Ultrasound-Assisted Extraction, Antioxidant and Anticancer Activities of the Polysaccharides from Rhynchosia minima Root

Abstract

Box-Behnken design (BBD), one of the most common response surface methodology (RSM) methods, was used to optimize the experimental conditions for ultrasound-assisted extraction of polysaccharides from Rhynchosia minima root (PRM). The antioxidant abilities and anticancer activity of purified polysaccharide fractions were also measured. The results showed that optimal extraction parameters were as follows: ultrasound exposure time, 21 min; ratio of water to material, 46 mL/g; ultrasound extraction temperature, 63˝C. Under these conditions, the maximum yield of PRM was 16.95%˘0.07%. Furthermore, the main monosaccharides of purified fractions were Ara and Gal. PRM3 and PRM5 exhibited remarkable DPPH radical scavenging activities and reducing power in vitro. PRM3 showed strong inhibitory activities on the growth of MCF-7 cells in vitro. The above results indicate that polysaccharides from R. minima root have the potential to be developed as natural antioxidants and anticancer ingredients for the food and pharmaceutical industries.

Optimization of Ultrasound-Assisted Extraction of Flavonoids from Celastrus hindsii Leaves Using Response Surface Methodology and Evaluation of Their Antioxidant and Antitumor Activities

“…where Y is the TFC (mg QE/g); X 1 , X 2 , X 3 , and X 4 are the ultrasonic power (W), extraction temperature (°C), extraction time (min), and ethanol concentration (%), respectively; β 0 is the intercept coefficient, β 1 -β 4 are the linear coefficients, β 11 , β 22 , β 33 , and β 44 are the squared coefficients, and 24 , and β 34 are the interaction coefficients.…”

“…In recent years, the ultrasound-assisted process has gained increased interest in the phytochemical extraction due to its rapid and high extraction efficiency [21,24]. is method employs ultrasonication, which is ultrasonic waves, to disrupt a cell wall through inducing cavitation in the solvent [21].…”

“…is method enhances the mass transfer and solvent penetration into the plant materials, which promote the release of soluble compounds from the plant cell into the extracting solvent [21,25]. Consequently, this method shortens the extraction time and enhances the extraction efficiency [24]. Remarkably, the ultrasoundassisted extraction process proceeds at low temperatures, thus reducing damage to the molecular and structural properties of extracted compounds [24].…”

“…Consequently, this method shortens the extraction time and enhances the extraction efficiency [24]. Remarkably, the ultrasoundassisted extraction process proceeds at low temperatures, thus reducing damage to the molecular and structural properties of extracted compounds [24]. In addition, this process has been proved to be an eco-friendly and economically viable process and easier to scale up for industrial applications as compared to microwave-assisted extraction and supercritical extraction [21,25].…”

“…In addition, this process has been proved to be an eco-friendly and economically viable process and easier to scale up for industrial applications as compared to microwave-assisted extraction and supercritical extraction [21,25]. Ultrasound-assisted extraction has been successfully applied in the extraction of several bioactive compounds such as polysaccharides [21,24] and total phenolic compounds [15,17]. However, no study has investigated the use of the ultrasound-assisted technique for the extraction of flavonoids from C. hindsii.…”