Ultrasensitive Detection of DNA by PNA and Nanoparticle‐Enhanced Surface Plasmon Resonance Imaging

Chemical Modification of Oligonucleotides for Therapeutic, Bioanalytical and other Applications

“…The ratio of the response to matched and mismatched sequences was 7.4 at 1-500 fm concentration, which is suitable for detecting DNA samples without amplification. [93] The use of modified oligonucleotides for the detection of DNA/RNA was further demonstrated by the work of Appella and co-workers who used PNA probes in a sandwich hybridisation assay to detect anthrax DNA (Figure 4). A PNA capture probe targeted to the protective antigen portion of the anthrax genome was immobilised to a DNA-Bind plate.…”

Ultrasensitive detection of non-amplified genomic DNA by nanoparticle-enhanced surface plasmon resonance imaging

“…The advantages offered by surface plasmon resonance imaging (SPRI) for the label-free, ultrasensitive and multiplexed detection of DNA and RNA have been recently demonstrated (Wark et al, 2008;Ruemmele et al, 2008;D'Agata et al, 2008). The detection of 7 fM genomic DNA has been obtained by using an enzymatically amplified SPRI-based analytical approach (Goodrich et al, 2004) while the direct detection of 10 ppm solutions of plant, bovine and human genomic DNAs has been obtained by using spectral SPR (Minunni et al, 2007).…”

A simple hairpin DNA sensor for label-free detection of sub-attomole DNA target

“…Recently, in order to boost up the detection sensitivity and satisfy the aim of trace analysis, some improvements have been adopted. On the one hand, various amplification schemes have been used in conjunction with SPR for DNA sample analyses [10,11]; on the other hand, more and more cheaply and ultrasensitively homebuilt SPR instruments have appeared [3,6,12]. Combined with flow injection, the home-built high-resolution SPR (FI-SPR) was used for DNA sensitive detection [3,6] and with signal amplification effect of gold nanoparticles (Au-NPs) in a sandwich DNA hybridization assay, a detection limit of 1.38 fM was obtained [3].…”