Ultrasensitive chemiluminescence immunoassay with enhanced precision for the detection of cTnI amplified by acridinium ester-loaded microspheres and internally calibrated by magnetic fluorescent nanoparticles

Zhao, Huan; Lin, Qifeng; Huang, Li; Zhai, Yunfeng; Liu, Yuan; Deng, Yan; Su, Enben; He, Nongyue

doi:10.1039/d0nr08008j

Cited by 85 publications

(32 citation statements)

References 39 publications

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“…The LOD of 0.116 pg/mL and an LDR of 0.1-40 ng/mL, which are relevant for the clinical settings. A more recent example of fluorescent-loaded anti-cTnI was reported by He and coworkers where acridinium ester-loaded into poly[(N-isopropyl acryl-amide)-co-(methacrylic acid)] (P(NIPAM-co-MAA)) microspheres were conjugated to detection antibody [44]. The capture antibody is immobilized on magnetic fluorescent nanobeads.…”

Section: Scheme 2 Hydrolysis Of Coumarinmentioning

confidence: 99%

Fluorescent Immunoassays for Detection and Quantification of Cardiac Troponin I: A Short Review

2021

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Cardiovascular diseases are considered one of the major causes of human death globally. Myocardial infarction (MI), characterized by a diminished flow of blood to the heart, presents the highest rate of morbidity and mortality among all other cardiovascular diseases. These fatal effects have triggered the need for early diagnosis of appropriate biomarkers so that countermeasures can be taken. Cardiac troponin, the central key element of muscle regulation and contraction, is the most specific biomarker for cardiac injury and is considered the “gold standard”. Due to its high specificity, the measurement of cardiac troponin levels has become the predominant indicator of MI. Various forms of diagnostic methods have been developed so far, including chemiluminescence, fluorescence immunoassay, enzyme-linked immunosorbent assay, surface plasmon resonance, electrical detection, and colorimetric protein assays. However, fluorescence-based immunoassays are considered fast, accurate and most sensitive of all in the determination of cardiac troponins post-MI. This review represents the strategies, methods and levels of detection involved in the reported fluorescence-based immunoassays for the detection of cardiac troponin I.

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Section: Scheme 2 Hydrolysis Of Coumarinmentioning

confidence: 99%

Fluorescent Immunoassays for Detection and Quantification of Cardiac Troponin I: A Short Review

2021

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“…Researchers have made great efforts to develop a range of drug delivery systems [97][98][99]. It is well known that magnetic particles have many biomedical applications [100][101][102][103][104], such as detection [105,106], drug delivery [107], etc. Magnetic materials, especially Fe 3 O 4 , have been considered as effective drug delivery systems due to their hollow structures.…”

Section: Drug Delivery Systemmentioning

confidence: 99%

Applications of Aptamer-Bound Nanomaterials in Cancer Therapy

et al. 2021

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Cancer is still a major disease that threatens human life. Although traditional cancer treatment methods are widely used, they still have many disadvantages. Aptamers, owing to their small size, low toxicity, good specificity, and excellent biocompatibility, have been widely applied in biomedical areas. Therefore, the combination of nanomaterials with aptamers offers a new method for cancer treatment. First, we briefly introduce the situation of cancer treatment and aptamers. Then, we discuss the application of aptamers in breast cancer treatment, lung cancer treatment, and other cancer treatment methods. Finally, perspectives on challenges and future applications of aptamers in cancer therapy are discussed.

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“…It is worth noting that magnetic nanomaterials (MNMs) deploy extensive applications in the fields of bioassay, biomedicine, catalysis, and energy storage [ 10 – 13 ]. Benefiting from their tunable chemical composition, intrinsic magnetism, and large specific surface area, MNMs can be considered as nanoplatforms in CL bioassays [ 14 – 16 ]. To date, various magnetic materials as carriers have been used for the development of CF-NMs, such as magnetic mesoporous carbon materials [ 17 ], Fe 3 O 4 magnetic beads [ 18 ], magnetic graphene materials [ 19 ], and magnetic hydrogels [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Chemiluminescent magnetic nanoparticles with good catalytic activity and rapid separation capability and sensitive sensing for H2O2

Wang

Cui

2021

Anal Bioanal Chem

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Graphical abstract It is of considerable importance to develop chemiluminescent functionalized nanomaterials (CF-NMs) with good catalytic activity, high chemiluminescence (CL) efficiency and good stability, and rapid magnetic separation capability, achieving excellent performance in CL biosensing. In this study, N-(4-aminobutyl)-N-ethylisoluminol (ABEI)-functionalized CuFe 2 O 4 magnetic nanomaterial (ABEI/CuFe 2 O 4 ) with high catalytic activity was synthesized by virtue of a solvothermal and post-functionalization method. ABEI/CuFe 2 O 4 showed outstanding CL properties, superior to ABEI-CuFe 2 O 4 in liquid phase. This reveals that the immobilization of ABEI on the surface of CuFe 2 O 4 exhibits unique heterogeneous catalytic property. The catalytic ability of CuFe 2 O 4 was better than that of CoFe 2 O 4 , ZnFe 2 O 4 , MnFe 2 O 4 , and NiFe 2 O 4 . It is suggested that the peroxide-like activity as well as Cu 2+ and Cu 0 enriched on the surface of ABEI/CuFe 2 O 4 opened up a dual route for synergistic catalysis of H 2 O 2 . ABEI/CuFe 2 O 4 also demonstrated good superparamagnetism and magnetic separation could be carried out in 2 min, which is advantageous for the separation and purification of ABEI/CuFe 2 O 4 during the synthetic procedures and bioassays. Owing to the sensitive response of ABEI/CuFe 2 O 4 to H 2 O 2 , an enzyme-free sensor was developed for the detection of H 2 O 2 with a wide linear range over 5 orders of magnitude of H 2 O 2 concentrations and a low detection limit of 5.6 nM. The as-developed sensor is sensitive, stable, and convenient. This work provides a new family member of nanomaterials with good magnetism and CL activity as well as good stability. The developed ABEI/CuFe 2 O 4 shows great prospects in biocatalysis, bioassays, biosensing, and bioimaging, etc. Supplementary Information The online version contains supplementary material available at 10.1007/s00216-021-03597-w.

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Ultrasensitive chemiluminescence immunoassay with enhanced precision for the detection of cTnI amplified by acridinium ester-loaded microspheres and internally calibrated by magnetic fluorescent nanoparticles

Abstract: Hydrogel microspheres sensitive to temperature as new potential signal enhancers and magnetic fluorescent nanoparticles as internal standards were used to establish a new CLIA method for the accurate diagnosis of cTnI in the human body.

Cited by 85 publications

References 39 publications

Fluorescent Immunoassays for Detection and Quantification of Cardiac Troponin I: A Short Review

Fluorescent Immunoassays for Detection and Quantification of Cardiac Troponin I: A Short Review

Applications of Aptamer-Bound Nanomaterials in Cancer Therapy

Chemiluminescent magnetic nanoparticles with good catalytic activity and rapid separation capability and sensitive sensing for H2O2

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