Ultrastructural alterations of the striated muscle cells in diabetes mellitus

Cytoarchitecture of muscle in a genetic model of murine diabetes

“…The evidence of myofiber degeneration -noted in this study as well as in those of others (Awad and Kottke, 1970;Campeanu et al, 1971;Picard and De la Monte, 1987) may be due either to changes in insulin levels (Jefferson et al, 1974;Pain and Garlick, 1974;Goldberg, 1979;Flaim et al, 1980;Tischler, 198l>, which result in protein breakdown, or to focal and intermittent areas of ischemia (Awad and Kottke, 1970;Vracko, 1970). The presence of small myofibers with centrally placed nuclei noted in this study may indicate a regenerative attempt by the muscle to replace the dying myofibers.…”

“…Excess lipid accumulations are one feature in diabetic muscle that may be a response to metabolic changes. The lipid accumulations have been noted both in animal models of diabetes (Chao et al, 1976) and in human diabetic muscle (Naccarato et al, 1970;Campeanu et al, 1971). The increased lipid content of the myofibers observed in this study may be due to the increased number of type 2a myofibers in the diabetic muscle (Klueber et al, 1988).…”

“…Springs Instruments, Ohio). Blood sugars above 250 mg/ for a few studies that examined changes in skeletal tocin (Armstrong et al, 1975;Chao et al, 1976) and Under methoxyflurane (Metofane, Pitman-Moore, human muscle biopsies (Hamilton et al, 1968;Awad Washington Crossing, NJ) anesthetic, the extensor digiand Kottke, 1970;Naccarato et al, 1970;Vracko, 1970; torum longus muscles (EDL) for both the control and the Campeanu et al, 1971;Picard and De la Monte, 1987). diabetic animals were exposed.…”

Ultrastructural, histochemical, and morphometric analysis of skeletal muscle in a murine model of type I diabetes

“…The swollen abnormal mitochondria observed here have been associated with necrosis of myofibers (Carpenter and Karpati, 1984). This disorganization of the cristae has been also associated with accumulation of intramitochondrial granules in the STZ model (Campeanu et al, 1971;Chao et al, 1976) as well as in a genetic model of the disease (Feczko and . In mitochondria1 myopathies, the mitochondria are enlarged with an abnormal appearance, increased in number, and localized around the periphery of the myofiber (Astrom and Adams, 1988).…”

“…The deposition of lipid stores within myofibers has been associated with muscle in diabetes (Naccarato et al, 1970;Campeanu et al, 1971;Chao et al, 1976;Fig. 8. A typical STZ myofiber with focal necrosis and dissolution of myofilaments around the nucleus.…”