Ultrastructural Analysis of Human Endothelial Cells after Hypothermic Storage in Organ Preservation Solutions

Eberl, Thomas; Salvenmoser, Willi; Rieger, Gunde; Gorny, Irmina; Heiß, Veronika; Kumpitsch, Bettina; Gnaiger, Erich; Margreiter, Raimund

doi:10.1006/jsre.1998.5554

Cited by 30 publications

(19 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Une analyse ultrastructurale détaillée des cellules endothéliales humaines conservées pendant 6 heures en hypothermie dans des solutions de préservation a montré des lésions cellulaires comme la disparition des contacts intercellulaires, l'organisation des fibres de stress, la condensation nucléaire et la perte de l'intégrité mitochondriale. Il faut noter que les citernes endoplasmiques sont distendues, signe très suggestif de stress du RE [23]. Des données expérimentales obtenues chez des souris chez lesquelles on a provoqué un arrêt cardiaque, puis qu'on a ressuscitées, suggèrent que le stress du RE et la diminution de la traduction protéique jouent un rôle, mais les réponses à l'I/R sont différentes selon les organes.…”

Section: La Préservation D'organesunclassified

Implication du stress du réticulum endoplasmique en transplantation d’organe solide

et al. 2010

View full text Add to dashboard Cite

Section: La Préservation D'organesunclassified

Implication du stress du réticulum endoplasmique en transplantation d’organe solide

et al. 2010

View full text Add to dashboard Cite

“…52,53 Similarly, simulation of warm reperfusion of endothelial cells in vitro showed that UW solution was approximately 2 to 3 times more effective than EC solution. 52,53 Conversely, studies of isolated hepatocytes did not show the superiority of UW solution over EC solution. Comparison of EC and UW solutions with Krebs-Henseleit buffer showed that ion exchange levels were maintained in isolated rat hepatocytes stored in all media, suggesting that these solutions offer equal protection.…”

Section: Does Uw Solution Protect Only Endothelial Cells or Both Endomentioning

confidence: 99%

Role of hepatocytes and bile duct cells in preservation-reperfusion injury of liver grafts

Kukan

Haddad

2001

Liver Transplantation

View full text Add to dashboard Cite

In liver transplantation, it is currently hypothesized that nonparenchymal cell damage and/or activation is the major cause of preservation-related graft injury. Because parenchymal cells (hepatocytes) appear morphologically well preserved even after extended cold preservation, their injury after warm reperfusion is ascribed to the consequences of nonparenchymal cell damage and/or activation. However, accumulating evidence over the past decade indicated that the current hypothesis cannot fully explain preservation-related liver graft injury. We review data obtained in animal and human liver transplantation and isolated perfused animal livers, as well as isolated cell models to highlight growing evidence of the importance of hepatocyte disturbances in the pathogenesis of normal and fatty graft injury. Particular attention is given to preservation time-dependent decreases in high-energy adenine nucleotide levels in liver cells, a circumstance that (1) sensitizes hepatocytes to various stimuli and insults, (2) correlates well with graft function after liver transplantation, and (3) may also underlie the preservation timedependent increase in endothelial cell damage. We also review damage to bile duct cells, which is increasingly being recognized as important in the long-lasting phase of reperfusion injury. The role of hydrophobic bile salts in that context is particularly assessed. Finally, a number of avenues aimed at preserving hepatocyte and bile duct cell integrity are discussed in the context of liver transplantation therapy as a complement to reducing nonparenchymal cell damage and/or activation. (Liver Transpl 2001;7: 381-400.)A lthough the use of University of Wisconsin (UW) solution has permitted an increase in mean preservation time, the incidence of graft dysfunction still persists, 1,2 contributing significantly to a more than 20% per year mortality rate for patients in liver transplantation centers in the United States. 3 In addition, when storage time exceeds 10 to 12 hours, such late posttransplantation complications as biliary strictures occur in more than 25% of liver transplant recipients. [4][5][6] To prevent or minimize graft dysfunction and posttransplantation complications, it is essential to fully understand the importance of individual liver cell types in graft injury induced by cold storage and reperfusion. Insight into the cellular and molecular basis of these injuries will lead to the development of better intervention strategies, which is very important because graft dysfunction results in increased morbidity and enhances mortality rates. 7,8 Moreover, up to 30% of patients with graft dysfunction need retransplantation as early as within the first 3 months after transplantation, 2,7,8 which increases the demand on an already short pool of donor organs.According to morphological studies, cold preservation leads to injuries of nonparenchymal cells, whereas liver parenchymal cells appear well preserved. It is currently hypothesized that sinusoidal endothelial cell impairment, 9-11 activatio...

show abstract

“…In comparing UW and Euro-Collins solutions, several studies found no difference between the solutions (30, 31), while others found that UW is better at preserving viability (35,37). A functional assessment found Euro-Collins to be more damaging than UW after 24 h of storage (28).…”

Section: Many Of the Nuclei In The 24-h Euro-collins Stored Groupmentioning

confidence: 99%

“…These techniques are still in use (27), but it is appreciated that immediate function does not necessarily equate to long-term function, and that more subtle injury to the vasculature (3), in particular the endothelial cells, may result in structural changes within the vessels (7), leading to late graft failure (4). To address this issue, studies were designed to analyse the effects of various preservation solutions on tissue-cultured endothelial cells, with varying results (28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38). This technique plays a role in the assessment of preservation solutions, but is limited by the innate differences between cultured monolayers of cells and the same cells in there 'normal' environment, where they interact with extracellular matrix components and other constituent cells of the vessel walls (39).…”

Section: Introductionmentioning

confidence: 99%

Cold Storage Preservation and Warm Ischaemic Injury to Isolated Arterial Segments: Endothelial Cell Injury

Neil¹,

Lynch

Hardie

et al. 2002

American Journal of Transplantation

View full text Add to dashboard Cite

Injury to endothelial cells is thought to be important to the development of the vascular lesion of chronic rejection. It was the aim of this study to develop a semiquantitative method to assess endothelial injury in arterial grafts and to document the injury produced by cold storage preservation and additional warm ischaemia. Twelve-and 24-h cold preservation of rat aortic segments, together with an additional 1 h of warm ischaemia, were assessed. Electron micrographs of representative endothelial cells were scored for cytoplasmic, nuclear and mitochondrial injury.

show abstract

Ultrastructural Analysis of Human Endothelial Cells after Hypothermic Storage in Organ Preservation Solutions

Cited by 30 publications

References 24 publications

Implication du stress du réticulum endoplasmique en transplantation d’organe solide

Implication du stress du réticulum endoplasmique en transplantation d’organe solide

Role of hepatocytes and bile duct cells in preservation-reperfusion injury of liver grafts

Cold Storage Preservation and Warm Ischaemic Injury to Isolated Arterial Segments: Endothelial Cell Injury

Contact Info

Product

Resources

About