Ultrastructural analysis of the effect of mating delay on cell death in the ovaries of virgin honey bee (<i>Apis mellifera</i> L.) queens.

Berger, Bruno; Cruz-Landim, Carminda da

doi:10.3896/ibra.1.48.1.12

Cited by 9 publications

(6 citation statements)

References 8 publications

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“…113 If the virgin queen does not mate after 15 days of emergence some features of cell death appear in the pre-follicular stage. 116 Narcosis with CO 2 could accelerate the general development of the virgin queen ovaries and prevented signals of PCD in ovaries, but it could not stop cell death if the virgin queen did not mate. Workers narcotized with CO 2 or coming from queenless colonies developed their ovaries, but in both the populations, worker ovaries presented signs of cell death when bees were 15 days old.…”

mentioning

confidence: 99%

Autophagy and its physiological relevance in arthropods: Current knowledge and perspectives

Malagoli¹,

Abdalla²,

Cao³

et al. 2010

Autophagy

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mentioning

confidence: 99%

Autophagy and its physiological relevance in arthropods: Current knowledge and perspectives

Malagoli¹,

Abdalla²,

Cao³

et al. 2010

Autophagy

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“…The ovary of the honey bee queen is polytrophic (multiple trophocytes per follicle) and meroistic (produce nutritive trophocytes and ova). Within each follicle, the trophocytes (nurse cells) (Figure 3A-4) are attached by intercellular bridges and connected to the oocyte by the trophic stalk (a narrow opening) (Figure 3A-3) which allows the transport of nutrients, RNA, and ribonucleoproteins from the trophocytes to the ovum during maturation (Figure 3A-1) as described in previous studies (Berger & da Cruz-Landim, 2009;Bili nski & Jaglarz, 1999;Cruz-Landim & Patr ıcio, 2010;Snodgrass, 1985;Tanaka et al, 2006). After transferring their cytoplasmic contents to the developing ovum, the trophocytes undergo programmed cell death (apoptosis) (Figure 3A-7) as found in earlier studies (Mpakou et al, 2006;Patr ıcio & Cruz-Landim, 2008).…”

Section: Ovariesmentioning

confidence: 73%

“…These studies focus on understanding both physiologic and pathologic processes in the ovary in response to various stimuli. For instance, ultrastructural evaluation of the ovary showed that delayed mating increases apoptotic and necrotic death of the ovarian germ line and somatic interstitial cells (Berger & da Cruz-Landim, 2009). Histology was used for more precise and reproducible estimation of ovariole numbers in honey bee queens (Jackson et al, 2011), which has since become one of the standard methods in honey bee research (Carreck et al, 2013).…”

Section: Use Of Light and Electron Microscopy In Honey Bee Researchmentioning

confidence: 99%

Histomorphological description of the reproductive system in mated honey bee queens

Kozii

Wood

Koziy

et al. 2021

Journal of Apicultural Research

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The accelerating decline of pollinator populations has become increasingly concerning in the last two decades. Honey bees are economically important pollinators and a model species to evaluate pollinator health. The decline in queen quality is one of the frequently reported causes associated with honey bee losses worldwide. Histopathology is an essential tool used for diagnostics and research in mammalian species and may provide insight into the histomorphological basis of a decline in queen quality. Thus, the purpose of this study was to summarize the previously described normal morphology of the entire reproductive tract of mated honey bee queens and to illustrate it by high quality photomicrographs. Accordingly, reproductive tracts of one-year old mated honey bee queens were processed for histology, serially sectioned, stained with hematoxylin and eosin, and used for the capture of high quality histological photomicrographs of the entire reproductive tract. This study illustrates the microscopic morphology of the queen reproductive tract which may facilitate further investigations of the cause and pathogenesis of the recent decline in reproductive fitness of honey bees using histopathology.

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“…This late germarium checkpoint is also found in nutritionally stressed Drosophila females (Drummond-Barbosa and Spradling 2001; Jenkins, Timmons et al 2013) and other insects (Velentzas, Nezis et al 2003; Uchida, Nishizuka et al 2004; Mpakou, Velentzas et al 2011). For honey bee workers, investing in oogenesis when there is no prospect of producing offspring is wasteful (Berger and da Cruz-Landim 2009). Therefore, it is advantageous for them to abort oocyte production before vitellogenesis, which is the most costly stage of oogenesis (Giorgi and Deri 1976; Drummond-Barbosa and Spradling 2001; Ronai, Vergoz et al 2016).…”

Section: Discussionmentioning

confidence: 99%

Regulation of oogenesis in the queen honey bee (Apis mellifera)

Aamidor

Harianto

et al. 2021

Preprint

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In the honey bee (Apis mellifera), queen and worker castes originate from identical genetic templates but develop into different phenotypes. Queens lay up to 2,000 eggs daily whereas workers are sterile in the queens presence. Periodically queens stop laying; during swarming, when resources are scarce in winter and when they are confined to a cage by beekeepers. We used confocal microscopy and gene expression assays to investigate the control of oogenesis in honey bee queen ovaries. We show that queens use different combination of checkpoints to regulate oogenesis compared to honey bee workers and other insect species. However, both queen and worker castes use the same programmed cell death pathways to terminate oocyte development at their caste-specific checkpoints. Our results also suggest that the termination of oogenesis in queens is driven by nutritional stress. Thus, queens may regulate oogenesis via the same regulatory pathways that were utilised by ancestral solitary species but have adjusted physiological checkpoints to suit their highly-derived life history.

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Ultrastructural analysis of the effect of mating delay on cell death in the ovaries of virgin honey bee (Apis mellifera L.) queens.

Cited by 9 publications

References 8 publications

Autophagy and its physiological relevance in arthropods: Current knowledge and perspectives

Autophagy and its physiological relevance in arthropods: Current knowledge and perspectives

Histomorphological description of the reproductive system in mated honey bee queens

Regulation of oogenesis in the queen honey bee (Apis mellifera)

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