Ultrasensitive and Simultaneous SERS Detection of Multiplex MicroRNA Using Fractal Gold Nanotags for Early Diagnosis and Prognosis of Hepatocellular Carcinoma

Wu, Jia‐Min; Zhou, Xia; Li, Ping; Lin, Xiaoling; Wang, Jinhua; Hu, Ziwei; Zhang, Pengcheng; Dong, Chen; Cai, Huaihong; Nießner, Reinhard; Haisch, Christoph; Sun, Ping‐Hua; Zheng, Yun; Jiang, Zhengjin; Zhou, Haibo

doi:10.1021/acs.analchem.1c00478

Cited by 70 publications

(33 citation statements)

References 67 publications

(83 reference statements)

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“…Wu et al have displayed the ultrasensitive and simultaneous SERS detection of multiple miRNAs using fractal gold-nanotags labeled by reporters. [22] Cheng et al have exhibited the SERS detection of free proteins in serum, showing that the simultaneous assay of free and total prostate-specific antigen provides better precision for the diagnosis of prostate cancer. [23] The latest research has shown that SERS is used to specifically characterize exosomes.…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive Exosome Detection for Early Diagnosis of Pancreatic Cancer Using Immunoassay Based on Hierarchical Surface‐Enhanced Raman Scattering Substrate

Chen

et al. 2022

Small Methods

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5 year survival rate of just 6% and a median survival of fewer than 6 months due to difficulties in the early diagnosis, PaC will be the second leading cause of cancer death by 2030. [3][4][5] Clinical diagnostic methods for PaC mainly include imaging and pathological biopsy. However, the available imaging diagnostic techniques, such as ultrasound (US), computed tomography (CT) scanning, and magnetic resonance imaging (MRI), have low diagnostic sensitivity and high requirements on operating skills. [2,[6][7][8] Normally, histopathological biopsy is a gold standard for disease diagnosis. [9] However, due to the deep position of the pancreas, biopsy will bring great harm to patients, and it needs to be punctured under the guidance of imaging and is not suitable for screening of the general population. [10,11] Moreover, the sensitivity and specificity of the widely used clinical screening biomarker carbohydrate antigen19-9 (CA19-9) are not high, and its serum level is significantly increased in inflammatory diseases of the pancreas and biliary tract, which leads its capacity for early diagnosis is not be evaluated. [12,13] Therefore, it is urgent and necessary to identify new and better biomarkers for the early diagnosis of PaC.Exosomes have emerged as potential biomarkers for pancreatic cancer (PaC). However, it is still challenging to get quantitative detection of exosomes with the specific surface receptors. In this study, a highly sensitive detection system is first constructed for the direct quantitation of specific exosomes in real samples using hierarchical surface-enhanced Raman scattering substrate (H-SERS substrate) and rapid enrichment strategy magnetic beads @ exosomes @ SERS detection probes (MEDP). It is found that the detection system (MEDP @ H-SERS substrate) could provide a 3.5 times higher SERS intensity compared with MEDP sandwich immunocomplex only. Moreover, LRG1-positive exosomes (LRG1-Exos) and GPC1-positive exosomes (GPC1-Exos) are chosen to distinguish PaC through exosome proteomics and database screening. The lower limit of detection (LOD) is 15 particles µL -1 using the MEDP @ H-SERS substrate. Significantly, the detection in clinical samples shows that the innovative combination of LRG1-Exos and GPC1-Exos could improve the diagnostic efficiency of PaC, with an area under the operating characteristic curve (AUC) of 0.95. Even for the early-stage PaC, the diagnostic accuracy is still high (AUC = 0.95). Collectively, the findings indicate that the MEDP @ H-SERS substrate has great potential for the early diagnosis of PaC.The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/smtd.202200154.

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Section: Introductionmentioning

confidence: 99%

Highly Sensitive Exosome Detection for Early Diagnosis of Pancreatic Cancer Using Immunoassay Based on Hierarchical Surface‐Enhanced Raman Scattering Substrate

Chen

et al. 2022

Small Methods

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“…The multiplex detection of three hepatocellular carcinoma-related miRNA (miRNA-122, miRNA-21, and miRNA-223) biomarkers using magnetically assisted sandwich-type SERS was recently demonstrated [ 68 ]. The detection is based on Raman dye-modified fractal AuNPs and Ag-coated magnetic NPs.…”

Section: In Vitro Cancer Biomarker Detectionmentioning

confidence: 99%

“… Schematic illustration of the use of a SERS-based method for the early diagnosis and prognosis of hepatocellular carcinoma. Reproduced with permission from [ 68 ]. Copyright 2021, American Chemical Society.…”

Section: Figurementioning

confidence: 99%

Multiplexed Liquid Biopsy and Tumor Imaging Using Surface-Enhanced Raman Scattering

Dell’Olio

2021

Biosensors

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The recent improvements in diagnosis enabled by advances in liquid biopsy and oncological imaging significantly better cancer care. Both these complementary approaches, which are used for early tumor detection, characterization, and monitoring, can benefit from applying techniques based on surface-enhanced Raman scattering (SERS). With a detection sensitivity at the single-molecule level, SERS spectroscopy is widely used in cell and molecular biology, and its capability for the in vitro detection of several types of cancer biomarkers is well established. In the last few years, several intriguing SERS applications have emerged, including in vivo imaging for tumor targeting and the monitoring of drug release. In this paper, selected recent developments and trends in SERS applications in the field of liquid biopsy and tumor imaging are critically reviewed, with a special emphasis on results that demonstrate the clinical utility of SERS.

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“…Surface-enhanced Raman scattering (SERS) is a developing analytic technology that can amplify greatly the Raman spectral signals of analytes absorbed on some special substrate materials. , Because of the advantages, including quick response, rich fingerprint information, high sensitivity, wide detection range, and simple pretreatment, SERS analysis shows great application potential in many fields such as biological tissue, , food safety, − environmental detection, , drug analysis, and other fields. − However, there is still a certain distance for this novel sensing technology to wide spread, due to the limitations of the SERS substrate. In general, most of the current SERS substrates suffer from the following problems: (1) it is difficult to take into account both high sensitivity and long-term stability. ,, Fresh Ag nanostructures usually show much better SERS activities than Au nanomaterials. ,,, (2) The current SERS substrates are either in solid state or liquid state. , Usually, solid-state SERS substrates are much more popular than liquid-state SERS substrates because they are easier to operate.…”

Section: Introductionmentioning

confidence: 99%

Hybridizing Silver Nanoparticles in Hydrogel for High-Performance Flexible SERS Chips

Chen

Zhang

Zhu

et al. 2022

ACS Appl. Mater. Interfaces

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An ideal surface-enhanced Raman scattering (SERS) substrate should have high sensitivity, long-term stability, excellent repeatability, and strong anti-interference. In the present work, single-layer carbon-based dot (CD)-capped Ag nanoparticle aggregates (a-AgNPs/CDs) with high SERS activity are synthesized and hybridized with a hydrogel to prepare novel hydrogel SERS chips. Benefiting from the unique properties of a-AgNPs/CDs and the hydrogel, the constructed hydrogel SERS chips show excellent performances. Taking crystal violet detection as an example, the hydrogel SERS chips show a detection limit of around 1 × 10–16 mol/L (high sensitivity), maintain above 96.40% of SERS activity even after 14 weeks of storage (long-term stability), and display point-to-point relative standard deviation (RSD) in one chip as low as 1.43% (outstanding repeatability) and RSD in different chips as low as 2.75% (excellent reproducibility). Furthermore, the self-extraction effect of the hydrogel enables the flexible hydrogel SERS chips to be used for analyzing various real samples including soybean milk, juices, and fruits without any complex pretreatment. For instance, the hydrogel SERS chips are able to detect trace thiram and 2-(4-thiazolyl)benzimidazole with the detection limits of 1 and 5 ppb in liquid samples, respectively, and of 1 and 2.5 ng/cm2 on the peel of fruits, respectively. The self-extraction functional flexible SERS chips offer a reliable and convenient platform for the quick detection and on-site monitoring of chemical contaminants.

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Ultrasensitive and Simultaneous SERS Detection of Multiplex MicroRNA Using Fractal Gold Nanotags for Early Diagnosis and Prognosis of Hepatocellular Carcinoma

Cited by 70 publications

References 67 publications

Highly Sensitive Exosome Detection for Early Diagnosis of Pancreatic Cancer Using Immunoassay Based on Hierarchical Surface‐Enhanced Raman Scattering Substrate

Highly Sensitive Exosome Detection for Early Diagnosis of Pancreatic Cancer Using Immunoassay Based on Hierarchical Surface‐Enhanced Raman Scattering Substrate

Multiplexed Liquid Biopsy and Tumor Imaging Using Surface-Enhanced Raman Scattering

Hybridizing Silver Nanoparticles in Hydrogel for High-Performance Flexible SERS Chips

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