Ultrastructural Aspects of Erythropoietic Differentiation in Long-term Bone Marrow Culture

Allen, Terry D.; Dexter, T. M.

doi:10.1111/j.1432-0436.1982.tb01201.x

Cited by 79 publications

(48 citation statements)

References 17 publications

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“…This in vitro culture demonstrates the formation ex vivo of a hemopoietic niche. It should be noted, however, that the cellular structure that forms in vitro is highly complex and extreme diversity in cell phenotypes, and interactions have been reported to occur in long-term bone marrow cultures [42,43]. Apart from the niche-forming ability of bone marrow stroma, at least a fraction of cells within such stroma possess multipotency, that is, can give rise to a variety of mesodermal cell types [11].…”

Section: Discussionmentioning

confidence: 99%

The Myelopoietic Supportive Capacity of Mesenchymal Stromal Cells Is Uncoupled from Multipotency and Is Influenced by Lineage Determination and Interference with Glycosylation

et al. 2008

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Cultured bone marrow stromal cells create an in vitro milieu supportive of long-term hemopoiesis and serve as a source for multipotent mesenchymal progenitor cells defined by their ability to differentiate into a variety of mesodermal derivatives. This study aims to examine whether the capacity to support myelopoiesis is coupled with the multipotency. Our results show that the myelopoietic supportive ability of stromal cells, whether from the bone marrow or from embryo origin, is not linked with multipotency; cell populations that possess multipotent capacity may or may not support myelopoiesis, whereas others, lacking multipotency, may possess full myelopoietic supportive ability. However, upon differentiation, the ability of multipotent mesenchymal progenitors to support myelopoiesis is varied. Osteogenic differentiation did not affect myelopoietic supportive capacity, whereas adipogenesis resulted in reduced ability to support the maintenance of myeloid progenitor cells. These differences were accompanied by a divergence in glycosylation patterns, as measured by binding to lectin microarrays; osteogenic differentiation was associated with an increased level of antennarity of N-linked glycans, whereas adipogenic differentiation caused a decrease in antennarity. Inhibition of glycosylation prior to seeding the stroma with bone marrow cells resulted in reduced capacity of the stromal cells to support the formation of cobblestone areas. Our data show that myelopoietic support is unrelated to the multipotent phenotype of cultured mesenchymal progenitors but is dependent on the choice of differentiation pathway and upon correct glycosylation of the stromal cells.

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Section: Discussionmentioning

confidence: 99%

The Myelopoietic Supportive Capacity of Mesenchymal Stromal Cells Is Uncoupled from Multipotency and Is Influenced by Lineage Determination and Interference with Glycosylation

et al. 2008

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“…This association can be demonstrated both in vivo, following staining of bone marrow with mAbs such as F4/80, and in long-term in vitro bone marrow cultures (20,21). Although these associations are well documented, there is little direct evidence defining the role of macrophages in the developmental regulation of the clustered cells.…”

Section: Msmentioning

confidence: 97%

Interleukin 4 regulates induction of sialoadhesin, the macrophage sialic acid-specific receptor.

McWilliam

Tree

Gordon

1992

Proc. Natl. Acad. Sci. U.S.A.

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S m is a lectinke receptor found on a ried polati of ssue i in lymphoid and hemopoietic times. In bone marrow, it is localized to areas of contact between the resident al macrophages and developing , which together form my- Resident macrophages are distributed throughout the organs and tissues of the body, where they are thought to play a central role in both innate and specific immune responses and in the maintenance of normal homeostatic mechanisms such as hemopoiesis. The precise function of macrophages within specific microenvironments such as the bone marrow or the brain is poorly understood, but it appears that these cells maintain a discrete set of surface receptors which are specific for their particular anatomical/functional location and which appear to be controlled in a spatially and temporally precise manner.The way in which tissue macrophages interact with other cells and with components of the extracellular matrix is of particular interest. Our laboratory has described a macrophage-restricted receptor that has a lectin-like specificity for ligands containing terminal sialic acid residues (1). This receptor, known as SER or sialoadhesin, was characterized by means of its functional capacity to bind sheep erythrocytes in the absence of divalent cations. Following the development of a specific monoclonal antibody (mAb), , the receptor was isolated by affinity chromatography and shown to be a glycoprotein of 185 kDa (reduced) or 175 kDa (nonreduced) (3). The isolated protein at nanomolar concentrations was capable of agglutinating sheep and human erythrocytes, and this agglutination could be inhibited with gangliosides such as GT1b and GD1a, suggesting that the receptor reacts preferentially with oligosaccharides containing terminal sialic acid as

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“…In the erythroblastic islands, a central macrophage provides favorable proliferative and survival signals to the surrounding erythroblasts, and it eventually engulfs the extruded nuclei of maturing erythrocytes. [2][3][4][5] Inhibition of the interaction between macrophage and erythroblasts usually leads to embryonic anemia accompanied by accelerated apoptosis of erythroid cells. Targeted disruption of the gene palld, which encodes actin cytoskeleton associated protein (palladin) prevented effective erythroblast-macrophage interactions because of differentiation defects in the macrophage.…”

Section: Introductionmentioning

confidence: 99%

c-Maf plays a crucial role for the definitive erythropoiesis that accompanies erythroblastic island formation in the fetal liver

Kusakabe

Hasegawa²,

Hamada³

et al. 2011

Blood

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Ultrastructural Aspects of Erythropoietic Differentiation in Long-term Bone Marrow Culture

Cited by 79 publications

References 17 publications

The Myelopoietic Supportive Capacity of Mesenchymal Stromal Cells Is Uncoupled from Multipotency and Is Influenced by Lineage Determination and Interference with Glycosylation

The Myelopoietic Supportive Capacity of Mesenchymal Stromal Cells Is Uncoupled from Multipotency and Is Influenced by Lineage Determination and Interference with Glycosylation

Interleukin 4 regulates induction of sialoadhesin, the macrophage sialic acid-specific receptor.

c-Maf plays a crucial role for the definitive erythropoiesis that accompanies erythroblastic island formation in the fetal liver

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