Ultrastructural Aspects and Possible Origin of Cytoplasmic Channels Providing Intercellular Connection in Vegetative Tissues of Anthers<sup>1</sup>

Wang, Xin Yu; Yu, Chun Hong; Li, Xin; Wang, Chong Ying; Zheng, Guo Chang

doi:10.1023/b:rupp.0000011308.61339.fe

Cited by 30 publications

(20 citation statements)

References 29 publications

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“…Recently, it has also been reported in transgenic plants of Nicotiana tabacum (Sidorchuk et al 2007). Cytomixis is more commonly observed in meiotic cells but it has also been reported to occur in the somatic cells (Kostritsyna and Soldatov 1991, Wang et al 2004, Guzicka and Wozny 2005.…”

Section: ⎯⎯⎯⎯mentioning

confidence: 96%

Cytomixis and associated meiotic abnormalities affecting pollen fertility in Clematis orientalis

Kumar¹,

Singhal²,

Kaur³

et al. 2010

Biologia plant.

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Present cytological investigations from the cold desert regions of Lahaul-Spiti and Kinnaur (India) record the first ever tetraploid (2n=32) chromosome count and cytomixis in Clematis orientalis L. var. acutifolia Hook. f. et Thoms. The phenomenon of cytomixis (9.33 -29.80 %) involving chromatin transfer among 2 -3 proximate pollen mother cells (PMCs) during male meiosis occurs through narrow and broad cytoplasmic channels from early prophase to tetrad stage. However, frequency of its occurrence during the later meiotic stages is rather low. Chromatin transfer results into the formation of hypo-, hyperploid and enucleated PMCs. Various meiotic abnormalities associated with cytomixis such as chromatin stickiness, pycnotic chromatin, interbivalent connections, out of plate bivalents, late disjunction of bivalents, and laggards and bridges resulted into some pollen sterility (16.33 -49.30 %) and heterogeneous pollen grains size.

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Section: ⎯⎯⎯⎯mentioning

confidence: 96%

Cytomixis and associated meiotic abnormalities affecting pollen fertility in Clematis orientalis

Kumar¹,

Singhal²,

Kaur³

et al. 2010

Biologia plant.

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“…After it was first recorded by Arnoldi (1900), the phenomenon of cytomixis and other abnormalities responsible for abnormal meiotic behavior and reduced pollen fertility have been reported in a number of flowering plants (Vorsa and Bingham 1979, Singhal and Gill 1985, Mendes-Bonato et al 2002, Malallah and Attia 2003, Lattoo et al 2006, Singhal and Kumar 2008a, b, 2010, 2010, b, 2014, Singhal et al 2009, b, Himshikha et al 2010, Kumar 2010, Kravets 2013, Rana et al 2013. At an ultrastructural level, researchers have observed migration of nuclei through single or multiple chromatin channels, chromatin condensation in chromatin channels, restoration of its structure in the recipient cell, formation of micronuclei, and formation of cell chains connected via migrating nuclei (Feijo and Pais 1989, Wang et al 2004, Mursalimov and Deineko 2011, Mursalimov et al 2013a. The extra chromatin masses resulting from the transferred chromatin, usually located away from the main chromosome complement, showed delayed division and contributed to the formation of chromosomal laggards, micronuclei and pollen grains of different sizes, and most importantly pollen sterility.…”

Section: Discussionmentioning

confidence: 99%

Cytomixis and Associated Abnormalities during Male Meiosis in Lindelofia longiflora var. falconeri (Boraginaceae)

2014

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Summary Detailed male meiotic studies were made in Lindelofia longiflora (Royle ex Benth.) Baill. var. falconeri (Cl.) Brand (Family: Boraginaceae) from the cold deserts of Pangi Valley, Chamba District (Himachal Pradesh) in India. The meiotic course in some of the individuals was observed to be highly abnormal which include cytomixis involving chromatin transfer among meiocytes, spindle irregularities, chromatin stickiness and mutlipolar PMCs, resulting in abnormal sporads as dyads, triads, polyads and tetrads with micronuclei. The products of such sporads result in high pollen sterility (77%) and variable-sized pollen grains. As a consequence of cytomixis, hyperploid (73.04%), hypoploid (18.59%) and enucleated PMCs (8.37%) are resulted. The enucleated PMCs may degenerate during the later stages of meiosis while hypo-and hyperploid PMCs yielded pollen grains of variable sizes and sterile pollen grains. The phenomenon of cytomixis and associated meiotic irregularities seem to have been caused due to low temperature stress conditions prevailing in the area.

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“…Já em outros casos, a conexão citoplasmática parecia ocorrer entre várias células ao mesmo tempo, sendo que, umas funcionavam como doadoras e outras como receptoras. Em tecidos da antera de tabaco, Wang et al (2004) observaram que uma célula mãe de grão de pólen pode aceitar material genético de uma célula via canal citomítico enquanto direciona seu material para outra célula vizinha, tal fenômeno também foi observado no presente trabalho. curcas.…”

Section: Comportamento Meióticounclassified

Comportamento meiótico e viabilidade polínica na espécie Jatropha curcas L.

et al. 2015

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ResumoA espécie Jatropha curcas, conhecida como pinhão-manso, pertencente à família Euphorbiaceae, figura atualmente como uma das oleaginosas de maior potencial para a produção de biodiesel no Brasil. O presente estudo teve como objetivo avaliar a meiose e a viabilidade polínica em acessos de J. curcas que compõem a coleção de germoplasma da UFRRJ. Para tal, foram coletados botões florais de 20 plantas, a seguir estes foram fixados em solução de ácido acético 3:1 e álcool 70%. A coloração das células foi feita através dos corantes carmim acético 2% e da solução tripla de Alexander, para as análises meióticas e de viabilidade polínica, respectivamente. Todas as observações foram realizadas em microscópio óptico. Foi observado que a espécie possui 2n = 2x = 22 cromossomos. Foram observadas várias irregularidades durante a divisão meiótica, como a presença de cromossomos pegajosos, produtos meióticos irregulares, como mônades, díades, tríades e políades, além da ocorrência de citomixia e micronúcleos, sendo que estas duas últimas anomalias tiveram maior frequência entre os acessos avaliados. O índice meiótico foi estimado em 84,21% e a média da viabilidade polínica em 89,85%. A menor viabilidade polínica foi de 78% (UFRRJ885), enquanto que a máxima observada foi de 96% (UFRRJ861). Com base nos resultados obtidos, apesar de Jatropha curcas apresentar certas anomalias durante a meiose, os acessos aqui analisados mostraram viabilidade polínica satisfatória. Palavras-chave: Citomixia, índice meiótico, irregularidade meiótica, meiose AbstractThe Jatropha curcas, known as physic nut, belonging to the Euphorbiaceae family, figure today as one of the greatest potential of oilseeds for biodiesel production in Brazil. The present study aimed to evaluate meiosis and pollen viability in J. curcas accesses from UFRRJ germplasm collection. To do that, flower buds were collected from 20 plants, these were fixed in acetic acid 3:1 solution and alcohol 70%. The staining of the cells was done by acetic carmine 2% solution and Alexander's triple solution, for the analysis of meiotic and pollen viability, respectively. All observations were carried out under an optical microscope. It was observed that the species has 2n = 2x = 22 chromosomes. It was observed many irregularities during the meiotic division as the presence of sticky chromosomes and irregular meiotic products, such as monads, dyads, triads and polyads, and the occurrence of cytomixis and micronucleus, these latter two anomalies had a higher frequency among the accessions. The meiotic

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Ultrastructural Aspects and Possible Origin of Cytoplasmic Channels Providing Intercellular Connection in Vegetative Tissues of Anthers¹

Cited by 30 publications

References 29 publications

Cytomixis and associated meiotic abnormalities affecting pollen fertility in Clematis orientalis

Cytomixis and associated meiotic abnormalities affecting pollen fertility in Clematis orientalis

Cytomixis and Associated Abnormalities during Male Meiosis in Lindelofia longiflora var. falconeri (Boraginaceae)

Comportamento meiótico e viabilidade polínica na espécie Jatropha curcas L.

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Ultrastructural Aspects and Possible Origin of Cytoplasmic Channels Providing Intercellular Connection in Vegetative Tissues of Anthers1

Cited by 30 publications

References 29 publications

Cytomixis and associated meiotic abnormalities affecting pollen fertility in Clematis orientalis

Cytomixis and associated meiotic abnormalities affecting pollen fertility in Clematis orientalis

Cytomixis and Associated Abnormalities during Male Meiosis in Lindelofia longiflora var. falconeri (Boraginaceae)

Comportamento meiótico e viabilidade polínica na espécie Jatropha curcas L.

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Ultrastructural Aspects and Possible Origin of Cytoplasmic Channels Providing Intercellular Connection in Vegetative Tissues of Anthers¹