Ultrasensitive direct impedimetric immunosensor for detection of serum HER2

Sharma, Sucheta; Zapatero-Rodríguez, Julia; Saxena, Rajiv; O’Kennedy, Richard; Srivastava, Sudha

doi:10.1016/j.bios.2018.01.056

Cited by 70 publications

(26 citation statements)

References 19 publications

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“…Though, 60 min. incubation with HER2 was required, the preparation of biosensor receptor layer was less laborious than in the case of other antibody‐based systems as well as a commercially available ELISA test that allowed for HER determination with LOD of 0.123 ng mL −1 .…”

Section: Discussionmentioning

confidence: 99%

“…On the contrary, detection of extracellular domain of HER2 with the application of enzyme‐linked immunosorbent assay (ELISA) is considered to be less invasive and could also indicate the degree of advancement of cancer disease . For that reason HER2 analysis with the use of antibody‐based assays recently became intensively studied and several examples of optical and electrochemical biosensors were reported . The application of the electrochemical techniques for that purpose is particularly promising due to their simplicity, low detection limits and feasibility of miniaturization .…”

Section: Introductionmentioning

confidence: 99%

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Studies on the Affinity‐based Biosensors for Electrochemical Detection of HER2 Cancer Biomarker

et al. 2019

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As cancer diseases are the second main cause of death it is necessary to elaborate fast and efficient early diagnosis methods for their detection. One of the possibilities is the analysis of protein biomarkers, which abnormal concentration in physiological fluids might be an indication of cancer disease progression. Herein, we present the studies on the development of affinity‐based biosensors for electrochemical detection of HER2 protein, which is a common biomarker of breast cancer. The main objective was to verify the possibility of fabrication of HER2‐specific hybrid aptamer‐polyclonal antibody and antibody‐based sandwich sensing layer on gold electrode surface. The effectiveness of each electrode modification step was confirmed using voltammetric and impedimetric techniques in the presence of ferri/ferrocyanide redox couple. It was observed that hybrid construct was unlikely to be formed on the gold electrode due to a higher affinity of secondary polyclonal antibody towards target protein, which resulted in the separation of HER2‐antibody complex from the electrode surface. On the contrary, an antibody‐based sandwich receptor layer allowed for protein discrimination in the range from 1 to 100 ng mL−1 by the application of TMB/H2O2 system and chronoamperometry detection technique. Though, the occurrence of interactions between interfering proteins and antibody‐based layer was noted, it led to at least two times smaller current responses than for HER2 protein.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Studies on the Affinity‐based Biosensors for Electrochemical Detection of HER2 Cancer Biomarker

et al. 2019

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“…For the detection of HER2, a disposable screen printed carbon electrode was modified with gold nanoparticles [15]. The gold nanoparticles allow rapid movement of electrons and provide a biocompatible surface to immobilize small fragments of antibodies in a directed manner, resulting in enhanced binding antigen performance.…”

Section: Human Epidermal Growth Factor Receptor 2 (Her2)mentioning

confidence: 99%

Current and Prospective of Breast Cancer Biomarkers

Benjamin¹,

Lima²

2021

Molecular Biotechnology

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Biomarkers have shown great promise over the past decade the process of drug development more effective and have become an integral part of diagnosis of diseases. Biosensors were integrated with biomarker detection and point-of-care detection for signal amplification, high specificity and sensitivity, rapid response time, low cost, simplicity and multi-analytical testing. In order to detect more sensitively, these particular biomarkers have been explored with the possibility of real-time measurements in order to develop simple and compact systems which can analyze complex specimens. Various biosensors including electrochemical biosensors have recently been developed based on disease-specific biomarkers in the diagnosis of cancer disease. The main objective of the book chapter is to review research with new materials/methods in electrochemical biosensing techniques to detection of breast cancer biomarkers and evaluating latest techniques for detection of important analytes in real samples. In this book chapter, the recent development of electrochemical biosensors of breast cancer biomarkers will be reviewed. Furthermore, recent and future trend application of breast cancer biomarkers will be discussed.

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“…Of these biomarkers, the human epidermal growth factor receptor-2 (HER-2), a speci c oncoprotein, is changed during the development and progression of BC. It was suggested the overexpression of HER-2 in breast cancer cells stimulates proliferation rate and tumor expansion [3,4]. Currently, the diagnosis of HER-2 + BC is based on conventional techniques like cytological analyses using ne-needle aspiration (FNA), uorescence in situ hybridization (FISH), gene expression monitoring, immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA) procedures.…”

Section: Introductionmentioning

confidence: 99%

Precise and Convergence Pattern Was Obtained in the Detection of Mammary Gland Carcinoma Using Molybdenum Enhanced Poly Taurine Nano-biofilms Compared to Pathological Examination

Nasrollahpour

Khalilzadeh

Rahbarghazi‬

et al. 2021

Preprint

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The development of high-performance sensing frameworks for the diagnosis of anaplastic changes is the subject of debate. The lack of on-time diagnosis in patients with suspicious cancers can affect the prognosis and survival rate. As a correlate, the emergence of de novo strategies for the development of transducing frameworks has an inevitable role in advanced biosensing. The combination of green chemistry procedures with eco-friendly and biocompatible materials is of high desirability in this context. The synthesis of new biocompatible and cost-effective nanomaterials to meet the emerging needs of rising demands appeals to new synthetic methodologies. Here, we applied the electrochemical synthesis method to the fabrication of biocompatible and subtly governed Molybdenum trioxide/poly taurine nano-bio films for the monitoring of human epidermal growth factor receptor-2 (HER-2) in sera from breast cancer patients. Morphological and elemental assessments were performed using scanning electron microscope, energy dispersive X-Ray spectroscopy, and dot mapping analyses. In addition, HER-2 immunohistochemistry (IHC) staining was performed on tissue samples and data compared to the values obtained by Molybdenum trioxide/poly taurine nano-bio films. We also noted our platform is eligible for feasible, rapid, and specific determination of HER-2 factor in human samples. The method had a lower limit of quantification of 0.000001 ng mL-1 and a linear dynamic range between 0.1 ng mL-1 and 0.000001 ng mL-1. IHC imaging showed that the degree of anaplastic changes in breast samples (intensity of HER-2 factor) was closely associated with the intensity of signals obtained by our developed immunosensor.

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Ultrasensitive direct impedimetric immunosensor for detection of serum HER2

Cited by 70 publications

References 19 publications

Studies on the Affinity‐based Biosensors for Electrochemical Detection of HER2 Cancer Biomarker

Studies on the Affinity‐based Biosensors for Electrochemical Detection of HER2 Cancer Biomarker

Current and Prospective of Breast Cancer Biomarkers

Precise and Convergence Pattern Was Obtained in the Detection of Mammary Gland Carcinoma Using Molybdenum Enhanced Poly Taurine Nano-biofilms Compared to Pathological Examination

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