Energy metabolism of follicular environment during oocyte growth and maturation

Warzych, Ewelina; Lipińska, Paulina

doi:10.1262/jrd.2019-102

Cited by 67 publications

(50 citation statements)

References 56 publications

(100 reference statements)

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“…During oocyte maturation and development of mammalian embryo, substantially, oxidation of fatty acids provide higher amount of energy in the form of ATP by converting it into acetyl-CoA [ 30 , 31 ]. The in vitro cultured BLs supplemented either with BOECs-CM and Exo were analyzed for lipid droplet accumulation and FAO metabolizing genes’ expression; both groups showed similar expression pattern, but the effect was significantly more pronounced in the BOECs-Exo supplemented group ( Figure 5 ).…”

Section: Discussionmentioning

confidence: 99%

Bovine Oviduct Epithelial Cell-Derived Culture Media and Exosomes Improve Mitochondrial Health by Restoring Metabolic Flux during Pre-Implantation Development

Sidrat

Khan

Joo

et al. 2020

IJMS

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Oviduct flushing is enriched by a wide variety of nutrients that guide the 3–4 days journey of pre-implantation embryo through the oviduct as it develops into a competent blastocyst (BL). However, little is known about the specific requirement and role of these nutrients that orchestrate the early stages of embryonic development. In this study, we aimed to characterize the effect of in vitro-derived bovine oviduct epithelial cell (BOECs) secretion that mimics the in vivo oviduct micro-fluid like environment, which allows successful embryonic development. In this study, the addition of an in vitro derived BOECs-condition media (CM) and its isolated exosomes (Exo) significantly enhances the quality and development of BL, while the hatching ability of BLs was found to be high (48.8%) in the BOECs-Exo supplemented group. Surprisingly, BOECs-Exo have a dynamic effect on modulating the embryonic metabolism by restoring the pyruvate flux into TCA-cycle. Our analysis reveals that Exo treatment significantly upregulates the pyruvate dehydrogenase (PDH) and glutamate dehydrogenase (GLUD1) expression, required for metabolic fine-tuning of the TCA-cycle in the developing embryos. Exo treatment increases the influx into TCA-cycle by strongly suppressing the PDH and GLUD1 upstream inhibitors, i.e., PDK4 and SIRT4. Improvement of TCA-cycle function was further accompanied by higher metabolic activity of mitochondria in BOECs-CM and Exo in vitro embryos. Our study uncovered, for the first time, the possible mechanism of BOECs-derived secretion in re-establishing the TCA-cycle flux by the utilization of available nutrients and highlighted the importance of pyruvate in supporting bovine in vitro embryonic development.

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Section: Discussionmentioning

confidence: 99%

Bovine Oviduct Epithelial Cell-Derived Culture Media and Exosomes Improve Mitochondrial Health by Restoring Metabolic Flux during Pre-Implantation Development

Sidrat

Khan

Joo

et al. 2020

IJMS

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“…The oocyte requires large amounts of energy during its development in preparation for fertilization. There are also energy reserves stored within the mature oocyte to facilitate the initial period of embryogenesis [ 15 ]. Creatine metabolism occurs within human and mouse oocytes.…”

Section: Creatine Metabolism In the Female Reproductive Systemmentioning

confidence: 99%

Creatine Metabolism in Female Reproduction, Pregnancy and Newborn Health

et al. 2021

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Creatine metabolism is an important component of cellular energy homeostasis. Via the creatine kinase circuit, creatine derived from our diet or synthesized endogenously provides spatial and temporal maintenance of intracellular adenosine triphosphate (ATP) production; this is particularly important for cells with high or fluctuating energy demands. The use of this circuit by tissues within the female reproductive system, as well as the placenta and the developing fetus during pregnancy is apparent throughout the literature, with some studies linking perturbations in creatine metabolism to reduced fertility and poor pregnancy outcomes. Maternal dietary creatine supplementation during pregnancy as a safeguard against hypoxia-induced perinatal injury, particularly that of the brain, has also been widely studied in pre-clinical in vitro and small animal models. However, there is still no consensus on whether creatine is essential for successful reproduction. This review consolidates the available literature on creatine metabolism in female reproduction, pregnancy and the early neonatal period. Creatine metabolism is discussed in relation to cellular bioenergetics and de novo synthesis, as well as the potential to use dietary creatine in a reproductive setting. We highlight the apparent knowledge gaps and the research “road forward” to understand, and then utilize, creatine to improve reproductive health and perinatal outcomes.

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“…CCs absorb and convert glucose, transferring further metabolites to the oocyte. With regard to lipid metabolism, CCs are also crucial, accumulating lipids from the environment as a reservoir, on the one hand, and protecting from the lipotoxic effect of selected fatty acids, on the other hand [17]. Therefore, changes in the metabolism of CCs may affect the oocyte and consequently influence embryonic developmental competence.…”

Section: Oocytes Cumulus Cells Oocytes Cumulus Cellsmentioning

confidence: 99%

“…It has been previously reviewed that both glucose and FAs may be utilized by mammalian oocytes; however, homeostasis between the energy metabolism of lipids and carbohydrates was suggested [17]. It is still unknown what is the extent of the oocyte's plasticity, and how far the oocyte may redirect its energy metabolism as a response to the external signals (composition of in vitro maturation media or in vivo changes within the follicular environment).…”

Section: Introductionmentioning

confidence: 99%

Response of Bovine Cumulus–Oocytes Complexes to Energy Pathway Inhibition during In Vitro Maturation

Lipińska

Sell‐Kubiak

Pawlak

et al. 2021

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Glucose or fatty acids (FAs) metabolisms may alter the ovarian follicle environment and thus determine oocyte and the nascent embryo quality. The aim of the experiment was to investigate the effect of selective inhibition of glucose (iodoacetate + DHEA) or FA (etomoxir) metabolism on in vitro maturation (IVM) of bovine COCs (cumulus–oocyte complexes) to investigate oocyte’s development, quality, and energy metabolism. After in vitro fertilization, embryos were cultured to the blastocyst stage. Lipid droplets, metabolome, and lipidome were analyzed in oocytes and cumulus cells. mRNA expression of the selected genes was measured in the cumulus cells. ATP and glutathione relative levels were measured in oocytes. Changes in FA content in the maturation medium were evaluated by mass spectrometry. Our results indicate that only glucose metabolism is substantial to the oocyte during IVM since only glucose inhibition decreased embryo culture efficiency. The most noteworthy differences in the reaction to the applied inhibition systems were observed in cumulus cells. The upregulation of ketone body metabolism in the cumulus cells of the glucose inhibition group suggest possibly failed attempts of cells to switch into lipid consumption. On the contrary, etomoxir treatment of the oocytes did not affect embryo development, probably due to undisturbed metabolism in cumulus cells. Therefore, we suggest that the energy pathways analyzed in this experiment are not interchangeable alternatives in bovine COCs.

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Energy metabolism of follicular environment during oocyte growth and maturation

Cited by 67 publications

References 56 publications

Bovine Oviduct Epithelial Cell-Derived Culture Media and Exosomes Improve Mitochondrial Health by Restoring Metabolic Flux during Pre-Implantation Development

Bovine Oviduct Epithelial Cell-Derived Culture Media and Exosomes Improve Mitochondrial Health by Restoring Metabolic Flux during Pre-Implantation Development

Creatine Metabolism in Female Reproduction, Pregnancy and Newborn Health

Response of Bovine Cumulus–Oocytes Complexes to Energy Pathway Inhibition during In Vitro Maturation

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