Highly sensitive detection of influenza virus with SERS aptasensor

Abstract

Highly sensitive and rapid technology of surface enhanced Raman scattering (SERS) was applied to create aptasensors for influenza virus detection. SERS achieves 10 6 −10 9 times signal amplification, yielding excellent sensitivity, whereas aptamers to hemagglutinin provide a specific recognition of the influenza virus. Aptamer RHA0385 was demonstrated to have essentially broad strain-specificity toward both recombinant hemagglutinins and the whole viruses. To achieve high sensitivity, a sandwich of primary aptamers, influenza virus and secondary aptamers was assembled. Primary aptamers were attached to metal particles of a SERS substrate, and influenza viruses were captured and bound with secondary aptamers labelled with Raman-active molecules. The signal was affected by the concentration of both primary and secondary aptamers. The limit of detection was as low as 1 · 10 −4 hemagglutination units per probe as tested for the H3N2 virus (A/England/42/72). Aptamer-based sensors provided recognition of various influenza viral strains, including H1, H3, and H5 hemagglutinin subtypes. Therefore, the aptasensors could be applied for fast and low-cost strain-independent determination of influenza viruses.

Aptamers used for biosensors and targeted therapy

“…Until now, many papers concerning the preparation of aptamer-based biosensors have been published. These aptasensors were constructed using various methodologies, such as optical aptasensors (fluorescence-based optical aptasensors and colorimetric-based optical aptasensors), electrochemical aptasensors, chemiluminescent aptasensors, and surface-enhanced Raman scattering (SERS) aptasensors [ [95] , [96] , [97] , [98] , [99] ]. However, most of the principles of these aptasensors have some versatile or analogous elements.…”

Recent Development of Aptasensor for Influenza Virus Detection

“…Kukushkin's group conducted a sandwich SERS assay using two different aptamers with high sensitivity for HA 81 . After fixing the thiol-attached primary aptamer to Ag particles, the virus was bound to generate a Raman signal through a secondary aptamer bound to Cy3, resulting in 10 6 -10 9 -fold signal amplification.…”

An Analysis Review, Detection Coronavirus Disease 2019 (COVID-19) based on Biosensor Application

“…Surface-enhanced Raman Scatter (SERS) technology was applied to flu virusdetection. SERS has 10 6 − 10 9 times signal amplification, which provides excellent sensitivity have precise influenza virus identification [143]. Reliable viral detection, sizing, and filtering is essential for biosensors, environmental monitoring, and quality control.…”