Recent Microdevice-Based Aptamer Sensors

Abstract

Since the systematic evolution of ligands by exponential enrichment (SELEX) method was developed, aptamers have made significant contributions as bio-recognition sensors. Microdevice systems allow for low reagent consumption, high-throughput of samples, and disposability. Due to these advantages, there has been an increasing demand to develop microfluidic-based aptasensors for analytical technique applications. This review introduces the principal concepts of aptasensors and then presents some advanced applications of microdevice-based aptasensors on several platforms. Highly sensitive detection techniques, such as electrochemical and optical detection, have been integrated into lab-on-a-chip devices and researchers have moved towards the goal of establishing point-of-care diagnoses for target analyses.

Detection of the SARS-CoV-2 spike protein in saliva with Shrinky-Dink© electrodes

“…Aptamers that selectively bind to specific targets have extensively been demonstrated to be promising as a diagnostic tool for disease detection through molecular recognition, and have been prominently investigated. 21,45,[49][50][51][52][53][54][55] In this study, an aptamer specific to the RBD region of the SARS-CoV-2 spike protein was selected as the probe for the detection of the SARS-CoV-2 in saliva.…”

Aptamers for Viral Detection and Inhibition

“… 119 , 120 Chemiluminescent methods have often been implemented as signal amplifiers to provide detection limits of 10 –12 to 10 –21 mol. 24 , 121 , 122 Ahn et al developed an aptasensor to detect the nucleocapsid protein of SARS coronavirus (SARS-CoV). 123 This aptasensor utilizes an aptamer–target–antibody sandwich method, where the nucleocapsid proteins are recognized by surface-immobilized aptamers.…”

Anti‐VEGF‐R2 Aptamer and RGD Peptide Synergize in a Bifunctional Hydrogel for Enhanced Angiogenic Potential

“…[ 14 ] Aptamers have been used in a myriad of applications from treating ocular vascular disease to use as sensors. [ 15–17 ] In hydrogel applications, DNA aptamers have been used to either sequester or release species such as growth factors. [ 18–23 ] Chitosan films with immobilized antifibronectin aptamers on the surface were shown to help improve cell attachment and function.…”