A New Paper-Based Microfluidic Device for Improved Detection of Nitrate in Water

Charbaji, Amer; Heidari-Bafroui, Hojat; Anagnostopoulos, C.; Faghri, Mohammad

doi:10.3390/s21010102

Cited by 32 publications

(21 citation statements)

References 54 publications

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“…The images were split into red, green, and blue channels: the blue channel intensity was quantified because it showed the greatest change under the scanner conditions (Figure S2 in the SI). It may be attributed that the complementary color of the brownish orange is blue [31,32]. In addition, this can be caused that an orange water-soluble formazan has the maximum absorption between 460 and 480 nm, which corresponds to the blue region of the visible light spectrum [33,34].…”

Section: Cell Viability Of Lncap Cells Cultured On the Papermentioning

confidence: 99%

In Situ Detection of Hydrogen Sulfide in 3D-Cultured, Live Prostate Cancer Cells Using a Paper-Integrated Analytical Device

et al. 2022

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In this study, a paper-integrated analytical device that combined a paper-based colorimetric assay with a paper-based cell culture platform was developed for the in situ detection of hydrogen sulfide (H2S) in three-dimensional (3D)-cultured, live prostate cancer cells. Two kinds of paper substrates were fabricated using a simple wax-printing methodology to form the cell culture and detection zones, respectively. LNCaP cells were seeded directly on the paper substrate and grown in the paper-integrated analytical device. The cell viability and H2S production of LNCaP cells were assessed using a simple water-soluble tetrazolium salt colorimetric assay and H2S-sensing paper, respectively. The H2S-sensing paper showed good sensitivity (sensitivity: 6.12 blue channel intensity/μM H2S, R2 = 0.994) and a limit of quantification of 1.08 μM. As a result, we successfully measured changes in endogenous H2S production in 3D-cultured, live LNCaP cells within the paper-integrated analytical device while varying the duration of incubation and substrate concentration (L-cysteine). This paper-integrated analytical device can provide a simple and effective method to investigate H2S signaling pathways and drug screening in a 3D culture model.

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Section: Cell Viability Of Lncap Cells Cultured On the Papermentioning

confidence: 99%

In Situ Detection of Hydrogen Sulfide in 3D-Cultured, Live Prostate Cancer Cells Using a Paper-Integrated Analytical Device

et al. 2022

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“…Nitrate is part of the nitrogen cycle but contributes to water nutrient pollution when present at elevated concentrations [48] [49]. Jayawardane et al [50] and Charbaji et al [51] used zinc mircoparticles as a reducing agent in paper-based devices meant for the detection of nitrate in water. The zinc microparticles provided the reduction of nitrate to nitrite needed for its detection by the Griess assay.…”

Section: Environmental Applicationsmentioning

confidence: 99%

Literature Review of the Use of Zinc and Zinc Compounds in Paper-Based Microfluidic Devices

Charbaji¹,

Heidari-Bafroui²,

Anagnostopoulos³

et al. 2021

JMMCE

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Zinc and its compounds, alloys and composites play an important role in the modern day world and find application in almost every aspect that can improve the quality of our lives. This ranges from supplements and pharmaceuticals that are meant to improve our health and wellbeing to additives meant to guard or reduce corrosion in metals. However, over the past several years, a new area of technology has been garnering a great deal of attention and has made use of zinc and its compounds. This is with reference to paper-based microfluidic technology that offers several advantages and that keeps expanding in the amount of applications it covers. In this paper, a review is offered for the applications that have used zinc or zinc compounds in paper-based microfluidic devices.

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“…One recent method for conducting inexpensive analysis of nutrients in water, which is field deployable, disposable, and easy-to-use by an unskilled operator, utilizes paper-based devices and the colorimetric method [ 28 ]. Thus far, only three colorimetric paper-based assays have been proposed for the detection of phosphate in water.…”

Section: Introductionmentioning

confidence: 99%

A Colorimetric Dip Strip Assay for Detection of Low Concentrations of Phosphate in Seawater

Heidari-Bafroui

Charbaji

Anagnostopoulos

et al. 2021

Sensors

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Nutrient pollution remains one of the greatest threats to water quality and imposes numerous public health and ecological concerns. Phosphate, the most common form of phosphorus, is one of the key nutrients necessary for plant growth. However, phosphate concentration in water should be carefully monitored for environmental protection requirements. Hence, an easy-to-use, field-deployable, and reliable device is needed to measure phosphate concentrations in the field. In this study, an inexpensive dip strip is developed for the detection of low concentrations of phosphate in water and seawater. In this device, ascorbic acid/antimony reagent was dried on blotting paper, which served as the detection zone, and was followed by a wet chemistry protocol using the molybdenum method. Ammonium molybdate and sulfuric acid were separately stored in liquid form to significantly improve the lifetime of the device and enhance the reproducibility of its performance. The device was tested with deionized water and Sargasso Sea seawater. The limits of detection and quantification for the optimized device using a desktop scanner were 0.134 ppm and 0.472 ppm for phosphate in water and 0.438 ppm and 1.961 ppm in seawater, respectively. The use of the portable infrared lightbox previously developed at our lab improved the limits of detection and quantification by a factor of three and were 0.156 ppm and 0.769 ppm for the Sargasso Sea seawater. The device’s shelf life, storage conditions, and limit of detection are superior to what was previously reported for the paper-based phosphate detection devices.

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A New Paper-Based Microfluidic Device for Improved Detection of Nitrate in Water

Cited by 32 publications

References 54 publications

In Situ Detection of Hydrogen Sulfide in 3D-Cultured, Live Prostate Cancer Cells Using a Paper-Integrated Analytical Device

In Situ Detection of Hydrogen Sulfide in 3D-Cultured, Live Prostate Cancer Cells Using a Paper-Integrated Analytical Device

Literature Review of the Use of Zinc and Zinc Compounds in Paper-Based Microfluidic Devices

A Colorimetric Dip Strip Assay for Detection of Low Concentrations of Phosphate in Seawater

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