Ultrasound assisted modulation of yeast growth and inactivation kinetics

Soro, Arturo B.; Oliveira, Márcia; O’Donnell, Colm P.; Tiwari, Brijesh K.

doi:10.1016/j.ultsonch.2021.105819

Cited by 12 publications

(8 citation statements)

References 34 publications

(64 reference statements)

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“…2020; Soro et al . 2021) and preliminary analysis (data not shown) which indicated that yeast envelope disarrangement (cellular breakage) strongly occurs at ultrasound densities higher than 3 kW l −1 .…”

Section: Resultsmentioning

confidence: 91%

“…Here, the UABE protocol enabled the capture of nonencapsulated hydrophobes (above 58 and 76% for curcumin and fisetin, respectively) as well as promoted the incorporation yield of the bioparticles by adding nonloaded yeasts between each ES. Furthermore, the AEDs were chosen based on previous works (Tao et al 2019;Pagnossa et al 2020;Soro et al 2021) and preliminary analysis (data not shown) which indicated that yeast envelope disarrangement (cellular breakage) strongly occurs at ultrasound densities higher than 3 kW l −1 .…”

Section: General Features Of the Uabe Processmentioning

confidence: 99%

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Ultrasound-assisted encapsulation of curcumin and fisetin into Saccharomyces cerevisiae cells: a multistage batch process protocol

Andrade

Hoskin

Pedrini

2022

Letters in Applied Microbiology

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Some of the challenges of yeast encapsulation protocols are low phytochemical internalization rates and limited intracellular compartments of yeasts. This study uses an ultrasound‐assisted batch encapsulation (UABE) protocol to optimize the encapsulation of curcumin and fisetin by recovering nonencapsulated biomaterial and further incorporating it into nonloaded yeasts in three encapsulation stages (1ES, 2ES and 3ES). The effect of selected acoustic energies (166·7 and 333·3 W l−1) on the encapsulation efficiency (EE), yield (EY) and antioxidant activity retention were evaluated, and then, compared with a control process (without ultrasound treatment). Compared to the control, enhanced EEs were achieved for both curcumin (10·9% control to 58·5% UABE) and fisetin (18·6% control to 76·6% UABE) after 3ES and the use of 333·3 W l−1. Similarly, the yeast maximum loading capacity was improved from 6·6 to 13·4 mg g−1 for curcumin and from 11·1 to 26·4 mg g−1 for fisetin after UABE protocol. The antioxidant activity of produced biocapsules was positively correlated with the bioactive‐loaded content of yeasts when ultrasound treatment was applied. Overall, results from this study provide valuable information regarding UABE processes, and moreover, bring new and creative perspectives for ultrasound technology in the food industry.

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Section: Resultsmentioning

confidence: 91%

Section: General Features Of the Uabe Processmentioning

confidence: 99%

Ultrasound-assisted encapsulation of curcumin and fisetin into Saccharomyces cerevisiae cells: a multistage batch process protocol

Andrade

Hoskin

Pedrini

2022

Letters in Applied Microbiology

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“…The modified Gompertz model (Equation (1)) was used to evaluate the effect of temperature on the shape of each isolate growth curve and determine the maximum growth rate (μ max ) and the lag phase duration [ 54 ]. The model parameters were determined by fitting the growth curves to the Gompertz equation using the Levenberg–Marquardt nonlinear least squares method programmed in the MATLAB software (MathWorks, Inc., Natick, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

Genotypic and Phenotypic Diversity of Kluyveromyces marxianus Isolates Obtained from the Elaboration Process of Two Traditional Mexican Alcoholic Beverages Derived from Agave: Pulque and Henequen (Agave fourcroydes) Mezcal

Lappe-Oliveras,

Avitia,

Sánchez-Robledo

et al. 2023

JoF

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Seven Kluyveromyces marxianus isolates from the elaboration process of pulque and henequen mezcal were characterized. The isolates were identified based on the sequences of the D1/D2 domain of the 26S rRNA gene and the internal transcribed spacer (ITS-5.8S) region. Genetic differences were found between pulque and henequen mezcal isolates and within henequen mezcal isolates, as shown by different branching patterns in the ITS-5.8S phylogenetic tree and (GTG)5 microsatellite profiles, suggesting that the substrate and process selective conditions may give rise to different K. marxianus populations. All the isolates fermented and assimilated inulin and lactose and some henequen isolates could also assimilate xylose and cellobiose. Henequen isolates were more thermotolerant than pulque ones, which, in contrast, presented more tolerance to the cell wall-disturbing agent calcofluor white (CFW), suggesting that they had different cell wall structures. Additionally, depending on their origin, the isolates presented different maximum specific growth rate (µmax) patterns at different temperatures. Concerning tolerance to stress factors relevant for lignocellulosic hydrolysates fermentation, their tolerance limits were lower at 42 than 30 °C, except for glucose and furfural. Pulque isolates were less tolerant to ethanol, NaCl, and Cd. Finally, all the isolates could produce ethanol by simultaneous saccharification and fermentation (SSF) of a corncob hydrolysate under laboratory conditions at 42 °C.

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“…Furthermore, fitted growth curves were evaluated qualitatively using the regression coefficient R 2 as a measures of goodness-of-fit. Growth model fitting and statistical evaluation of fitted models were in accordance with the equations used in a study by Soro et al 20 .…”

Section: Methodsmentioning

confidence: 99%

Examining the impact of zinc on horizontal gene transfer in Enterobacterales

Ekhlas

Soro

Leonard

et al. 2022

Sci Rep

Self Cite

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Antimicrobial resistance is one of the main international health concerns for humans, animals, and the environment, and substantial efforts have focused on reducing its development and spread. While there is evidence for correlations between antimicrobial usage and antimicrobial resistance development, specific information on the effect of heavy metal/antimicrobial usage on bacterial conjugation is more limited. The aim of this study was to investigate the effects of zinc and antimicrobials in different concentrations on horizontal gene transfer of an ampicillin resistance gene, using a multi-drug resistant Escherichia coli donor strain and three different Salmonella enterica serovars as recipient strains. Differences in conjugation frequencies for the different Salmonella recipients were observed, independent of the presence of zinc or the antimicrobials. Selective pressure on the recipient strains, in the form of ampicillin, resulted in a decrease in conjugation frequencies, while, the presence of rifampicin resulted in increases. Zinc exposure affected conjugation frequencies of only one of the three recipient strains, thus the effect of zinc on conjugation frequencies seemed to be concentration and strain dependent. Furthermore, differences in growth rates due to plasmid carriage were observed for one of the Salmonella strains.

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Ultrasound assisted modulation of yeast growth and inactivation kinetics

Cited by 12 publications

References 34 publications

Ultrasound-assisted encapsulation of curcumin and fisetin into Saccharomyces cerevisiae cells: a multistage batch process protocol

Ultrasound-assisted encapsulation of curcumin and fisetin into Saccharomyces cerevisiae cells: a multistage batch process protocol

Genotypic and Phenotypic Diversity of Kluyveromyces marxianus Isolates Obtained from the Elaboration Process of Two Traditional Mexican Alcoholic Beverages Derived from Agave: Pulque and Henequen (Agave fourcroydes) Mezcal

Examining the impact of zinc on horizontal gene transfer in Enterobacterales

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