Ultrasensitive Nano-rt-iPCR for Determination of Polybrominated Diphenyl Ethers in Natural Samples

Abstract

Extensive polybrominated diphenyl ethers (PBDEs) use has resulted in its increasingly widespread presence in the environment. PBDEs release from existing products can still persist and accumulate in the environment as well as in human and wildlife magnifying through the food web. Due to its ultra-trace amount in the environment, a novel ultrasensitive nano-rt-iPCR assay has been developed to determine polybrominated diphenyl ethers in natural samples. Numerous amino-DNA and polyclonal antibody (anti-PBDE) were immobilized onto the single-walled carbon nanotubes (SWCNTs) to form antibody-SWCNTs-DNA signal amplifier used in the proposed immunoassay system. Compared with rt-iPCR, this nano-rt-iPCR assay had a higher ratio of signal DNA, which meant higher signal measured and lower detection limit. This proposed nano-rt-iPCR assay was used to determine PBDEs in water samples ranging from 0.5 pg/L to 0.5ng/L; giving the LOD 1 pg/L. To the best of our knowledge, this nano-rt-iPCR is the most sensitive method for PBDEs detection. Because of that, this method needs no pre-concentration or extractions, using sample sizes as low as 10 µL. In general, this nano-rt-iPCR method will be a useful and potential way for batch detection of ultra-trace PBDEs in the aquatic environment.

SGAEMDA: Predicting miRNA-Disease Associations Based on Stacked Graph Autoencoder

“…Traditional biological wet experiments, such as anchored polymerase chain reaction and reverse transcription polymerase chain reaction, were used in the early years to identify the relationship between miRNAs and diseases, but they all have drawbacks such as complicated experiments, long time periods, and high costs [ 11 , 12 , 13 ]. Several studies in the field of bioinformatics have been developed in recent years, such as drug–drug interactions [ 14 ], drug–target interactions [ 15 ], lncRNA–disease association prediction [ 16 ], and lncRNA–miRNA interaction [ 17 ].…”

A Carboxyl-Functionalized Covalent Organic Polymer for the Efficient Adsorption of Saxitoxin

“…For trace or ultra-trace analysis of organic contaminants from water samples, pretreatment and pre-concentration are often required prior to the quantification [56], and extraction techniques such as solid-phase extraction (SPE) [25], magnetic solid-phase extraction (MSPE) [11,18], and solid-phase microextraction (SPME) [4] have been developed for the concentration of STX. On the other hand, for saxitoxin removal, the most common method is based on adsorption using granular activated carbon (GAC) [34], although other adsorbent materials have also been reported, such as polymeric resins [36], mineral residues [3,12], oyster shell powder [26], and algal polysaccharide gels [29].…”