaccharides and lubricating glycoproteins. It has an important role in providing lubrication for eating and vocalization, aid digestion and supply saliva for pH buffering. The study of prenatal development is prerequisite to understand the normal developmental biology of an organ. The documentation of normal foetal growth can serve as a guide for understanding the consequence of harmful influences at various stages of gestation. The present study was aimed at elucidating the histogenesis of sublingual gland of buffalo during prenatal as well as neonatal life.Methods: The study was carried out on 36 buffalo foetuses, during different stages of prenatal development, as well as 6 neonates. The fetuses were categorized into three groups based on their curved crown rump length (CVRL). The ontogenetic events in histogenesis of sublingual salivary gland of foetal and neonatal buffaloes were observed.Result: The primordial anlage of monostomatic and polystomatic parts of sublingual gland was observed at 39th day and 53rd day of development respectively. The primary ducts (interlobular) were first observed at 80th day of development. The lobulation of the gland was started at 107th day of development, whereas the capsule formation was initiated by the aggregation of mesenchymal tissue at 121st day of development. The duct system was completed at 121st day of development. At same day, the terminal tubules attained the structure of the acini. The typical compound tubulo-acinar nature was first observed at 122nd day of development. The gland showed predominantly mucous type of cells at 137th day of development. The myoepithelial cells were first appeared at 138th day of development. The monostomatic and polystomatic parts were clearly distinguishable by connective tissue at 170th day of development. In neonates, the gland was of compound tubulo-acinar nature with a well-defined capsule. Localization of acidic and neutral mucopolysaccharides was observed in mucous and goblet cells. Fine lipid droplets were observed in intralobular as well as interlobular connective tissue, however, phospholipids were found in the cell membrane of secretory acinar cells and ducts.

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Ultrastructural changes in the sublingual salivary gland of prenatal buffalo (Bubalus bubalis)

Cited by 5 publications

References 5 publications

Enzyme histochemical characterization of orbital glands in fetuses of Indian buffalo (Bubalus bubalis)

Enzyme histochemical characterization of orbital glands in fetuses of Indian buffalo (Bubalus bubalis)

The Prospective Effect of Cinnamon and Chia on Submandibular Salivary Glands After Ciprofloxacin Administration in Albino Rats (Histological, Histochemical, and Ultrastructural Study)

Developmental Changes in Sublingual Salivary Gland of Indian Buffalo during Prenatal and Neonatal Life

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