Ultrasensitive detection of lysozyme in droplet-based microfluidic devices

Giuffrida, Maria Chiara; Cigliana, Giovanni; Spoto, Giuseppe

doi:10.1016/j.bios.2017.12.042

Cited by 34 publications

(28 citation statements)

References 44 publications

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“…Luminescence, as a general term is related to the energy transition between molecular orbitals that produces an emission of light. When the excitation of the molecules is caused by a chemical reaction, this light emission is chemiluminescence [ 27 , 33 , 36 , 58 , 71 , 81 ]. The emission that accompanies an electrochemical reaction is known as electrochemiluminescence [ 79 ].…”

Section: Detection Methods and Assay Formatsmentioning

confidence: 99%

“…In recent years, microfluidic chips have aroused increasing interest for various application because of their desirable features, such as the smaller sample amount needed and lowered reagent consumption. The substrate materials of microfluidic chips such as polymers (e.g., PDMS, PMMA, PS) [ 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ], ceramics (e.g., glass) [ 6 , 13 , 14 , 16 , 17 , 18 , 19 , 21 , 25 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 ], and semiconduct...…”

Section: Classification Of Microdevicesmentioning

confidence: 99%

“…Competitive binding of the aptamer and the target decrease the affinity of the adsorption by GO, this condition may release the aptamer from the GO surface, thus resulting in the fluorescence recovery (“turn-on” of fluorescence intensity). Giuffrida et al [ 33 ] also used microfluidic droplets with a T-junction channel to detect lysozyme. However, their device had six inlets and was equipped with a mixing region and a chaotic mixer channel to allow chemiluminescence detection.…”

Section: Classification Of Microdevicesmentioning

confidence: 99%

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Recent Microdevice-Based Aptamer Sensors

et al. 2018

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Since the systematic evolution of ligands by exponential enrichment (SELEX) method was developed, aptamers have made significant contributions as bio-recognition sensors. Microdevice systems allow for low reagent consumption, high-throughput of samples, and disposability. Due to these advantages, there has been an increasing demand to develop microfluidic-based aptasensors for analytical technique applications. This review introduces the principal concepts of aptasensors and then presents some advanced applications of microdevice-based aptasensors on several platforms. Highly sensitive detection techniques, such as electrochemical and optical detection, have been integrated into lab-on-a-chip devices and researchers have moved towards the goal of establishing point-of-care diagnoses for target analyses.

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Section: Detection Methods and Assay Formatsmentioning

confidence: 99%

Section: Classification Of Microdevicesmentioning

confidence: 99%

Section: Classification Of Microdevicesmentioning

confidence: 99%

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Recent Microdevice-Based Aptamer Sensors

et al. 2018

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“…Finally, H 2 O 2 was injected into the transportation unit to re-suspend the sandwich-like complex magnetic beads (e) which was then followed by the transporting of target-aptamer-bead complex with acridinium to the NaOH chamber where CL signals were monitored with a luminometer (f). Giuffrida et al have reported a chemiluminescent sensor that employs digital microfluidics for aptamer-based detection of human lysozyme [100]. With this droplet microfluidics-based technique that uses gold nanoparticles as an enhancement agent for the CL signal, a detection limit of 44.6 fM was achieved using a sample volume as low as 1 µL and a detection time in the range of 10 minutes.…”

Section: Chemiluminescence and Electrochemiluminescence-based Detectionmentioning

confidence: 99%

Lab-on-a-Chip Systems for Aptamer-Based Biosensing

Khan

Song

2020

Micromachines

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Aptamers are oligonucleotides or peptides that are selected from a pool of random sequences that exhibit high affinity toward a specific biomolecular species of interest. Therefore, they are ideal for use as recognition elements and ligands for binding to the target. In recent years, aptamers have gained a great deal of attention in the field of biosensing as the next-generation target receptors that could potentially replace the functions of antibodies. Consequently, it is increasingly becoming popular to integrate aptamers into a variety of sensing platforms to enhance specificity and selectivity in analyte detection. Simultaneously, as the fields of lab-on-a-chip (LOC) technology, point-of-care (POC) diagnostics, and personal medicine become topics of great interest, integration of such aptamer-based sensors with LOC devices are showing promising results as evidenced by the recent growth of literature in this area. The focus of this review article is to highlight the recent progress in aptamer-based biosensor development with emphasis on the integration between aptamers and the various forms of LOC devices including microfluidic chips and paper-based microfluidics. As aptamers are extremely versatile in terms of their utilization in different detection principles, a broad range of techniques are covered including electrochemical, optical, colorimetric, and gravimetric sensing as well as surface acoustics waves and transistor-based detection.

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“…Among the approaches tested, different nanoparticles, such as quantum dots, gold and silver NP's, were modified with specific antibodies against different biomarkers known to be associated with cancer ( Figure 4). As shown in Table 1, [50][51][52][53][54][55][56][57][58][59][60][61][62][63][64][65] several studies have demonstrated different efficiencies regarding detection ranges of single or multiple biomarker detection.…”

Section: Protein Detection and Quantificationmentioning

confidence: 99%

Finding the perfect match between nanoparticles and microfluidics to respond to cancer challenges

Maia

Reis

Oliveira

2020

Nanomedicine: Nanotechnology, Biology and Medicine

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The clinical translation of new cancer theranostic has been delayed by inherent cancer's heterogeneity. Additionally, this delay has been enhanced by the lack of an appropriate in vitro model, capable to produce accurate data. Nanoparticles and microfluidic devices have been used to obtain new and more efficient strategies to tackle cancer challenges. On one hand, nanoparticles-based therapeutics can be modified to target specific cells, and/or molecules, and/or modified with drugs, releasing them over time. On the other hand, microfluidic devices allow the exhibition of physiologically complex systems, incorporation of controlled flow, and control of the chemical environment. Herein, we review the use of nanoparticles and microfluidic devices to address different cancer challenges, such as detection of CTCs and biomarkers, point-of-care devices for early diagnosis and improvement of therapies. The future perspectives of cancer challenges are also addressed herein.

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Ultrasensitive detection of lysozyme in droplet-based microfluidic devices

Cited by 34 publications

References 44 publications

Recent Microdevice-Based Aptamer Sensors

Recent Microdevice-Based Aptamer Sensors

Lab-on-a-Chip Systems for Aptamer-Based Biosensing

Finding the perfect match between nanoparticles and microfluidics to respond to cancer challenges

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