Ultrastructural Changes in the Kidney Cortex of Rats Treated with Lead Acetate

Abstract

SUMMARY:The purpose of this study was to investigate the ultrastructural effects of lead on the kidney cortex of rats. Wistar Albino rats (180-200g body weight) were divided into a controlled and lead acetate-exposed group. Rats received lead acetate at 500 ppm in their drinking water for 60 days. Both groups were fed with the same standard food, but lead acetate was added to the drinking water. During the experimental period, blood samples were taken from the abdominal aorta of the anesthetised animals. At the end of exposure, body weight and blood lead levels were measured. The kidney tissue samples were prepared and analyzed by light and transmission electron microscopy. Cortical renal tubules show various degenerative changes with focal tubular necrosis invaded by inflammatory cells. The ultrastructural alterations found in lead acetate-treated rats were a diminution in the amount of filtration slits, increased fusion of foot processes in epithelial cells of the glomeruli, increase of lysosomal structures and pinocytic vesicles as well as large mitochondria in proximal tubule cells.

Role of garlic extract and silymarin compared to dimercaptosuccinic acid (DMSA) in treatment of lead induced nephropathy in adult male albino rats

“…There was inflammatory infiltration in the renal medulla. These data are in accordance with Deveci et al who reported that chronic lead toxicity induced varying degree of damaged glomeruli and degenerated proximal tubules confirming that lead causes tubulo-interstitial nephritis [29] . The congestion, inflammatory infiltration and atrophied glomeruli in lead acetate treated rats were also reported by Sujatha et al who explained these histopathological changes by the binding of lead to sulfhydryl groups of essential enzymes of cellular metabolism [30] .…”

Tartrazine induces structural and functional aberrations and genotoxic effectsin vivo

“…These results are similar to those of previous studies conducted with food additives, sodium benzoate and citric acid ( Bakar & Aktac, 2014 ; Chen et al, 2014 ; Aktac et al, 2008 ; Kaboglu & Aktac, 2002 ). The presence of pyknotic nuclei and necrosis of hepatocytes and tubular cells in the current work clearly indicates toxicosis as previously described by Deveci et al (2011) and Sarkar & Ghosh (2012) . Structural changes in nuclei and ER, the critical structures for biosynthesis of glycoconjugates, could probably impairs glycosylation mechanisms and hence affect cell surface sialylation ( Bakar & Aktac, 2014 ).…”

Peer Review #1 of "Tartrazine induces structural and functional aberrations and genotoxic effects in vivo (v0.1)"

“…These results are similar to those of previous studies conducted with food additives, sodium benzoate and citric acid (Bakar & Aktac, 2014;Chen et al, 2014;Aktac et al, 2008;Kaboglu & Aktac, 2002). The presence of pyknotic nuclei and necrosis of hepatocytes and tubular cells in the current work clearly indicates toxicosis as previously described by Deveci et al (2011) and Sarkar and Ghosh (2012). Structural changes in nuclei and ER, the critical structures for biosynthesis of glycoconjugates, could probably impairs glycosylation mechanisms and hence affect cell surface sialylation (Bakar & Aktac, 2014).…”