Ultrastructural analysis of β-lapachone-induced surface membrane damage in male adult Schistosoma mansoni BH strain worms

Aires, André de Lima; Ximenes, Eulália Camelo Pessoa Azevedo; Silva, Renata Alexandre Ramos; Barbosa, Vanessa Xavier; Góes, Alexandre José da Silva; Peixoto, Christina Alves; Souza, Valdênia Maria Oliveira de; Albuquerque, Mônica Camelo Pessôa de Azevedo

doi:10.1016/j.exppara.2014.04.010

Cited by 21 publications

(11 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Generally, if a drug induces tegumental alterations, the onset and precise pattern of these alterations depend on the particular drug and the treatment regimen used (44) . The characteristic features of tegumental damage observed in this study, including destruction of the tegument and erosion and loss of spines, were consistent with those previously described in the literature following in vitro exposure to different antischistosomal agents such as hypericin (45) , allicin (46) , nerolidol (47) , phthalyl thiazole (LpQM-45) (48) , β-lapachone (49) , and DNK (32) . Hence, reductions in worms may be attributed to the tegumental damage observed.…”

Section: Discussionsupporting

confidence: 76%

In vitro antischistosomal activity of venom from the Egyptian snake Cerastes cerastes

Hassan

Abdel-Rahman

Ibrahim

et al. 2016

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

Introduction:We studied the potential in vitro antischistosomal activity of Cerastes cerastes venom on adult Schistosoma mansoni worms. Methods: Live specimens of the horned viper snake, C. cerastes were collected from the Aswan Governorate (Egypt). Venom was collected from snakes by manual milking. Worms of S. mansoni were obtained from infected hamsters by perfusion and isolated from blood using phosphate buffer. Mortality rates of worms were monitored after 3 days of exposure to snake venom at LC 50 and various sublethal concentrations (10, 5, 2.5µg/ml). Scanning electron microscopy was used to investigate tegumental changes in treated worms after exposure to LC 50 doses of venom. Results: The LC 50 of C. cerastes venom was 21.5µg/ml. The effect of C. cerastes venom on Schistosoma worms varied according to their sex. The mortality rate of male and female worms after 48-h exposure was 83.3% and 50% , respectively. LC 50 of C. cerastes venom induced mild to severe tegumental damage in Schistosoma worms in the form of destruction of the oral sucker, shrinkage and erosion of the tegument, and loss of some tubercle spines. Conclusions: The present study demonstrated that C. cerastes venom exerts potential in vitro antischistosomal activity in a time and dose-dependent manner. These results may warrant further investigations to develop novel schistosomicidal agents from C. cerastes snake venom.

show abstract

Section: Discussionsupporting

confidence: 76%

In vitro antischistosomal activity of venom from the Egyptian snake Cerastes cerastes

Hassan

Abdel-Rahman

Ibrahim

et al. 2016

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

show abstract

“…At the end of the 19th century, the conversion of lapachol into alpha-Lapachone and beta-Lapachone (βLP) by acid cyclization was described (Silva et al 2007). βLP (3.4-dihydro-2, 2-dimethyl-2H-naphthol [1.2-b] pyran-5.6-dione), a substance used in this study, has antibacterial, antifungal and antiretroviral activity (Pardee et al 2002, Aires et al 2014. It is known that this compound has good results in prolonging the survival of leukemia models, blocking the multiplication of human immunodeficiency virus (HIV-1), as adjuvant therapy in proliferative retinopathy, as an anti-psoriatic agent, and has schistosomicidal activity (Pardee et al 2002, Aires et al 2014.…”

Section: Introductionmentioning

confidence: 99%

“…βLP (3.4-dihydro-2, 2-dimethyl-2H-naphthol [1.2-b] pyran-5.6-dione), a substance used in this study, has antibacterial, antifungal and antiretroviral activity (Pardee et al 2002, Aires et al 2014. It is known that this compound has good results in prolonging the survival of leukemia models, blocking the multiplication of human immunodeficiency virus (HIV-1), as adjuvant therapy in proliferative retinopathy, as an anti-psoriatic agent, and has schistosomicidal activity (Pardee et al 2002, Aires et al 2014. The fact that it is inhibiting DNA topoisomerase I, an enzyme that plays an important role in the processes of replication and packaging of DNA, triggered studies on the antineoplastic action of βLP in tumors implants in in vivo mice, such as cancers of prostate, ovary and breast, as well as in human cells of in vitro cultivation, such as prostate and lung (Pardee et al 2002, Kung et al 2014.…”

Section: Introductionmentioning

confidence: 99%

β Lapachone blocks the cell cycle and induces apoptosis in canine osteosarcoma cells

et al. 2018

View full text Add to dashboard Cite

Osteosarcoma is a malignant tumor of primitive bone cells with a high incidence in dogs and humans. The need for more effective drugs with less adverse consequences has pushed the development of chemotherapeutic agents from plants and other natural sources. The aim of this study was to verify the cytotoxic effects of β-lapachone, a compound present in the sawdust of Tabebuia sp. (popularly known as ipê) wood, on canine osteosarcoma cells subcultured and treated in different concentrations (0.1μm, 0.3μm e 1.0μm) and exposure times (24h, 48h e 72h). Results were obtained through Trypan blue dye exclusion, tetrazolium reducing method, cell survival assay, Annexin V-FITC and Propidium Iodine labeling, JC-1 dye labeling and cell cycle kinetics e analysis. The group treated with 0.3μm β-lapachone presented higher decrease in cell viability (80.27%, 24h, 47.41%, 48h and 35.19%, 72h) and greater progression of cytotoxicity (19.73%, 24h, 52.59%, 48h and 64.81%, 72h). The lower IC50 (0.180μm) was verified in the group treated for 72 hours. Cell growth after treatment decreased as concentration and time of exposure increased, with 0.50% survival fraction at the concentration of 1.0μm. Initial apoptosis was the most frequent type of cell death in all groups, reaching bottom in the 24-hour group treated with 0.1μm (4.26%) and peaking in the 72-hour group treated with 1.0μm (85.89%). Mitochondrial depolarization demonstrated a dose-dependent phenomenon, indicating the intrinsic apoptosis. Cell growth inhibition by blocking cell cycle in the G0/G1 phase related to the exposure the time. β-lapachone is cytotoxic for canine osteosarcoma cells, induces apoptosis and promotes cell cycle arrest in G0/G1 phase.

show abstract

“…The oil obtained (0.1%) was dried over anhydrous sodium sulfate in the usual manner and stored at 4°C 13 . We used a gas chromatograph coupled to a mass spectrometer (Shimadzu QP-5000) under the following analytical conditions: capillary column, OV-5 (30m× 0.25mm × 0.25μm); injector (Ohio Valley Specialty Chemical, Inc.), 240°C; detector, 230°C; electron impact, 70 eV; gasdrag, He; flow, 1.0mL/min -1 ; split, 1/20; program temperature, 60-240°C at 3°C min -1 ; and solution injection volume, 1μL (1μL of essential oil per 1mL of ethyl acetate).…”

Section: Isolation and Identification Of Rotundifolonementioning

confidence: 99%

“…Investigative methods such as scanning electron microscopy (SEM) have been employed to study the effects of compounds on the tegument of many helminthes 13 , especially S. mansoni 14 . In this context, the search for natural bioactive compounds against S. mansoni offers a potentially valuable approach 15,16 .…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural study of morphological changes in Schistosoma mansoni after in vitro exposure to the monoterpene rotundifolone

Rocha

Cavalcanti

Barbosa-Filho

et al. 2017

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

Introduction: Schistosomiasis, a parasitic disease caused by trematode flatworms of the genus Schistosoma, affects more than 200 million people worldwide, and its control is dependent on a single drug, praziquantel. Here, we report the in vitro effect of rotundifolone, a monoterpene isolated from Mentha x villosa (Lamiaceae), on Schistosoma mansoni adult worms. Methods: The in vitro effect of rotundifolone on adult Schistosoma mansoni was evaluated by analysis of behavior and mortality and through a scanning electron microscopic analysis of ultrastructural changes in the tegument of the worms. Results: At concentrations of 3.54 and 7.09μg/mL -1 rotundifolone, no worm mortality was observed at any of the sampling intervals. A minor reduction in movement of the tail, suckers, and gynecophoral canal membrane was observed after 96 h of exposure to 7.09μg/mL -1 rotundifolone. At 70.96μg/mL -1 , a lack of movement was observed from 72h onwards and all worms were deemed dead; similar effects were observed at 48h with 177.4μg/mL -1 , and at 24h with 354.8μg/mL -1 and 700.96μg/mL -1 . Rotundifolone also caused death of all parasites and separation of coupled pairs into individual males and females after 24h at 354.8μg/mL -1 . Conclusions: The main changes in the tegument induced by the different ROT treatments were: after 24h incubation, bubble lesions spread over the entire body and loss of tubercles occurred in some regions of the ventral region.

show abstract

Ultrastructural analysis of β-lapachone-induced surface membrane damage in male adult Schistosoma mansoni BH strain worms

Cited by 21 publications

References 54 publications

In vitro antischistosomal activity of venom from the Egyptian snake Cerastes cerastes

In vitro antischistosomal activity of venom from the Egyptian snake Cerastes cerastes

β Lapachone blocks the cell cycle and induces apoptosis in canine osteosarcoma cells

Ultrastructural study of morphological changes in Schistosoma mansoni after in vitro exposure to the monoterpene rotundifolone

Contact Info

Product

Resources

About