Ultrasound-assisted deep eutectic solvents extraction of glabridin and isoliquiritigenin from Glycyrrhiza glabra: Optimization, extraction mechanism and in vitro bioactivities

Chen, Xing; Cui, Weihong; Zhang, Yue; Zou, Xin-Shu; Hao, Jingyou; Zheng, Sidi; Wang, Tingting; Wang, Xiaozhen; Wu, Tong; Liu, Yanyan; Chen, Xueying; Yuan, Shuguang; Zhang, Zhiyun; Li, Yanhua

doi:10.1016/j.ultsonch.2022.105946

Cited by 44 publications

(30 citation statements)

References 49 publications

(55 reference statements)

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“…Interestingly, the highest yield of TAC and the lowest yield of cyanidin-3- O -glucoside were recorded for the same NaDES 1 solvent containing lactic acid. In fact, NaDES of low pH and viscosity, such as NaDES 1, favor the extraction of polyphenolic compounds [ 10 ]. The yield discrepancy between TAC and individual cyanidin-3- O -glucosides can be explained by the acid hydrolysis of anthocyanin heterosides on sugar and aglycone units that can occur at elevated temperatures.…”

Section: Resultsmentioning

confidence: 99%

“…This is probably due to the difference in viscosity among NaDES. Choline chloride-based NaDES with citric, malic, and tartaric acid usually show a higher viscosity than lactic acid [ 10 ]. The higher viscosity of NaDES is usually correlated with weaker extractability but improved stabilization properties.…”

Section: Resultsmentioning

confidence: 99%

“…In fact, NaDES are a mixture of primary plant metabolites, such as sugars (glucose, fructose, sucrose, trehalose, maltose), sugar alcohols (sorbitol, glycerol, 1,2-propanediol, xylitol), organic acids (lactic, citric, maleic, malic, oxalic, ascorbic), amino acids (proline, glycine, alanine), and amines (betaine, choline) [ 9 ]. The NaDES components play the role of hydrogen bond donors or acceptors and can build an intermolecular hydrogen bond network resulting in the lowering of the mixture melting point and forming the viscous liquid systems of outstanding solubilizing properties [ 10 ]. Other favorable properties of the NaDES include simple and inexpensive synthesis, sustainability, non-flammability, non-volatility, biodegradability, and biocompatibility [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

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Ultrasound-Assisted Natural Deep Eutectic Solvents Extraction of Bilberry Anthocyanins: Optimization, Bioactivities, and Storage Stability

Jovanović

Krgović

Živković

et al. 2022

Plants

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Bilberry fruits (Vaccinium myrtillus L.) are one of the richest natural sources of anthocyanins and are widely used due to their pharmacological and nutritional properties. To ensure their maximum application potential, it is necessary to overcome the limitations of conventional extraction solvents and techniques. This study aimed to develop a green method for bilberry anthocyanin extraction using natural deep eutectic solvents (NaDES) integrated with ultrasound-assisted extraction (UAE) in order to define extraction conditions that will prevent decomposition of the anthocyanins or the loss of bioactivity. After a screening of ten different NaDES, choline chloride:sorbitol (1:1) was selected as the most effective. Furthermore, the influence analysis and optimization of the NaDES–UAE extraction conditions were carried out employing response surface methodology. The optimal conditions were found to be an extraction time of 37.63 min, a temperature of 48.38 °C, and 34.79% (w/w) water in NaDES. The extraction yields of target compounds under optimized extraction conditions were 0.27 mg/g DW of cyanidin-3-O-glucoside and 2.12 mg CGE/g DW of TAC. The obtained optimized extract showed promising radical scavenging and antimicrobial activity. A stability study with the optimized extract revealed that refrigerated storage at 4 °C in the dark provided the best anthocyanins preservation. Overall, the developed NaDES-UAE method showed promising application potential and can be considered as a high-efficiency green alternative to conventional anthocyanins extraction methods, enabling the preservation of active ingredients and the bioactivity of extracts.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ultrasound-Assisted Natural Deep Eutectic Solvents Extraction of Bilberry Anthocyanins: Optimization, Bioactivities, and Storage Stability

Jovanović

Krgović

Živković

et al. 2022

Plants

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“…The conventional extraction methods, such as maceration, digestion, percolation, Soxhlet extraction, have been the most utilized techniques throughout the decades. However, they have some disadvantages, such as long duration of the process, low efficiency, requirement of large volumes of the extraction solvents, the unsuitability of some of the methods for thermolabile substances [ 12 , 13 , 14 , 15 ]. Therefore, some new, unconventional extraction techniques are being considered and becoming more and more popular, such as ultrasound-assisted extraction, microwave-assisted extraction, supercritical CO 2 extraction, etc.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, some new, unconventional extraction techniques are being considered and becoming more and more popular, such as ultrasound-assisted extraction, microwave-assisted extraction, supercritical CO 2 extraction, etc. [ 15 ]. These methods offer the possibility of higher extraction yields, shorter extraction time and environmentally friendly approaches, as well as reduced consumption of extraction solvents and less generation of toxic residues [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Conventional vs. Green Extraction Using Natural Deep Eutectic Solvents—Differences in the Composition of Soluble Unbound Phenolic Compounds and Antioxidant Activity

et al. 2022

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The aim of this study was to investigate the effect of the use of green solvents, natural deep eutectic solvents (NaDES), in comparison with conventional solvents on the extraction of free unbound phenolic compounds and the antioxidant activity of extracts of dried bilberry fruit, bilberry leaves and green tea leaves. After preparation of the extracts via ultrasound-assisted extraction using NaDES and conventional solvents (water and ethanol), spectrophotometric determination of total phenolic and flavonoid content, HPLC analysis of extracted polyphenols and antioxidant determination using FRAP, DPPH and ABTS assays were conducted. The results showed that NaDES have a great potential as agents for the extraction of phenolic compounds with potent antioxidant activity; the highest values of phenolic content and antioxidant activity were detected in the samples obtained by extraction using the NaDES combination betaine + urea. The bilberry leaves exhibited the highest flavonoid content among all extracts and turned out to be more active than bilberry fruits, to which they are often just a by-product during processing. The most active extract of all was the betaine-urea green tea leaves extract. Further research into the most active NaDES extracts should be performed.

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Advances of responsive deep eutectic solvents and application in extraction and separation of bioactive compounds

Zhang

Gul

et al. 2023

J of Separation Science

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In recent years, it has been found that changing ambient conditions (CO2/N2, temperature, pH) can trigger a switchable phase transition of deep eutectic solvents, and such solvents are known as responsive deep eutectic solvents. In this work, we present the development history, properties, and preparation of responsive deep eutectic solvents, followed by the application of responsive deep eutectic solvents in the extraction and separation of bioactive compounds are presented. Importantly, the mechanism of responsive deep eutectic solvents in the extraction of bioactive compounds is discussed. Finally, the challenges and prospects of responsive deep eutectic solvents in the extraction and separation of bioactive compounds are proposed. Responsive deep eutectic solvents are considered green and efficient solvents. Some methods for extraction and separation of bioactive compounds by responsive deep eutectic solvents can increase the possibility of recycling the deep eutectic solvents, and provide higher efficiency in the extraction and separation field. It is hoped that this will provide a reference for the green and sustainable extraction and separation of various bioactive compounds.

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Ultrasound-assisted deep eutectic solvents extraction of glabridin and isoliquiritigenin from Glycyrrhiza glabra: Optimization, extraction mechanism and in vitro bioactivities

Cited by 44 publications

References 49 publications

Ultrasound-Assisted Natural Deep Eutectic Solvents Extraction of Bilberry Anthocyanins: Optimization, Bioactivities, and Storage Stability

Ultrasound-Assisted Natural Deep Eutectic Solvents Extraction of Bilberry Anthocyanins: Optimization, Bioactivities, and Storage Stability

Conventional vs. Green Extraction Using Natural Deep Eutectic Solvents—Differences in the Composition of Soluble Unbound Phenolic Compounds and Antioxidant Activity

Advances of responsive deep eutectic solvents and application in extraction and separation of bioactive compounds

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