Ultrasensitive green spectrofluorimetric approach for quantification of Hg(II) in environmental samples (water and fish samples) using cysteine@MnO₂ dots

Abstract

Mercury (Hg2+) is a natural element present in foods such as fish, water and soil. Exposure to mercury leads to severe toxic effects on the nervous, digestive, and immune systems. Here, a novel, green, and environmentally friendly fluorescent probe decorated with cysteine/MnO2 quantum dots (Cys@MnO2 QDs) was synthesized. This synthesis was carried out using a simple ultrasound technique with the aid of cysteine for fabricating Cys@MnO2 QDs to estimate Hg levels in fish and water samples. In this morphological study, Cys@MnO2 QDs were fully characterized using high‐resolution transmission electron microscopy, zeta potential analysis, fluorescence, ultraviolet–visible and infrared spectroscopy. The fluorescence of the synthesized Cys@MnO2 QDs was significantly quenched by gradually increasing the Hg(II) concentration. The quenching mechanism based on the Hg–S bonds strengthened the utility of the Cys@MnO2 QDs as a novel luminescent nanoprobe. The estimation of Hg was linear in the concentration range 0.7–100.0 ng mL−1 with a limit of quantitation equal to 0.30 ng mL−1. The Cys@MnO2 QDs are fluorescent probes with various benefits such as speed, ease of use, cost‐ effective, and being environmentally friendly; they are easily applied in food manufacturing and for public health improvement.

A new green fluorimetric micelle complexation approach for reduction of the consumed solvent and quantification of avapritinib in biological fluids

“…AVA pills (300.0 mg per tablet) were administered orally to ten healthy human subjects. At 1,2,4,6,8,10,15,20,25,30,35,40, and 50 h, three millilitres of blood were taken intravenously into heparinized tubes. 7 To extract the plasma, blood specimens were centrifuged at 4000 rpm for 30 min.…”

“…The impacts are reliable with already detailed approaches. 8,22,23 The endorsed strategy was effectively utilized to evaluate AVA within the pharmaceutical dose range of Ayvakit® (100 mg per tablet). The rate of recovery ± SD was 102.57 ± 0.45, with t-value and F-value equal to 1.21 and 2.43, respectively, compared with the detailed strategy.…”

“…The rate of recovery ± SD was 102.57 ± 0.45, with t-value and F-value equal to 1.21 and 2.43, respectively, compared with the detailed strategy. 8 To ensure uniformity in dosage units, each prescription pill in a batch must have an active ingredient within the predetermined range of values listed on the label. The AVA method is perfect for checking content consistency because it requires less time and has a greater effect on greenness than traditional analysis techniques.…”

Naphthalimide‐based fluorescent probe for Hg2+ detection and imaging in living cells and zebrafish

“…Low concentrations of heavy metals can be very harmful to human health, including nervous system disorders, organ disease, stunted growth, cancer and even death [1][2][3]. Mercury, as a highly toxic metal, long-term exposure to it can cause seriously adverse effects on the kidney, brain, physiological organs and immune system [4][5][6]. It can interact with several negatively charged groups of enzymes or proteins, leading to cellular dysfunction and affecting the synthesis of protein and nucleic acid and energy production [7,8].…”

Applicability of fluorescamine as a fluorogenic reagent for highly sensitive fluorimetric analysis of the tyrosine kinase inhibitor (avapritinib) in pharmaceuticals and biological samples

“…Furthermore, previously published methodologies [6,7] lacked of bio-analytical validation studies which are now required by US-FDA recommendations. In the presented work, the fluorimetric technique was selected due to its high sensitivity, cost-effectiveness, timesaving characteristics, and its simplicity [8][9][10][11][12][13][14][15]. The presented approach is ultrasensitive (45.00-500.0 ng mL À1 ) and it is more selective for analysis of AVP in human plasma, commercial form, and uniformity test than other published methods [6,7].…”