Cold Storage Preservation and Warm Ischaemic Injury to Isolated Arterial Segments: Endothelial Cell Injury

Abstract

Injury to endothelial cells is thought to be important to the development of the vascular lesion of chronic rejection. It was the aim of this study to develop a semiquantitative method to assess endothelial injury in arterial grafts and to document the injury produced by cold storage preservation and additional warm ischaemia. Twelve-and 24-h cold preservation of rat aortic segments, together with an additional 1 h of warm ischaemia, were assessed. Electron micrographs of representative endothelial cells were scored for cytoplasmic, nuclear and mitochondrial injury.

Improvement of the cold storage of blood vessels with a vascular preservation solution. Study in porcine aortic segments

“…Cold storage of vascular endothelial cells has been shown to induce increased adhesion of platelets, 36 and storage of vessels has been shown to disrupt the endothelial monolayer. 37,38 Both might lead to thrombosis and graft occlusion, which could result in the loss of the organ/tissue supplied by the grafted vessel. 39,40 In solution 8 with 0.1 mmol/L deferoxamine ϩ 20 mol/L LK 614, the platelet adherence was significantly reduced compared with HTK solution, indicating lower thrombogenicity.…”

Improved vessel preservation after 4 days of cold storage: Experimental study in rat arteries

“…A recent study 15 has addressed endothelial structural changes of rat abdominal aorta after 24 hours of storage in University of Wisconsin (UW), Collins, and Euro-Collins solutions. Endothelial cell structure was maintained after 24 hours only in UW-solution.…”

“…13,14 A 24-48 hour period of successful endothelium and microvessel preservation during cold storage has been reported. [15][16][17] It seems highly desirable to extend this period and improve the availability of donor tissues. A recent experimental study reported substantial progress with respect to long-term storage of pig aortic segments over 3 weeks.…”

Strategies to optimize kidney recovery and preservation in transplantation: specific aspects in pediatric transplantation

“…At the cellular level, the mitochondria and the endoplasmic reticulum are the principal organelles affected [36], with the result that cells are constrained in adapting their biochemical programs. Indeed, ischemia triggers a switch in energy production by activating glycolysis in anaerobic conditions, leading to a sudden fall in ATP concentration due to a lower yield of ATP production compared to oxidative phosphorylation.…”