Ultrasensitive detection of non-amplified genomic DNA by nanoparticle-enhanced surface plasmon resonance imaging

D’Agata, Roberta; Corradini, Roberto; Ferretti, Cristina; Zanoli, Laura Maria; Gatti, Marcello; Marchelli, Rosangela; Spoto, Giuseppe

doi:10.1016/j.bios.2010.02.008

Cited by 75 publications

(45 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The elimination of PCR and labeling is the focus point in order to develop a new biosensor for early, proper, and low-cost analysis [65]. In this work D'Agata et al describe the results obtained in the ultrasensitive detection of nonamplified genomic DNA.…”

Section: Nanomodified Spr-abbs Applications For In Vitro Diagnosticsmentioning

confidence: 99%

Nanotechnology-Based Surface Plasmon Resonance Affinity Biosensors forIn VitroDiagnostics

Antiochia

Bollella

Favero

et al. 2016

International Journal of Analytical Chemistry

View full text Add to dashboard Cite

In the last decades, in vitro diagnostic devices (IVDDs) became a very important tool in medicine for an early and correct diagnosis, a proper screening of targeted population, and also assessing the efficiency of a specific therapy. In this review, the most recent developments regarding different configurations of surface plasmon resonance affinity biosensors modified by using several nanostructured materials for in vitro diagnostics are critically discussed. Both assembly and performances of the IVDDs tested in biological samples are reported and compared.

show abstract

Section: Nanomodified Spr-abbs Applications For In Vitro Diagnosticsmentioning

confidence: 99%

Nanotechnology-Based Surface Plasmon Resonance Affinity Biosensors forIn VitroDiagnostics

Antiochia

Bollella

Favero

et al. 2016

International Journal of Analytical Chemistry

View full text Add to dashboard Cite

show abstract

“…In fact, the interaction between propagating and localized SPs established when nanoparticles are few nanometers far from the flat metal surface reinforces the local electric field [9–10]. The ultrasensitive detection of nucleic acids has been recently achieved by using streptavidin (SA)-conjugated AuNPs and SPR imaging (SPRI) [11–12]. In this case, the enhanced sensitivity enables the detection of point mutations in non-amplified human genomic DNA with attomolar sensitivity [13], thus offering an excellent cost-effective alternative to time consuming and prone to sample contamination nucleic acid amplification protocols [14].…”

Section: Introductionmentioning

confidence: 99%

Streptavidin-coated gold nanoparticles: critical role of oligonucleotides on stability and fractal aggregation

D’Agata

Palladino

Spoto³

2017

Beilstein J. Nanotechnol.

Self Cite

View full text Add to dashboard Cite

Gold nanoparticles (AuNPs) exhibit unique properties that can be modulated through a tailored surface functionalization, enabling their targeted use in biochemical sensing and medical diagnostics. In particular, streptavidin-modified AuNPs are increasingly used for biosensing purposes. We report here a study of AuNPs surface-functionalized with streptavidin-biotinylated oligonucleotide, focussing on the role played by the oligonucleotide probes in the stabilization/destabilization of the functionalized nanoparticle dispersion. The behaviour of the modified AuNP dispersion as a consequence of the competitive displacement of the biotinylated oligonucleotide has been investigated and the critical role of displaced oligonucletides in triggering the quasi one-dimensional aggregation of nanoparticles is demonstrated for the first time. The thorough understanding of the fundamental properties of bioconjugated AuNPs is of great importance for the design of highly sensitive and reliable functionalized AuNP-based assays.

show abstract

“…In particular SPR imaging (SPRi) [65], the evolution of conventional SPR instrumentation, with the advantage of parallel analysis of many targets, has been applied, or by optic fiber confirming the possibility of detecting directly, by passing Figure 5. The insertion of exogenous genes into the host genome comprises an element called promoter (P) allowing the expression of the coding region (CR) containing the modification i.e.…”

Section: Genetically Modified Organisms (Gmos)mentioning

confidence: 99%

Label free Affinity sensing: application to food analysis

Scarano¹,

Mariani²,

Minunni³

2016

ACTA IMEKO

View full text Add to dashboard Cite

In this paper real-time, label free sensing principle based on Surface Plasmon Resonance (SPR) and gravimetric, i.e. Quartz Crystal Microbalance (QCM) are described and some applications to food analysis problems are reported. Affinity receptors are introduced and examples using antibodies, nucleic acid probes and biomimetic receptors i.e. molecular imprinted polymers (MIP) reported. In particualr pesticide, endotoxins, pathogens, Genetically Modified Organism (GMOs) detection and food origin analysis are reviewed.

show abstract

Ultrasensitive detection of non-amplified genomic DNA by nanoparticle-enhanced surface plasmon resonance imaging

Cited by 75 publications

References 40 publications

Nanotechnology-Based Surface Plasmon Resonance Affinity Biosensors forIn VitroDiagnostics

Nanotechnology-Based Surface Plasmon Resonance Affinity Biosensors forIn VitroDiagnostics

Streptavidin-coated gold nanoparticles: critical role of oligonucleotides on stability and fractal aggregation

Label free Affinity sensing: application to food analysis

Contact Info

Product

Resources

About