Circulating Reactive Oxygen Species in Adults with Congenital Heart Disease

Abstract

Oxidative stress is an important pathophysiological mechanism in the development of numerous cardiovascular disorders, but few studies have examined the levels of oxidative stress in adults with congenital heart disease (CHD). The objective of this study was to investigate oxidative stress levels in adults with CHD and the association with inflammation, exercise capacity and endothelial function. To this end, 36 adults with different types of CHD and 36 age- and gender-matched healthy controls were enrolled. Blood cell counts, hs-CRP, NT-proBNP, fasting glucose, cholesterol levels, iron saturation and folic acid concentrations were determined in venous blood samples. Levels of superoxide anion radical in whole blood were determined using electron paramagnetic resonance spectroscopy in combination with the spin probe CMH. Physical activity was assessed with the IPAQ-SF questionnaire. Vascular function assessment (EndoPAT) and cardiopulmonary exercise testing were performed in the patient group. Superoxide anion radical levels were not statistically significantly different between adults with CHD and the matched controls. Moreover, oxidative stress did not correlate with inflammation, or with endothelial function or cardiorespiratory fitness in CHD; however, a significant negative correlation with iron saturation was observed. Overall, whole blood superoxide anion radical levels in adults with CHD were not elevated, but iron levels seem to play a more important role in oxidative stress mechanisms in CHD than in healthy controls. More research will be needed to improve our understanding of the underlying pathophysiology of CHD.

Inhibition of cAMP signaling prevents congenital heart defects counteracting oxidative stress in Pde2A deficient embryos

“…It is also well known that inflammatory cytokines and oxidative stress levels are observed in several cardiovascular diseases 37,38 , however few inconclusive works have reported the role of inflammation and oxidative stress in CHDs in neonates and adults [39][40][41][42] .…”

Toxic metals in pregnancy and congenital heart defects. Insights and new perspectives for a technology-driven reduction in food sources

“…Additionally, MMA(III) disrupted Smad2/3 activation at a dose 20 times lower than As(III) does [60]. Also, oxidative stress, defined as the imbalance between the production of reactive oxygen species (ROS) and antioxidant defenses, is a relevant pathophysiological mechanism in the development of many cardiovascular disorders [61,62]. Despite the heterogeneity of biomarkers and subgroups of diseases analyzed and the conflicting results between studies, the disruption of redox signaling pathways could have a causative role in CHD [62,[63][64][65][66].…”

The Effect of a Magnetic Field on the Transport of Functionalized Magnetite Nanoparticles into Yeast Cells

“…If the resonance condition is met and an appropriate quantum of radiation with energy corresponding to the energy difference between these levels is supplied to the system, the ESR phenomenon will be registered as the first derivative of the absorption signal [19][20][21]. It has been successfully applied in various studies, including those investigating the properties of magnetic nanoparticles [22][23][24][25][26][27], iron complexes and free radicals in blood and tissues [28][29][30][31][32][33][34][35], as well as geological and archaeological materials [36][37][38][39]. ESR studies confirm the penetration of nanoparticles into cells and facilitate monitoring of this whole process.…”