Ultrasensitive detection of avian influenza A (H7N9) virus using surface-enhanced Raman scattering-based lateral flow immunoassay strips

Xiao, Meng; Xie, Kai‐Xin; Dong, Xiaohui; Wang, Lei; Huang, Chao; Xu, Fei; Xiao, Wei; Jin, Meilin; Huang, Boyan; Tang, Yong

doi:10.1016/j.aca.2018.11.056

Cited by 82 publications

(45 citation statements)

References 48 publications

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“…As for non-aptamer SERS-based techniques, detection of influenza genome via complementary DNA probe was shown to have no advantages in terms of LOD having sensitivity 2.7 attomole of RNA per probe (nearly 1.5·10 5 virus particles per sample) [52]. SERS-based lateral flow immunoassay had 10-fold higher LOD as tested for H7N9 influenza virus [53].…”

Section: Resultsmentioning

confidence: 99%

Highly sensitive detection of influenza virus with SERS aptasensor

Кукушкин¹,

Ivanov²,

Novoseltseva³

et al. 2019

PLoS ONE

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Highly sensitive and rapid technology of surface enhanced Raman scattering (SERS) was applied to create aptasensors for influenza virus detection. SERS achieves 10 6 −10 9 times signal amplification, yielding excellent sensitivity, whereas aptamers to hemagglutinin provide a specific recognition of the influenza virus. Aptamer RHA0385 was demonstrated to have essentially broad strain-specificity toward both recombinant hemagglutinins and the whole viruses. To achieve high sensitivity, a sandwich of primary aptamers, influenza virus and secondary aptamers was assembled. Primary aptamers were attached to metal particles of a SERS substrate, and influenza viruses were captured and bound with secondary aptamers labelled with Raman-active molecules. The signal was affected by the concentration of both primary and secondary aptamers. The limit of detection was as low as 1 · 10 −4 hemagglutination units per probe as tested for the H3N2 virus (A/England/42/72). Aptamer-based sensors provided recognition of various influenza viral strains, including H1, H3, and H5 hemagglutinin subtypes. Therefore, the aptasensors could be applied for fast and low-cost strain-independent determination of influenza viruses.

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Section: Resultsmentioning

confidence: 99%

Highly sensitive detection of influenza virus with SERS aptasensor

Кукушкин¹,

Ivanov²,

Novoseltseva³

et al. 2019

PLoS ONE

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“…Similarly, diverse pathogens can also be detected with this approach, as reported for such as Escherichia coli, Staphylococcus aureus, and different mycotoxins in real samples using MF-SERS [66], [67]. Eventually, the detection of different viruses as human immunodeficiency virus, HIV, type 1 [68] and avian influenza virus, AIV, H7N9 [69] has been possible with the development of microfluidic devices and their link-up with SERS due to their ability to discriminate selectively the infected cells. All these proves of concept of SERS in medical applications and the miniaturization of the instrumentation permitted by the microfluidic devices have led to the fabrication of point-of-care (POC) devices.…”

Section: Mf-sers: a Full Range Of Applicationsmentioning

confidence: 85%

Microfluidics and Surface-Enhanced Raman Spectroscopy: A Perfect Match for New Analytical Tools

Ochoa-Vazquez¹,

Kharisov²,

Morquecho³

et al. 2019

IEEE Trans.on Nanobioscience

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In this perspective article, we emphasize the combination of Surface-Enhanced Raman Spectroscopy (SERS) and Microfluidic devices. SERS approaches have been widely studied and used for multiple applications including trace molecules detection, in situ analysis of biological samples and monitoring or, all of them with good results, however still with limitations of the technique, for example regarding with improved precision and reproducibility. These implications can be overcome by microfluidic approaches. The resulting coupling Microfluidics-SERS (MF-SERS) has recently gained increasing attention by creating thundering opportunities for the analytical field. For this purpose, we introduce some of the strategies developed to implement SERS within microfluidic reactor along with a brief overview of the most recent MF-SERS applications for biology, health and environmental concerns. Eventually, we will discuss future research opportunities of such systems.

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“…To our knowledge we have described the first Nbs capable of distinguishing (HP) A/Guangdong/17SF003/2016 (H7N9) strain from (LP) A/Hong Kong/125/2017 (H7N9) and have been able to correlate this specificity to natural sequence divergence at K164 in the HA1 head domain. Since H7N9 presents in poultry with only mild symptoms, early warning signs of a pandemic may be extremely difficult to detect and the availability of specific Nbs formatted for rapid detection of this antigenic difference may be useful for influenza surveillance in the field using low cost biosensors 54 .…”

Section: Discussionmentioning

confidence: 99%

Nanobodies mapped to cross-reactive and divergent epitopes on A(H7N9) influenza hemagglutinin using yeast display

Gaiotto

Ramage

Ball

et al. 2021

Sci Rep

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Influenza H7N9 virus continues to cause infections in humans and represents a significant pandemic risk. During the most recent 5th epidemic wave in 2016/17 two distinct lineages with increased human infections and wider geographical spread emerged. In preparation for any future adaptations, broadly reactive antibodies against H7N9 are required for surveillance, therapy and prophylaxis. In this study we have isolated a panel of nanobodies (Nbs) with broad reactivity across H7 influenza strains, including H7N9 strains between 2013 and 2017. We also describe Nbs capable of distinguishing between the most recent high and low pathogenicity Yangtze River Delta lineage H7N9 strains. Nanobodies were classified into 5 distinct groups based on their epitope footprint determined using yeast display and mutational scanning. The epitope footprint of Nbs capable of distinguishing high pathogenic (HP) A/Guangdong/17SF003/2016 from low pathogenic (LP) A/Hong Kong/125/2017 (H7N9) were correlated to natural sequence divergence in the head domain at lysine 164. Several Nbs binding to the head domain were capable of viral neutralisation. The potency of one nanobody NB7-14 could be increased over 1000-fold to 113 pM by linking two Nbs together. Nbs specific for distinct epitopes on H7N9 may be useful for surveillance or therapy in human or veterinary settings.

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Ultrasensitive detection of avian influenza A (H7N9) virus using surface-enhanced Raman scattering-based lateral flow immunoassay strips

Cited by 82 publications

References 48 publications

Highly sensitive detection of influenza virus with SERS aptasensor

Highly sensitive detection of influenza virus with SERS aptasensor

Microfluidics and Surface-Enhanced Raman Spectroscopy: A Perfect Match for New Analytical Tools

Nanobodies mapped to cross-reactive and divergent epitopes on A(H7N9) influenza hemagglutinin using yeast display

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