Histomorphological description of the reproductive system in mated honey bee queens

Kozii, Ivanna V; Wood, Sarah C.; Koziy, Roman V; Simko, Elemir

doi:10.1080/00218839.2021.1900636

Cited by 3 publications

(3 citation statements)

References 58 publications

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“…To assist in microscopic evaluation, normal histology of insects was reviewed and a list of organs for examination was compiled (Table 1). 28,29,51 Inflammatory lesions were routinely stained with histochemical stains (Giemsa, Grocott’s methenamine silver [GMS], Gram, and/or Ziehl-Neelsen acid-fast stains) to assess for infectious agents.…”

Section: Methodsmentioning

confidence: 99%

Histological Findings in Captive Madagascar Hissing Cockroaches (Gromphadorhina portentosa) and a Literature Review

Monahan

Bogan

LaDouceur³

2023

Vet Pathol

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Madagascar hissing cockroaches (MHC, Gromphadorhina portentosa) are members of the Blaberidae (giant cockroaches) family of the Insecta class. They are native to the African island of Madagascar where they live within leaf litter on the rainforest floor. Due to their large size, relative tameness, and general easy keeping, they have become popular in classrooms, zoological collections, museums, research laboratories, and as private exotic pets; however, descriptions of diseases of MHC in the literature are rare. The objective of this study is to describe and characterize postmortem histological findings in 18 captive MHC from a single zoological collection. In this retrospective study, 18 (4 females and 14 males) adult MHC necropsies were submitted to Northwest ZooPath between 2016 and 2020 for evaluation. The main organs with histological lesions were chitinous gut (foregut and/or hindgut; n = 17), tracheae ( n = 15), fat body ( n = 14), ventriculus (midgut) ( n = 13), body wall ( n = 12), Malpighian tubules ( n = 12), and hemolymphatic sinuses ( n = 12). All animals had inflammatory lesions affecting various organs. Inflammatory lesions typically consisted of aggregates of hemocytes with variable amounts of melanization and/or encapsulation. Bacterial, fungal, and parasitic infections were common and variably associated with hemocytic inflammation. Many of these organisms may represent symbiotic organisms of the MHC that cause opportunistic infections. This study contributes to the current knowledge of pathological findings and disease response of MHC and reviews diseases reported in multiple cockroach species.

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Section: Methodsmentioning

confidence: 99%

Histological Findings in Captive Madagascar Hissing Cockroaches (Gromphadorhina portentosa) and a Literature Review

Monahan

Bogan

LaDouceur³

2023

Vet Pathol

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“…The proximal 1.5 mm of the embedded ovary (encompassing the entire terminal filament, germarium, and cranial vitellarium 14 ) was serially sectioned. Specifically, for each step, 30 consecutive 5-μm-thick sections were cut, of which the first 10 sections were collected on a glass slide, stained with H&E, and examined, whereas the following 20 sections were discarded.…”

Section: Methodsmentioning

confidence: 99%

“…The fixed ovaries were subjected to standard histological tissue processing and subsequently embedded in paraffin blocks in a vertical position so that the proximal portion of the ovary, including the terminal filament and germarium, could be sectioned first in cross sections. The proximal 1.5 mm of the embedded ovary (encompassing the entire terminal filament, germarium, and cranial vitellarium 14 ) was serially sectioned. Specifically, for each step, 30 consecutive 5-mm-thick sections were cut, of which the first 10 sections were collected on a glass slide, stained with H&E, and examined, whereas the following 20 sections were discarded.…”

Section: Histopathology and Morphometric Evaluationmentioning

confidence: 99%

Reproductive fitness of honey bee queens exposed to thiamethoxam during development

et al. 2021

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The productivity and survival of honey bee ( Apis mellifera) colonies depend on queen bee health. Colony-level neonicotinoid exposure has negative effects on reproductive fitness of honey bee queens. However, it is unclear if the observed effects are a direct outcome of neonicotinoid toxicity or result from suboptimal care of developing queens by exposed workers. The aim of this study was to evaluate larval survival, reproductive fitness, and histopathology of honey bee queens exposed to incremental doses (0, 5, 50 ng) of the neonicotinoid thiamethoxam (THI) applied directly to individual late larvae (7 days post-oviposition) of queens. The 5 ng dose represents a calculated high environmental level of exposure for honey bee queen larvae. Morphometric evaluation revealed that the total area of mandibular gland epithelium in queens exposed to 5 and 50 ng THI was reduced by 14% ( P = .12) and 25% ( P = .001), respectively. Decreased mandibular gland size may alter pheromone production, which could in part explain previously observed negative effects of THI on the reproductive fitness of queens. We also found that late larval exposure to THI reduced larval and pupal survival and decreased sperm viability in mated queens. These changes may interfere with queen development and reproductive longevity.

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Poor Air Quality Is Linked to Stress in Honeybees and Can Be Compounded by the Presence of Disease

Mayack,

Cook,

Niño

et al. 2023

Insects

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Climate change-related extreme weather events have manifested in the western United States as warmer and drier conditions with an increased risk of wildfires. Honeybees, essential for crop pollination in California, are at the center of these extreme weather events. We associated the maximum daily temperature and air quality index values with the performance of colonies placed in wildfire-prone areas and determined the impact of these abiotic stressors on gene expression and histopathology. Our results indicate that poor air quality was associated with higher maximum daily temperatures and a lower gene expression level of Prophenoloxidase (ProPO), which is tied to immune system strength; however, a higher gene expression level of Vitellogenin (Vg) is tied to oxidative stress. There was a positive relationship between Varroa mites and N. ceranae pathogen loads, and a negative correlation between Varroa mites and Heat Shock Protein 70 (HSP70) gene expression, suggesting the limited ability of mite-infested colonies to buffer against extreme temperatures. Histological analyses did not reveal overt signs of interaction between pathology and abiotic stressors, but N. ceranae infections were evident. Our study provides insights into interactions between abiotic stressors, their relation to common biotic stressors, and the expression of genes related to immunity and oxidative stress in bees.

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Histomorphological description of the reproductive system in mated honey bee queens

Cited by 3 publications

References 58 publications

Histological Findings in Captive Madagascar Hissing Cockroaches (Gromphadorhina portentosa) and a Literature Review

Histological Findings in Captive Madagascar Hissing Cockroaches (Gromphadorhina portentosa) and a Literature Review

Reproductive fitness of honey bee queens exposed to thiamethoxam during development

Poor Air Quality Is Linked to Stress in Honeybees and Can Be Compounded by the Presence of Disease

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