Optimization of Ultrasound-Assisted Extraction of Flavonoids from Celastrus hindsii Leaves Using Response Surface Methodology and Evaluation of Their Antioxidant and Antitumor Activities

Abstract

Celastrus hindsii is a potential source of flavonoids with biological activities. This study aimed to develop an ultrasound-assisted technique for extracting flavonoids from leaves of C. hindsii. Response surface methodology was employed to optimize the extraction conditions for maximizing the total flavonoid content (TFC). A maximum TFC of 23.6 mg QE/g was obtained under the extraction conditions of ultrasonic power of 130 W, extraction temperature of 40°C, extraction time of 29 min, and ethanol concentration of 65%. The flavonoid-rich extracts were then studied for their antioxidant and anticancer activities. The results showed that the C. hindsii leaf extract exhibited potent radical scavenging activities against DPPH (IC50 of 164.85 μg/mL) and ABTS (IC50 of 89.05 μg/mL). The extract also significantly inhibited the growth of 3 cancer cell lines MCF7, A549, and HeLa with the IC50 values of 88.1 μg/mL, 120.4 μg/mL, and 118.4 μg/mL, respectively. Notably, the extract had no cytotoxicity effect on HK2 normal kidney cell line. This study suggests that flavonoid-rich extract is a promising antioxidant and anticancer agent and that ultrasound-assisted extraction is an efficient method for extracting flavonoids from C. hindsii leaves.

Ultrasound Assisted Extraction of Phenolic Compounds from a Jujube By-Product with Valuable Bioactivities

“…This proved in several studies to develop a lot of diseases. In order to protect our human body from these diseases, the antioxidants are showed to be effective for neutralization of free radicals [14]. However, to determine whether the UAE extraction process impact the biological functions, the antioxidant effects of jujube seeds extracts were examined and evaluated by DPPH radical scavenger and FRAP model ( Table 3).…”

“…Cancer, known as the disease of the century, is the primary cause of death in the globe after cardiovascular diseases [14,15]. One of the means of intervention is the inhibition of the proliferation cancer cell.…”

“…Among these, one of the simplified method in manipulation is ultrasound-assisted extraction (UAE) that was very used on extraction of phenolic compounds from different materials due to its high reproducibility and very appreciated for its reduction in solvent consumption [30,31]. Response surface methodology (RSM) is used as an effective statistical method for optimizing complex processes that reduce the number of experimental trials which is very used to evaluate interactions between multiple factors and the response variables that influence the results [14,32].…”

Comparison between maceration and ultrasound-assisted extraction of white bracts with flowers of Bougainvillea spectabilis Willd.

“…To obtain these compounds, the extraction process is basically the most important step of the analysis, which happens through the action of a solvent in direct contact with the plant cell structure (MEREGALLI et al, 2020;PHAM et al, 2020). Aspects such as type of solvent, temperature, pH of the medium, and time of extraction directly interfere in the process of obtaining bioactive compounds, and as a result can alter their biological activities (NADEEM et al, 2018).…”

Bioactive Compounds, Antioxidants, and Health Benefits of Sweet Potato Leaves

“…The search for dietary sources with potent biological activities has increasingly attracted considerable attention [94][95][96][97]. There is a great deal of interest in using potent dietary antioxidants in foods and pharmaceuticals to prevent oxidative reactions and chronic degenerative diseases [98][99][100]. SPL are a good source of nutrients, enhancing dietary protein, amino acid intake, and growth performance [66,67,101].…”