Ultrasensitive Detection of Nitrite through Implementation ofN-(1-Naphthyl)ethylenediamine-Grafted Cellulose into a Paper-Based Device

Abstract

Reported herein is the immobilization of N-(1naphthyl)ethylenediamine (NED) on cellulose via an epichlorohydrin (ECH)-based covalent attachment and the implementation of the functionalized cellulose into an ultrasensitive, paper-based device for nitrite detection. The reported functionalization procedure resulted in a 12.9-fold higher functionalization density than the density that results from the previously reported procedures, and the subsequent device allows for nitrite detection limits in synthetic freshwater and real seawater of 0.26 and 0.22 μM, respectively. The sensor is efficient in a wide range of temperature, humidity, turbidity, and salinity conditions and has been successfully applied for nitrite detection in real water samples.

Durable Photoetching Magnetic Biomass‐Based Hydrogel with High Performance

“…As the base‐catalyzed crosslinking agent, ECH is employed to bind alkali lignin and MCC@Fe 3 O 4 chains. [ 15 ] As the second functional crosslinker, Ca 2+ can construct the internal physical network through forming lignin‐metal complexes (lignin‐Ca 2+ ). [ 16 ] As the reaction proceeding, the Ca 2+ can weld the joints served as a bridge between MCC@Fe 3 O 4 and alkali lignin, which can form a network structures with massive robust joints, making the structure more robust and stable.…”

A New Paper-Based Microfluidic Device for Improved Detection of Nitrate in Water

“…Therefore, we decided that immobilizing the detection reagent would capture the color formed and would allow the flow of more sample over the detection zone, which should improve the performance of the device. This was recently successfully achieved by functionalizing one of the two reagents used in the Griess assay on paper, which resulted in an improvement in the detection limit of nitrite [39]. Functionalizing the detection zone is an equipment-free technique to concentrate the analyte of interest in a certain region by flowing a volume of sample that exceeds what is necessary to satisfy the hydrophilic zone [46].…”

“…Sulfanilamide (98%, Alfa Aesar-A1300136), citric acid (≥99%, Alfa Aesar-A103950B), sodium nitrate (≥99.5%, Honeywell Fluka-31440), sodium nitrite (≥99%, Honeywell Fluka-31443), and ASTM Type 1 deionized water (resistivity > 18 MΩ/cm, LabChem-LC267405). A real seawater sample from the Sargasso Sea region known for its low nutrient content [2,39,48] was used to see if the ions usually found in seawater have any effect on the performance of the device. This seawater sample was filtered through a 0.2 µm filter to remove any organic matter prior to its use.…”

“…The first is a new composite material, which we developed in [38], which increases the reduction efficiency of nitrate to nitrite. The second is a functionalized paper, one that has an immobilized Griess reagent [39], which allows the passage of a larger volume of sample over the detection zone. Moreover, device architecture plays a major role in paper-based microfluidics.…”

A water-stable terbium metal–organic framework as a highly sensitive fluorescent sensor for nitrite

“…39 Traditional nitrite ion detection was performed using toxic naphthyl ethylenediamine via colorimetry assay. 40 To avoid the usage of toxic materials, other methods including chromatography, 41 electrochemical techniques 42 and fluorescent technology [43][44][45] have been developed. However, these methods need complicated instruments, tedious detection procedures, and are time-consuming.…”