Ultrasound Combined with Microbubbles Enhances the Effects of Methylprednisolone in Lipopolysaccharide-Induced Human Mesangial Cells

Tian, Tian; Chen, Li Na; Fu, Rong; Xu, Shan; Ai, Hong; Wang, Bing; Zhang, Jian; Yan, Ruo; Chai, Zhonglin; Cooper, Mark E.; Ren, Shu

doi:10.1124/jpet.117.246223

Cited by 4 publications

(1 citation statement)

References 43 publications

(41 reference statements)

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“…Both of our previous and recent studies proved that LPS induced HMC proliferation (Sun et al, 2018; Wu et al, 2018). To confirm the role of paeoniflorin in protecting mesangial cells in vitro , HMCs were incubated with 30 μg/ml LPS and different dose of paeoniflorin for 24 h. As shown in Figure 5A , MTT assay results showed that LPS significantly promoted cell vitality of HMCs ( P < 0.05), while paeoniflorin (from 5μM to 40μM) inhibited the growth of HMCs ( P < 0.01).…”

Section: Resultsmentioning

confidence: 65%

Paeoniflorin Inhibits Mesangial Cell Proliferation and Inflammatory Response in Rats With Mesangial Proliferative Glomerulonephritis Through PI3K/AKT/GSK-3β Pathway

et al. 2019

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Mesangial proliferative glomerulonephritis (MPGN) is the most common type of chronic kidney disease in China, characterized by mesangial cell proliferation and inflammatory response. Paeoniflorin, an effective composition extracted from Radix Paeoniae Alba, has been used for various kinds of kidney diseases. However, there are no studies reporting the effects of paeoniflorin on MPGN. The present study aims to investigate whether paeoniflorin plays a role in MPGN and confirm the underlying molecular mechanisms. Our results manifested that paeoniflorin strongly restrained 24 h urinary protein and promoted renal function and dyslipidemia in a MPGN rat model. Moreover, paeoniflorin attenuated mesangial cell proliferation and inflammation both in MPGN rats and human mesangial cells (HMCs) treated with lipopolysaccharide (LPS). In detail, paeoniflorin decreased the number of mesangial cells and expressions of proliferation marker Ki67 in MPGN rats. Paeoniflorin also inhibited HMC proliferation and blocked cell cycle progression. In addition, the contents of inflammatory factors and the expressions of macrophage marker iNOS were decreased after paeoniflorin treatment. Furthermore, we found that the protective effect of paeoniflorin was accompanied by a strong inhibition of the phosphatidylinositol 3-kinase (PI3K)/AKT/glycogen synthase kinase (GSK)-3β pathway. Paeoniflorin enhanced the inhibitory effect of PI3K inhibitor LY294002 and suppressed the activated effect of PI3K agonist insulin-like growth factor 1 (IGF-1) on PI3K/AKT/GSK-3β pathway. In conclusion, these results demonstrated that paeoniflorin ameliorates MPGN by inhibiting mesangial cell proliferation and inflammatory response through the PI3K/AKT/GSK-3β pathway.

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Section: Resultsmentioning

confidence: 65%