Ultrasensitive in-vitro monitoring of monoamine neurotransmitters from dopaminergic cells

Abstract

The design of biosensing assay of monoamine neurotransmitters (MANTs) such as epinephrine (Ep), norepinephrine (NE), and dopamine (DA), as well as the monitoring of these MANTs released from dopaminergic cells, are of particular interest. Electrochemical sensors based on the novel construction of nickel oxides (NiO) were fabricated and employed for electrochemical screening of MANTs. A novel NiO-lacy flower-like (NLF) geometrical structure with semi-spherical head surfaces connected with a trunk as an arm was achieved. The designed semi-spherical head associated with abundant and the welldispersed tubular branches with needle-like open ends might lead to the creation of vascular vessels for facile diffusion and suitable accommodation of the released MANTs throughout active and wide-surfacearea coverage, multi-diffusive pores, and caves with connective open macro-/meso-windows along the entire top-view nanoneedles of lacy flower head and trunk. These electrode surfaces possess high-index catalytic site facets associated with the formation of ridges/defects on {110}-top-cover surface dominants for strong binding, fast response, and signaling of MANTs. The NLF-modified electrode enabled high sensitivity for MANTs and a low limit of detection of 6 nM. Ultrasensitive in-vitro monitoring of DA released from dopaminergic cells (such as PC12) was realized. The NLF electrode was used to detect MANTs from its sources (PC12), and it could be used for clinical diagnosis. .

HMT-Controlled Synthesis of Mesoporous NiO Hierarchical Nanostructures and Their Catalytic Role towards the Thermal Decomposition of Ammonium Perchlorate

“…Accordingly, these materials are widely applied in many important fields. Among them, nickel oxide (NiO) is an important semiconductor functional material [2], which is widely used in lithium ion batteries [3], electrocatalysis [4], thermal catalysis [5], gas sensor [6], electrochemistry [7,8], optical sensor [9] and biosensors [10][11][12][13][14] fields. Therefore, the preparation of various mesoporous NiO nanomaterials has become a research hotspot.…”

Rapid and sensitive surface‐enhanced resonance Raman spectroscopy detection for norepinephrine in biofluids

“…In addition, the content of NE is low, which brings great challenge to detect in real sample. Previous studies reported that the pretreatment methods of neurotransmitter detection were applied in biological system . Hence, a pretreatment method must be established for NE detection in biological environment.…”

Facile preparation of surfactant-free Au NPs/RGO/Ni foam for degradation of 4-nitrophenol and detection of hydrogen peroxide

“…In the electrochemical detection method, the pH is one of the factors that control the sensitivity of the electrode [29][30][31][32]. Before detecting the H 2 O 2 , the pH-value of the buffer solution was optimized.…”