Ultrasonic enhancement of lipase-catalyzed transesterification for biodiesel production from used cooking oil

Patchimpet, Jaran; Simpson, Benjamin K.; Rui, Xin; Sangkharak, Kanokphorn; Eiad‐ua, Apiluck; Klomklao, Sappasith

doi:10.1007/s13399-021-01790-6

Cited by 7 publications

(4 citation statements)

References 46 publications

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“…At high temperatures, in the instance of 60oC, the magnitude of ultrasonic cavitation impact on the reaction mixture was low, which is similar to the surface tension, bubble formation, and mass transfer, wherein as bubble formation is low the mass transfer is relatively lower. Similarly, other authors have reported similar impacts of temperature on ultrasonic-assisted transesterification reactions (Patchimpet et al 2021).…”

Section: Effect Of Temperaturesupporting

confidence: 62%

Optimization of catalytic properties of Mucor racemosus lipase through immobilization in a biocompatible alginate gelatin hydrogel matrix for free fatty acid production: a sustainable robust biocatalyst for ultrasound-assisted olive oil hydrolysis

et al. 2022

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Immobilization is a key technology that improves the operational stability of enzymes. In this study, alginate-gelatin (Alg-Gel) hydrogel matrix was synthesized and used as immobilization support for Mucor racemosus lipase (Lip). Enzyme catalyzed ultrasound-assisted hydrolysis of olive oil was also investigated. Alg-Gel matrix exhibited high entrapment efficiency (94.5%) with a degradation rate of 42% after 30 days. The hydrolysis of olive oil using Alg-Gel-Lip increased significantly (P < 0.05) as compared to free Lip. Optimum pH and temperature were determined as pH 5.0 and 40 °C, respectively. The Vmax values for free and immobilized Lip were determined to be 5.5 mM and 5.8 mM oleic acid/min/ml, respectively, and the Km values were 2.2 and 2.58 mM/ml respectively. Thermal stability was highly improved for Alg-Gel-Lip (t1/2 650 min and Ed 87.96 kJ/mol) over free Lip (t1/2 150 min and Ed 23.36 kJ/mol). The enzymatic activity of Alg-Gel-Lip was preserved at 96% after four consecutive cycles and 90% of the initial activity after storage for 60 days at 4 °C. Alg-Gel-Lip catalyzed olive oil hydrolysis using ultrasound showed a significant (P < 0.05) increase in hydrolysis rate compared to free Lip (from 0.0 to 58.2%, within the first 2 h). In contrast to traditional methodology, using ultrasonic improved temperature-dependent enzymatic catalyzed reactions and delivered greater reaction yields. Results suggest that Alg-Gel-Lip biocatalyst has great industrial application potential, particularly for free fatty acid production. In addition, the combined use of enzyme and ultrasound has the potential of eco-friendly technology.

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Section: Effect Of Temperaturesupporting

confidence: 62%

Optimization of catalytic properties of Mucor racemosus lipase through immobilization in a biocompatible alginate gelatin hydrogel matrix for free fatty acid production: a sustainable robust biocatalyst for ultrasound-assisted olive oil hydrolysis

et al. 2022

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“…The advantage of using US to increase the productivity of enzymatic transesterifications is well reported, although the current work represents not only the first time that US has been used in an enzymatic transesterification with fermented solids, but also the first time that US has been used in an enzymatic transesterification involving castor oil (Table 3). 15,[38][39][40][41][42][43][44] With other oils, use of US has enabled significant increases in conversions and decreases in reaction time. For example, reaction times have been decreased from 24 h to only 30 min, with conversions near 100%.…”

Section: Discussionmentioning

confidence: 99%

Transesterification of castor oil catalyzed by a fermented solid produced by Burkholderia contaminans using a bioreactor coupled with ultrasound irradiation

Moreira

Alnoch

Luz

et al. 2022

Biotech and App Biochem

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In this work, ultrasound was used to assist the ethanolysis of castor oil in a solvent-free system, catalyzed by a dry fermented solid containing the lipase from Burkholderia contaminans (BCFS). Reactions were done at 45 • C. The maximum conversion in Erlenmeyer flasks was 71% in 96 h, using a loading of 9% (mass of BCFS in relation to the mass of triacylglycerols in the castor oil) and a molar ratio of ethanol:oil of 6:1, with addition of ethanol in 12 steps. In a packed-bed reactor containing 12 g of BCFS, the conversions were 78% in 48 h, and 83% in 72 h with an ethanol to oil molar ratio of 3:1 and treatment with an ultrasound probe, with maximum power of 500 W, frequency of 20 kHz, and 75% of the maximum power. These results are promising given that, with an ultrasound assisted bioreactor, a higher conversion in a shorter time was achieved, with a lower ethanol to oil molar ratio than was the case in the Erlenmeyer flasks without ultrasound.

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“…The advantage of ultrasound assistance to remarkably shorten the reaction time was also reported. The reaction time was reduced from 28 to 3 h with an ultrasonic frequency of 16 kHz and conventional mechanical stirring, a lipase concentration of 30 kUnit, a methanol–oil molar ratio of 4:1 and a reaction temperature of 40°C, as reported by Patchimpet et al 23 . Ultrasonic emulsification was also applied in a packed‐bed reactor to avoid the problem of methanol stratification, and a shorter reaction time and increased yield were observed 107 …”

Section: Process Factors Affecting Lipase‐catalyzed Biodiesel Productionmentioning

confidence: 98%

“…Another advantage of using waste oil as biodiesel feedstock is decreased damage to water and aquatic organisms caused by improper disposal of waste oil 21 . The use of WCO as a feedstock for biodiesel production has attracted much attention 22,23 . Biodiesel synthesized from WCO contains large amounts of saturated and unsaturated fatty acids with high cetane number and flash point indicating good fuel properties.…”

Section: Trends In Feedstocks For Biodiesel Productionmentioning

confidence: 99%

Lipase‐catalyzed production of biodiesel: a critical review on feedstock, enzyme carrier and process factors

Xia,

Lin,

Sayanjali

et al. 2023

Biofuels Bioprod Bioref

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Biodiesel is a green and renewable alternative energy source which can be produced through lipase‐catalyzed transesterification process. Enzymatic biodiesel production has generated much interest owing to its environmentally friendly process, low wastewater treatment requirements and easy product separation. In this work, we attempt to review some of the recent patents and state‐of‐the‐art technology in lipase‐catalyzed transesterification for biodiesel production including feedstock, lipases, novel carriers and process factors. This work will provide useful guidance for researchers to develop cost efficient process for production of biodiesels.

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Ultrasonic enhancement of lipase-catalyzed transesterification for biodiesel production from used cooking oil

Cited by 7 publications

References 46 publications

Optimization of catalytic properties of Mucor racemosus lipase through immobilization in a biocompatible alginate gelatin hydrogel matrix for free fatty acid production: a sustainable robust biocatalyst for ultrasound-assisted olive oil hydrolysis

Optimization of catalytic properties of Mucor racemosus lipase through immobilization in a biocompatible alginate gelatin hydrogel matrix for free fatty acid production: a sustainable robust biocatalyst for ultrasound-assisted olive oil hydrolysis

Transesterification of castor oil catalyzed by a fermented solid produced by Burkholderia contaminans using a bioreactor coupled with ultrasound irradiation

Lipase‐catalyzed production of biodiesel: a critical review on feedstock, enzyme carrier and process factors

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