Ultrasound-assisted dispersive extraction for the high pressure liquid chromatographic determination of tetracyclines residues in milk with diode array detection

Karageorgou, Eftichia G.; Armeni, Marina; Moschou, Ioulia Ch.; Samanidou, Victoria F.

doi:10.1016/j.foodchem.2013.11.008

Cited by 58 publications

(26 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The performance characteristics of the method are comparable with those obtained by other methods, including those in which dispersive solid-phase microextraction [6] and ultrasound-assisted dispersive extraction [8] were applied for the determination of tetracycline residues in milk samples.…”

Section: Discussionsupporting

confidence: 58%

“…Many sample preparation strategies have been developed for the isolation and clean-up of tetracycline residues in milk throughout the years, such as solid phase extraction (SPE) with various types of sorbents [4,5], solid phase microextraction (SPME) [6], hollow-fiber liquid phase microextraction (HF-LPME) [7] and ultrasound assisted dispersive extraction [8].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Development of a High Pressure Liquid Chromatography with Diode Array Detection Method for the Determination of Four Tetracycline Residues in Milk by Using QuEChERS Dispersive Extraction

2019

Self Cite

View full text Add to dashboard Cite

QuEChERS (quick, easy, cheap, effective, rugged and safe) dispersive extraction was applied for the extraction of tetracyclines (oxytetracycline, tetracycline, chlorotetracycline and doxycycline) from milk. Target analytes were determined by an accurate and sensitive chromatographic analytical method, which was validated in terms of 2002/6572/EC decision. The analytes were separated on an Orbit 100C 4 (5 µm, 250 × 4.0 mm) analytical column under a gradient mobile phase composed of a mixture of 0.01 M oxalic acid, 10 −4 M Na 2 EDTA and acetonitrile. For the extraction of isolated compounds from sorbent, a methanol and 0.01 M oxalic acid mixture (1:1 v/v) was used, leading to relative recovery rates from 83.07% to 106.3% at concentration levels in the range 100-200 µg/kg. The within-laboratory reproducibility, expressed as a relative standard deviation, was <15.5%. Decision limits ranged between 100.3 µg/kg and 105.6 µg/kg and the detection capability varied between 100.6 µg/kg and 109.7 µg/kg. Ruggedness was evaluated by following the Youden approach, in terms of milk mass, sorbent mass, centrifugation time, vortex time, type and volume of organic solvents and evaporation temperature.Separations 2019, 6, 21 2 of 9 solid-phase extraction (D-SPE) clean-up step. This process was firstly developed for the extraction of pesticides from different matrices, such as soil [10], sugarcane juice [11], honey [12], wheat grains, flour and bran [13], but also for the extraction of drugs and phenols from soil [14-16] and veterinary drug residues from foods of animal origin [17][18][19].Although a number of methods [20,21] can be found in the literature employing mass spectrometric detection for confirmation purposes, this instrumentation is not always available in analytical laboratories. The aim of this study was to develop a simple and quick HPLC-DAD method for the determination of four tetracyclines (oxytetracycline, tetracycline, chlorotetracycline and doxycycline) in milk using QuEChERS dispersive extraction. The method was validated according to the European Union Decision 2002/657/EC with regards to selectivity, linearity, accuracy, precision, sensitivity and ruggedness by applying the Youden approach [22].

show abstract

Section: Discussionsupporting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Development of a High Pressure Liquid Chromatography with Diode Array Detection Method for the Determination of Four Tetracycline Residues in Milk by Using QuEChERS Dispersive Extraction

2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…The target analytes are extracted from the cartridge with a suit able eluant. The MSPD method was used for the sep aration of tetracycline antibiotics from milk [55,56], meat [26,57], fish [58], and eggs [59]. Yu et al [57] compared the effectiveness of eluants.…”

Section: Sample Preparationmentioning

confidence: 99%

“…Honey HPLC [34], [35], [37], [69], [73] FS [120] IC [36], [38], [135][136][137] CL [116] CL [116] FIA [33] FIA [33] VA [122], [123] FS [39] ISE [91], [121] Milk HPLC [55], [60], [71][72][73][74], [80], [81], [86], [138], [139] Blood and plasma CZE [75] CZE [56], [75], [76] CEC [128] IC [20], [135], [136] Various hydrophobic sorbents with grafted alkyl radicals, as a rule, C18, are used as stationary phases in reversed phase HPLC. Table 3 summarizes the exam ples of commercially available stationary phases used for the determination of tetracyclines in food prod ucts, feed, and environmental samples.…”

Section: Ms-dart [157]mentioning

confidence: 99%

“…Photometric UV [23,51,53,60,73,74,80,81,89,113,124,134], diode array [15,19,22,29,37,55,68,72,84,86,138,139], and fluorescence [34,58,71,83,93,94] detectors are used for the detection of tetracy cline antibiotics. The number of works regarding the determination of tetracycline antibiotics by the HPLC with a mass spectrometric detector noticeably increased [15,21,27,32,35,69,100,105,106,112,114].…”

Section: Ms-dart [157]mentioning

confidence: 99%

See 1 more Smart Citation

Methods for the separation, preconcentration, and determination of tetracycline antibiotics

2015

View full text Add to dashboard Cite

The data published in the last five years concerning the determination of tetracycline antibiotics in different samples are generalized. Sample preparation procedures for real samples, including liquid extrac tion from solid matrices, pressurized liquid extraction, matrix solid phase dispersion, QuEChERS method, and solid phase extraction, are compared. The following important determination techniques are discussed: chromatographic, electrophoretic, spectroscopic, and electrochemical. The determination of the above anti biotics in food products, feed, environmental samples, pharmaceuticals, and biological fluids is exemplified.

show abstract

Main uses of Microwaves and Ultrasounds in Analytical Extraction Schemes: an Overview

Pacheco‐Fernández

González‐Hernández

Rocío‐Bautista

et al. 2015

Analytical Separation Science

View full text Add to dashboard Cite

The utilization of microwaves and/or ultrasounds in analytical extraction procedures is a common practice nowadays. Both extraction approaches can be classified as routine/conventional tools. This chapter intends to give an overview of main applications of microwaves and/or ultrasounds as neat analytical approaches, assisting other extraction methods, or combined with other procedures in a hybrid strategy. Advances in the instrumentation together with the utilization of novel solvents will be also highlighted. The chapter will also show most common applications as a function of the nature of the analyte or the kind of sample analyzed.

show abstract

Ultrasound-assisted dispersive extraction for the high pressure liquid chromatographic determination of tetracyclines residues in milk with diode array detection

Cited by 58 publications

References 19 publications

Development of a High Pressure Liquid Chromatography with Diode Array Detection Method for the Determination of Four Tetracycline Residues in Milk by Using QuEChERS Dispersive Extraction

Development of a High Pressure Liquid Chromatography with Diode Array Detection Method for the Determination of Four Tetracycline Residues in Milk by Using QuEChERS Dispersive Extraction

Methods for the separation, preconcentration, and determination of tetracycline antibiotics

Main uses of Microwaves and Ultrasounds in Analytical Extraction Schemes: an Overview

Contact Info

Product

Resources

About