Ultrastructural and Metabolic Features of Stress-Adaptive Rearrangements of Myocardium in Infants after Surgery-Induced Up-Regulation of Lipoperoxidation Processes

Abstract

Surgical correction of complicated congenital heart disease in infants is terminated by postischemic reperfusion of myocardium accompanied with closure of more than 20% microvessels in the coronary bed by edematous endothelial cells, their bleb-like fragments, and aggregates of the blood formed elements. Degradation of the transmission capacity of the coronary microvessels developed in parallel with moderation of activity of the antiradical protection enzymes and positively correlated with the level of LPO secondary products, whose elevation during the surgery stages was not reduced by catalase activation.

Circulating Reactive Oxygen Species in Adults with Congenital Heart Disease

“…In our study, only five adult patients with VSD were included; this subgroup was too small to perform a separate statistical analysis. In contrast, in most other studies on oxidative stress in the blood of patients with CHD, indirect nonspecific markers of oxidative stress [ 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 ] were used, e.g., the assessment of the activity of oxidative and antioxidant enzymes (superoxide dismutase (SOD), catalase and glutathione peroxidase), levels of antioxidants (vitamin E, uric acid and selenium), levels of malondialdehyde (MDA) and protein carbonyl (PCO), plasma total oxidant status (TOS), total antioxidant capacity or status (TAC or TAS) and oxidative stress index (OSI) as well as the quantification of DNA damage assessed by alkaline comet assay in circulating lymphocytes and levels of 8-hydroxy-2′-deoxyguanosine.…”