Ultrastructural and optical characteristics of cancer cells treated by a nanotechnology based chemo-photothermal therapy method

Alamzadeh, Zahra; Beik, Jaber; Mahabadi, Vahid Pirhajati; Ardekani, Ali; Ghader, Alireza; Kamrava, Seyed Kamran; Dezfuli, Amin Shiralizadeh; Ghaznavi, Habib; Shakeri‐Zadeh, Ali

doi:10.1016/j.jphotobiol.2019.01.005

Cited by 65 publications

(35 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5 Some studies have reported enhanced cytotoxic effects of drugs such as cisplatin and bortezomib when used in combination with MFH, inducing sensitivity in resistant cancer cells. [6][7][8] In vivo studies have also demonstrated the thermal potentiation of drugs like cisplatin and doxorubicin via MFH, resulting in significant tumor volume reductions in mice. 9,10 Recently, Court et al investigated the thermal potentiation of 2-phenylethynesulfonamide (PES), an inhibitor of the function of the heat-shock proteins (HSP70).…”

Section: Introductionmentioning

confidence: 99%

In vitro Ultrasonic Potentiation of 2-Phenylethynesulfonamide/Magnetic Fluid Hyperthermia Combination Treatments for Ovarian Cancer

Mérida¹,

Rinaldi²,

Juan³

et al. 2020

IJN

View full text Add to dashboard Cite

Background: Magnetic Fluid Hyperthermia (MFH) is a promising adjuvant for chemotherapy, potentiating the action of anticancer agents. However, drug delivery to cancer cells must be optimized to improve the overall therapeutic effect of drug/MFH combination treatments. Purpose: The aim of this work was to demonstrate the potentiation of 2-phenylethynesulfonamide (PES) at various combination treatments with MFH, using low-intensity ultrasound as an intracellular delivery enhancer. Methods: The effect of ultrasound (US), MFH, and PES was first evaluated individually and then as combination treatments. Definity ® microbubbles and polyethylene glycol (PEG)-coated iron oxide nanoparticles were used to induce cell sonoporation and MFH, respectively. Assessment of cell membrane permeabilization was evaluated via fluorescence microscopy, iron uptake by cells was quantified by UV-Vis spectroscopy, and cell viability was determined using automatic cell counting. Results: Notable reductions in cancer cell viability were observed when ultrasound was incorporated. For example, the treatment US+PES reduced cell viability by 37% compared to the non-toxic effect of the drug. Similarly, the treatment US+MFH using mild hyperthermia (41°C), reduced cell viability by an additional 18% when compared to the effect of MH alone. Significant improvements were observed for the combination of US+PES+MFH with cell viability reduced by an additional 26% compared to the PES+MFH group. The improved cytotoxicity was attributed to enhanced drug/nanoparticle intracellular delivery, with iron uptake values nearly twice those achieved without ultrasound. Various treatment schedules were examined, and all of them showed substantial cell death, indicating that the time elapsed between sonoporation and magnetic field exposure was not significant. Conclusion: Superior cancer cell-killing patterns took place when ultrasound was incorporated thus demonstrating the in vitro ultrasonic potentiation of PES and mild MFH. This work demonstrated that ultrasound is a promising non-invasive enhancer of PES/MFH combination treatments, aiming to establish a sono-thermo-chemotherapy in the treatment of ovarian cancer.

show abstract

Section: Introductionmentioning

confidence: 99%

In vitro Ultrasonic Potentiation of 2-Phenylethynesulfonamide/Magnetic Fluid Hyperthermia Combination Treatments for Ovarian Cancer

Mérida¹,

Rinaldi²,

Juan³

et al. 2020

IJN

View full text Add to dashboard Cite

show abstract

“…Several combination therapies can improve the therapeutic effects of cancer, such as chemo-photothermal therapy, which converts light into heat and improves the efficacy of chemotherapeutic drugs. 33,34 Our strategy is to combine two chemotherapeutic drugs simultaneously for bulky breast cancer cells and BCSCs, which can maximize the therapeutic efficacy of chemotherapy and minimize the MDR without special laser excitation. 10,[35][36][37][38] In this study, we chose DTX and SAL for combination therapy to enhance the therapeutic effect of breast cancer.…”

Section: Discussionmentioning

confidence: 99%

Co-Delivery of Docetaxel and Salinomycin to Target Both Breast Cancer Cells and Stem Cells by PLGA/TPGS Nanoparticles

Gao¹,

Liu²,

Xie³

et al. 2019

IJN

View full text Add to dashboard Cite

Conventional chemotherapy is hampered by the presence of breast cancer stem cells (BCSCs). It is crucial to eradicating both the bulky breast cancer cells and BCSCs, using a combination of conventional chemotherapy and anti-CSCs drugs. However, the synergistic ratio of drug combinations cannot be easily maintained in vivo. In our previous studies, we demonstrated that the simultaneous delivery of two drugs via nanoliposomes could maintain the synergistic drug ratio for 12 h in vivo. However, nanoliposomes have the disadvantage of quick drug release, which makes it difficult to maintain the synergistic drug ratio for a long time. Herein, we developed a co-delivery system for docetaxel (DTX)-a first-line chemotherapy drug for breast cancer-and salinomycin (SAL)-an anti-BCSCs drug-in rigid nanoparticles constituted of polylactide-co-glycolide/D-alpha-tocopherol polyethylene glycol 1000 succinate (PLGA/TPGS). Methods: Nanoparticles loaded with SAL and DTX at the optimized ratio (NSD) were prepared by the nanoprecipitation method. The characterization, cellular uptake, and cytotoxicity of nanoparticles were investigated in vitro, and the pharmacokinetics, tissue distribution, antitumor and anti-CSCs activity of nanoparticles were evaluated in vivo. Results: We demonstrated that a SAL/DTX molar ratio of 1:1 was synergistic in MCF-7 cells and MCF-7-MS. Moreover, the enhanced internalization of nanoparticles was observed in MCF-7 cells and MCF-7-MS. Furthermore, the cytotoxicity of NSD against both MCF-7 cells and MCF-7-MS was stronger than the cytotoxicity of any single treatment in vitro. Significantly, NSD could prolong the circulation time and maintain the synergistic ratio of SAL to DTX in vivo for 24 h, thus exhibiting superior tumor targeting and anti-tumor activity compared to other treatments. Conclusion: Co-encapsulation of SAL and DTX in PLGA/TPGS nanoparticles could maintain the synergistic ratio of drugs in vivo in a better manner; thus, providing a promising strategy for synergistic inhibition of breast cancer.

show abstract

“…Interestingly, PTTcombined Dox presented more efficient inhibition of tumor growth than PTT or Dox alone, and PTT combined CQ and Dox could achieve the most efficient inhibition of tumor growth, while a series of studies by Ali Shakeri-Zadeh et al also supported that the combination of PTT and chemotherapy could achieve synergistic therapeutic outcome and be promising in cancer therapy. [38][39][40][41][42][43][44] Given this finding, we thought it is promising to change the current situation of the chemoresistance of GBC. In combination with PTT and CQ, it is possible to reduce the dosage of chemotherapeutic drugs while achieving equivalent or even better therapeutic effect.…”

Section: The Cytotoxic Effect Of Ptt Could Be Enhanced By Chemotherapmentioning

confidence: 96%

Gallbladder Cancer Progression Is Reversed by Nanomaterial-Induced Photothermal Therapy in Combination with Chemotherapy and Autophagy Inhibition

Cai¹,

Wang²,

Wang³

et al. 2020

IJN

View full text Add to dashboard Cite

Introduction: Gallbladder cancer (GBC) is the most common malignancy in biliary tract with extremely poor prognosis. Photothermal therapy (PTT) shows great promises for tumor therapy, which causes tumor cell death via selectively directed heating released by nanoparticles under the near-infrared irradiation. Through degrading damaged organelles and misfolded proteins in autophagosomes, autophagy plays a vital role in maintaining the intracellular homeostasis. The present study attempted to combine chemotherapy and autophagy blocking with PTT. Materials and Methods: We purchased multi-walled carbon nanotubes from Nanostructured and Amorphous Materials and performed PTT using an 808-nm diode laser. The cytotoxic effects of PTT and chemotherapy in vitro were assessed by cell viability analysis. The effects of PTT and chemotherapy on autophagy in vitro were assessed by GFP-LC3 and Western blot. And these results were confirmed by in vivo experiment. Results: Both PTT and chemotherapy could trigger cytoprotective autophagy to tolerate the cellular stresses and prolong the survival of GBC cell; therefore, the blocking of autophagy could enhance the efficacy of PTT and chemotherapy in GBC treatment in vitro and in vivo. Conclusion: Chemotherapeutic drug doxorubicin and autophagy inhibitor chloroquine could enhance the efficacy of nanoparticle-mediated hyperthermia in GBC.

show abstract

Ultrastructural and optical characteristics of cancer cells treated by a nanotechnology based chemo-photothermal therapy method

Cited by 65 publications

References 15 publications

<p>In vitro Ultrasonic Potentiation of 2-Phenylethynesulfonamide/Magnetic Fluid Hyperthermia Combination Treatments for Ovarian Cancer</p>

<p>In vitro Ultrasonic Potentiation of 2-Phenylethynesulfonamide/Magnetic Fluid Hyperthermia Combination Treatments for Ovarian Cancer</p>

<p>Co-Delivery of Docetaxel and Salinomycin to Target Both Breast Cancer Cells and Stem Cells by PLGA/TPGS Nanoparticles</p>

<p>Gallbladder Cancer Progression Is Reversed by Nanomaterial-Induced Photothermal Therapy in Combination with Chemotherapy and Autophagy Inhibition</p>

Contact Info

Product

Resources

About