Ultrastructural changes in oocytes during folliculogenesis in domestic mammals

Paulini, Fernanda; Silva, Renata Carvalho; Rôlo, José Luiz Jivago de Paula; Lucci, Carolina Madeira

doi:10.1186/s13048-014-0102-6

Cited by 41 publications

(51 citation statements)

References 104 publications

(200 reference statements)

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“…9,15,16 Alterations in morphology result from complex mechanisms that signal the start or degree of advancement of the atresia process at cellular level, and a wide variety of such alterations have been evaluated to demonstrate their correlation with the atresia signs at histological or ultrastructural level. 23,24 Among histological variables, some that stand out are follicle crenation, the presence of vacuoles, organization of follicular cells around the immature oocyte, and nuclear integrity, all of which were found more frequently in this study. However, the number of morphological alterations increased in follicles treated with OH + NSS.…”

Section: Ultrastructure Of Bovine Preantral Folliclesmentioning

confidence: 60%

“…Even though the atresia signs can accumulate during folliculogenesis, the existence of incomplete development of the metabolic functions, mainly in the immature oocyte mitochondria, has been reported. 24 This prevents the oocyte from adequately dealing with the damage caused by the oxygen reactive species induced by ethanol at the ultrastructural level during asepsis. Additionally, damage becomes more evident with the acceleration of the atresia process.…”

Section: Ultrastructure Of Bovine Preantral Folliclesmentioning

confidence: 99%

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Ultrastructure of bovine preantral follicles: effect of the slaughterhouse aseptic process on follicular integrity

Prado¹,

Palacios

Sedano

et al. 2017

Vet Méx OA

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Ovaries from slaughtered cows are the most abundant source of preantral immature follicles for in vitro studies including maturation and in vitro fertilization. Integrity of immature follicles may be compromised by inadequate management during the collection and transport of the gonads, limiting the success of a correct evaluation. This study assessed the effect of aseptic and transport of bovine ovaries on the structural and ultrastructural integrity of immature follicles from the ovarian cortex. Ovaries from 20 different crossbred cows (Bos taurus × B. indicus) were randomly assigned to one of two aseptic and transport treatments: (1) the ovaries were washed once with 70 % ethanol for 10 s, rinsed twice with normal saline solution (OH + NSS), and then transported in NSS + 0.05 % gentamicin sulphate; or (2) the ovaries were washed three times with phosphate buffered saline (PBS) and transported in PBS + 0.05 % gentamicin sulphate. Samples of the ovarian cortex were obtained from each treatment group. Half of samples of each treatment was processed for histologic evaluation of follicular morphology and developmental stage using haematoxylin & eosin, and the other half was processed to analyse the ultrastructure of immature follicles using transmission electronic microscopy. The rates of good-quality follicles were 36.95 % and 88.6 % for OH + NSS and PBS, respectively. Histological analysis showed higher number of abnormal follicles in OH + NSS than in PBS treated ovaries (F 1,12 = 99.64, P = 0.0001). The proportions of morphological alterations were not significantly different between treatments (χ 2 1,7 = 7.72, P = 0.358). The ultrastructure of immature follicles from OH + NSS ovaries showed cell damage, revealed by abundant cell detritus around both, the oocyte and follicular cells. However, the presence of alterations was barely evident in both the cytoplasm and the nucleus of the granulosa cells.

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Section: Ultrastructure Of Bovine Preantral Folliclesmentioning

confidence: 60%

Section: Ultrastructure Of Bovine Preantral Folliclesmentioning

confidence: 99%

Ultrastructure of bovine preantral follicles: effect of the slaughterhouse aseptic process on follicular integrity

Prado¹,

Palacios

Sedano

et al. 2017

Vet Méx OA

View full text Add to dashboard Cite

show abstract

“…According to Mondadori et al (2010), the location of this organelle into the oocyte varies according to the follicle development and it appears to be more developed in growing follicles than in the early PFs, consistent with the higher metabolism and requirement for protein and lipids of those oocytes (Paulini et al 2014).…”

Section: Discussionmentioning

confidence: 99%

“…The smallest follicles (primordial and primary) were arranged as groups, forming clusters within the connective tissue, similarly as described for rats (Hirshfield 1989) and chinchillas (Sánchez-Toranzo et al 2014). It is well known that the ovarian cortex contains thousands to millions of primordial follicles that constitute the ovarian reserve from which follicles are engaged for development (Paulini et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Estimation, morphometry and ultrastructure of ovarian preantral follicle population in agouti (Dasyprocta leporina)

et al. 2018

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The aim of this study was to characterize the preantral ovarian follicular population in agoutis (D. leporina) by estimating the number of follicles at each developmental category, and also describe the morphometry and the specific features of the follicle and the oocyte by using light and transmission electron microscopy. The length of each ovary was measured using a caliper rule, longitudinally sectioned into two halves and both were immediately fixed to perform the estimation of follicular population and ultrastructural analysis. The mean (±S.E.M.) population of follicular per pair of ovary was estimated at 4419.8±532.26 and 5397.52±574.91 for right and left ovaries, respectively, but no differences were observed between them. The diameters for follicles, oocyte and nuclei were: 18.62±3.40μm, 12.28±2.37μm and 6.10±0.93μm for primordial, 23.75±5.70μm, 14.22±3.00μm and 6.70±1.24μm for primary and 88.55±17.61μm, 52.85±17.56μm and 22.33±17.61μm for secondary follicles, respectively. The most of the follicles found belonged to the primordial category (86.63%), followed by primary (13.01%) and secondary (0.35%) one. Additionally, polyovular follicles were observed in all the animals and they represented 7.51% of the total follicles counted. The ultrastructural analysis showed that the oocyte presented a central and regular nuclei, displaying a homogenous mass. Among the organelles, the mitochondria were the most abundant and the oocyte Golgi apparatus was rarely observed. In conclusion, this work shows for the first time the characterization of the population of preantral follicles in the ovary of Dasyprocta leporina. Those information will be useful for further development and adaptation of biotechniques such as germplasm cryopreservation and in vitro gametes manipulation.

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“…As the ovarian follicle grows, the number of lipid droplets associated with endoplasmic reticulum and mitochondria increase, denoting a rise in oocyte metabolism (rev. [34]). During the transformation from primary to secondary follicle, lipid droplets change from small round dark droplets to large gray structures in pigs, which reflects a change in fatty acids composition [35].…”

Section: Fatty Acid Metabolism Within the Follicular Environmentmentioning

confidence: 99%

Energy metabolism of follicular environment during oocyte growth and maturation

Warzych

Lipińska

2020

Journal of Reproduction and Development

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Oocyte quality is affected by many factors, among which the environment of growth and maturation seems to be crucial. Studies show that well balanced oocyte energy metabolism has a significant impact on several elements of cytoplasmic and nuclear maturation as well as further embryo developmental competence. Therefore homeostasis between metabolism of glucose and fatty acids in the oocyte is being widely described nowadays. This review aims to discuss the follicular (in vivo) or maturation media (in vitro) environments with regard to glucose and fatty acid metabolism, as the main sources of the energy for the oocyte. A great emphasis is given on the balance between those two metabolic pathways and its further impact on female fertility.

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Ultrastructural changes in oocytes during folliculogenesis in domestic mammals

Cited by 41 publications

References 104 publications

Ultrastructure of bovine preantral follicles: effect of the slaughterhouse aseptic process on follicular integrity

Ultrastructure of bovine preantral follicles: effect of the slaughterhouse aseptic process on follicular integrity

Estimation, morphometry and ultrastructure of ovarian preantral follicle population in agouti (Dasyprocta leporina)

Energy metabolism of follicular environment during oocyte growth and maturation

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