Ultrasensitive detection of anti-p53 autoantibodies based on nanomagnetic capture and separation with fluorescent sensing nanobioprobe for signal amplification

Adeniyi, Omotayo; Ngqinambi, A; Mashazi, Philani

doi:10.1016/j.bios.2020.112640

Cited by 18 publications

(5 citation statements)

References 29 publications

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“…Moreover, the results from this proposed method could be directly read without transducing by high-precision signal acquisition equipment or devices, in contrast to other sophisticated biosensing methods 40 – 43 .…”

Section: Resultsmentioning

confidence: 99%

Acoustofluidics-enhanced biosensing with simultaneously high sensitivity and speed

Li,

Zhao,

Yang

et al. 2024

Microsyst Nanoeng

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Simultaneously achieving high sensitivity and detection speed with traditional solid-state biosensors is usually limited since the target molecules must passively diffuse to the sensor surface before they can be detected. Microfluidic techniques have been applied to shorten the diffusion time by continuously moving molecules through the biosensing regions. However, the binding efficiencies of the biomolecules are still limited by the inherent laminar flow inside microscale channels. In this study, focused traveling surface acoustic waves were directed into an acoustic microfluidic chip, which could continuously enrich the target molecules into a constriction zone for immediate detection of the immune reactions, thus significantly improving the detection sensitivity and speed. To demonstrate the enhancement of biosensing, we first developed an acoustic microfluidic chip integrated with a focused interdigital transducer; this transducer had the ability to capture more than 91% of passed microbeads. Subsequently, polystyrene microbeads were pre-captured with human IgG molecules at different concentrations and loaded for detection on the chip. As representative results, ~0.63, 2.62, 11.78, and 19.75 seconds were needed to accumulate significant numbers of microbeads pre-captured with human IgG molecules at concentrations of 100, 10, 1, and 0.1 ng/mL (~0.7 pM), respectively; this process was faster than the other methods at the hour level and more sensitive than the other methods at the nanomolar level. Our results indicated that the proposed method could significantly improve both the sensitivity and speed, revealing the importance of selective enrichment strategies for rapid biosensing of rare molecules.

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Section: Resultsmentioning

confidence: 99%

Acoustofluidics-enhanced biosensing with simultaneously high sensitivity and speed

Li,

Zhao,

Yang

et al. 2024

Microsyst Nanoeng

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“…The choice of NaOH is due to the dissolution of the silica matrix being accelerated at a higher pH and enhanced in the presence of cations such as sodium and potassium ions. 39…”

Section: Resultsmentioning

confidence: 99%

Antibody fluorescein-doped silica nanobioconjugates for the ultrasensitive detection of prostate-specific antigen

Msutu,

Adeniyi,

Mashazi

2024

Sens. Diagn.

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“…In addition to the aforementioned AD immunosensors, sensors based on similar technological approaches that can detect cancer-related autoantibodies, such as anti-p53 antibodies, have been developed [ 73 ]. Comprehensive reviews focusing on biosensors/immunosensors for AD- and cancer-related autoantibodies have been recently published [ 1 , 15 ].…”

Section: Ad Immunosensors Detecting Autoantibodiesmentioning

confidence: 99%

Immunosensors for Autoimmune-Disease-Related Biomarkers: A Literature Review

Karachaliou

Livaniou

2023

Sensors

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Immunosensors are a special class of biosensors that employ specific antibodies for biorecognition of the target analyte. Immunosensors that target disease biomarkers may be exploited as tools for disease diagnosis and/or follow-up, offering several advantages over conventional analytical techniques, such as rapid and easy analysis of patients’ samples at the point-of-care. Autoimmune diseases have been increasingly prevalent worldwide in recent years, while the COVID-19 pandemic has also been associated with autoimmunity. Consequently, demand for tools enabling the early and reliable diagnosis of autoimmune diseases is expected to increase in the near future. To this end, interest in immunosensors targeting autoimmune disease biomarkers, mainly, various autoantibodies and specific pro-inflammatory proteins (e.g., specific cytokines), has been rekindled. This review article presents most of the immunosensors proposed to date as potential tools for the diagnosis of various autoimmune diseases, such as type 1 diabetes, rheumatoid arthritis, and multiple sclerosis. The signal transduction and the immunoassay principles of each immunosensor have been suitably classified and are briefly presented along with certain sensor elements, e.g., special nano-sized materials used in the construction of the immunosensing surface. The main concluding remarks are presented and future perspectives of the field are also briefly discussed.

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Ultrasensitive detection of anti-p53 autoantibodies based on nanomagnetic capture and separation with fluorescent sensing nanobioprobe for signal amplification

Cited by 18 publications

References 29 publications

Acoustofluidics-enhanced biosensing with simultaneously high sensitivity and speed

Acoustofluidics-enhanced biosensing with simultaneously high sensitivity and speed

Antibody fluorescein-doped silica nanobioconjugates for the ultrasensitive detection of prostate-specific antigen

Immunosensors for Autoimmune-Disease-Related Biomarkers: A Literature Review

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