“…Although an abnormal level of circulating nucleated definitive cells are observed in embryos deficient for the DNASE2A, MAF or palladin macrophage proteins (Kawane et al, 2001;Kusakabe et al, 2011;Liu et al, 2007), ablating the majority of macrophage cells does not automatically yield an abundance of nucleated red cells in circulation (Chow et al, 2013;Ramos et al, 2013). In addition, there are a number of structural, enzymatic and chromatin-associated erythroid cell factors that play an intrinsic role in morphologically preparing the cell for proper nuclear extrusion (Ji et al, 2011;Keerthivasan et al, 2011;Konstantinidis et al, 2012;Ney, 2011;Ubukawa et al, 2012;von Lindern, 2006).…”