Ultrasonic vocalizations – Novel seizure-related manifestation in rats

Lara-Valderrábano, Leonardo; Ciszek, Robert; Bañuelos-Cabrera, Ivette; Andrade, Pedro; Pitkänen, Asla

doi:10.1016/j.eplepsyres.2022.106927

Cited by 3 publications

(5 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Notably, a recent study showed that rats emit ultrasonic vocalizations during seizures induced by pentylenetetrazole (PTZ). 20 Our results extend on this recent finding by showing that spontaneous seizures are also associated with ultrasonic vocalizations. Collectively, these data suggest that quantitative audio analysis could be developed as a seizure detection tool for Scn1a +/− mice.…”

Section: Discussionsupporting

confidence: 89%

“…Nonetheless, regardless of whether the vocalizations are credited to the seizing mouse or nonseizing cage‐mates, ictal vocalizations are observed in Scn1a +/− mice. Notably, a recent study showed that rats emit ultrasonic vocalizations during seizures induced by pentylenetetrazole (PTZ) 20 . Our results extend on this recent finding by showing that spontaneous seizures are also associated with ultrasonic vocalizations.…”

Section: Discussionsupporting

confidence: 89%

“…Rodent ultrasonic vocalizations have been implicated in parent‐offspring interactions, mating behavior, social interactions, emotional status and to warn of a threat 13–19 . A recent study showed that rats emit ultrasonic vocalizations during induced seizures 20 . Thus, we hypothesized that Scn1a +/− mice emit ultrasonic vocalizations during a seizure.…”

Section: Introductionmentioning

confidence: 95%

“…[13][14][15][16][17][18][19] A recent study showed that rats emit ultrasonic vocalizations during induced seizures. 20 Thus, we hypothesized that Scn1a +/− mice emit ultrasonic vocalizations during a seizure. In the current study, we investigated whether ictal vocalizations either as audible mouse squeaks or ultrasonic vocalizations are characteristics of Scn1a +/− mice.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Ictal vocalizations in the Scn1a^+/− mouse model of Dravet syndrome

et al. 2023

View full text Add to dashboard Cite

ObjectiveIctal vocalizations have shown diagnostic utility in epilepsy patients. Audio recordings of seizures have also been used for seizure detection. The present study aimed to determine whether generalized tonic–clonic seizures in the Scn1a+/− mouse model of Dravet syndrome are associated with either audible mouse squeaks or ultrasonic vocalizations.MethodsAcoustic recordings were captured from group‐housed Scn1a+/− mice undergoing video‐monitoring to quantify spontaneous seizure frequency. We generated audio clips (n = 129) during a generalized tonic–clonic seizure (GTCS) that included 30 seconds immediately prior to the GTCS (preictal) and 30 seconds following the conclusion of the seizure (postictal). Nonseizure clips (n = 129) were also exported from the acoustic recordings. A blinded reviewer manually reviewed the audio clips, and vocalizations were identified as either an audible (<20 kHz) mouse squeak or ultrasonic (>20 kHz).ResultsSpontaneous GTCS in Scn1a+/− mice were associated with a significantly higher number of total vocalizations. The number of audible mouse squeaks was significantly greater with GTCS activity. Nearly all (98%) the seizure clips contained ultrasonic vocalizations, whereas ultrasonic vocalizations were present in only 57% of nonseizure clips. The ultrasonic vocalizations emitted in the seizure clips were at a significantly higher frequency and were nearly twice as long in duration as those emitted in the nonseizure clips. Audible mouse squeaks were primarily emitted during the preictal phase. The greatest number of ultrasonic vocalizations was detected during the ictal phase.SignificanceOur study shows that ictal vocalizations are exhibited by Scn1a+/− mice. Quantitative audio analysis could be developed as a seizure detection tool for the Scn1a+/− mouse model of Dravet syndrome.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ictal vocalizations in the Scn1a^+/− mouse model of Dravet syndrome

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Nevertheless, it is still to be determined whether altered ultrasonic calling behaviour in these experimental models reflects modifications in emotionality, vocal functions, or in both domains. Moreover, a very recent study has shown that the emission of either 22-kHz USVs or 50-kHz USVs may accompany the manifestation of seizures elicited by the administration of pentylenetetrazol in rats, suggesting that USVs could be a novel behavioral marker to be evaluated in rat models of seizures and epileptiform activity (Lara-Valderr abano et al, 2022). Furthermore, the emission of 50-kHz USVs has been extensively investigated in rat paradigms of PD.…”

Section: Rat 50-khz Ultrasonic Vocalizations As a Novel Behavioral Ma...mentioning

confidence: 99%

Mouse and rat ultrasonic vocalizations in neuroscience and neuropharmacology: State of the art and future applications

Premoli

Pietropaolo

Wöhr

et al. 2023

Eur J of Neuroscience

View full text Add to dashboard Cite

Mice and rats emit ultrasonic vocalizations (USVs), which may express their arousal and emotional states, to communicate with each other. There is continued scientific effort to better understand the functions of USVs as a central element of the rodent behavioral repertoire. However, studying USVs is not only important because of their ethological relevance, but also because they are widely applied as a behavioral readout in various fields of biomedical research. In mice and rats, a large number of experimental models of brain disorders exist and studying the emission of USVs in these models can provide valuable information about the health status of the animals and the effectiveness of possible interventions, both environmental and pharmacological. This review (i) provides an updated overview of the contexts in which ultrasonic

show abstract