Ultrapure water for liquid chromatography–mass spectrometry studies

Regnault, Cécilia; Kano, Ichiro; Darbouret, Daniel; Mabic, Stéphane

doi:10.1016/j.chroma.2003.10.043

Cited by 11 publications

(8 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Organic compounds present in the weaker solvent (Solvent A) are of particular concern as they tend to concentrate on the column during equilibration and elute during the gradient, giving rise to "ghost peaks" in a chromatogram [2]. Using the highest-purity water available for LC is always a prudent practice [3]. Improperly cleaned glassware is often associated with anomalous results in LC separations; soap residues have also been associated with ghost peaks in a chromatogram [4].…”

mentioning

confidence: 99%

Cyclic polyamide oligomers extracted from nylon 66 membrane filter disks as a source of contamination in liquid chromatography/mass spectrometry

Tran

Doucette

2006

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

Background interferences are perhaps an unavoidable part of analytical detection schemes, particularly when analyzing trace level samples or when using detectors with high levels of sensitivity. In liquid chromatography, mobile phase solvents are routinely filtered using membrane filter disks to trap particulates in hopes of minimizing contamination, providing improvements in data output and instrumental operation. In this study, we report that one such filter disk leads to a significant level of contamination in LC and LC/MS experiments. Extractable compounds from nylon membrane filters generate significant background signals in UV absorption chromatograms at 214 nm, and are also detected by electrospray ionization mass spectrometry, with nominal m/z values of 453 and 679. It is shown that rinsing the nylon membranes before their use can reduce, but will not eliminate, the extractable contaminants from the mobile phase. Through MS and tandem MS analysis, we have identified these contaminants as cyclic oligomers of polyamide 66. Based on these results, it is recommended that nylon membrane filters be avoided when conducting trace level analysis, particularly when conducting LC/MS experiments. (J Am Soc Mass Spectrom 2006, 17, 652-656)

show abstract

mentioning

confidence: 99%

Cyclic polyamide oligomers extracted from nylon 66 membrane filter disks as a source of contamination in liquid chromatography/mass spectrometry

Tran

Doucette

2006

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

show abstract

“…In gradient elution mode, during equilibration prior to the sample injection (with 100% of water in our study), impurities in the mobile phase are adsorbed/partitioned and concentrated at the head of the column. It was found that as the length of equilibration time increased, the amount of the impurity concentrated in the column increased 8, 9. During the gradient elution, as the organic modifier concentration in the mobile phase (acetonitrile in our study) is increased, these impurities are eluted according to their hydrophobicities, along with the analytes of interest.…”

Section: Resultsmentioning

confidence: 62%

“…Only a couple of studies carried out with LC/MS have some implications on mobile phase impurities. The benefits of the UV photooxidation process in water purification systems on the reduction of total organic carbons was shown using electrospray ionisation (ESI) LC/MS 9. Some workers10 observed that the change in source and grade of methanol can affect the ESI LC/MS signal and this effect was attributed to the differences in the concentrations of impurities in methanol.…”

mentioning

confidence: 99%

Effect of ionization suppression by trace impurities in mobile phase water on the accuracy of quantification by high‐performance liquid chromatography/mass spectrometry

Herath

Shaw

Cabot

et al. 2010

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

The high-performance liquid chromatography (HPLC) column is capable of enrichment/pre-concentration of trace impurities in the mobile phase during the column equilibration, prior to sample injection and elution. These impurities elute during gradient elution and result in significant chromatographic peaks. Three types of purified water were tested for their impurity levels, and hence their performances as mobile phase, in HPLC followed by total ion current (TIC) mode of MS. Two types of HPLC-grade water produced 3-4 significant peaks in solvent blanks while LC/MS-grade water produced no peaks (although peaks were produced by LC/MS-grade water also after a few days of standing). None of the three waters produced peaks in HPLC followed by UV-Vis detection. These peaks, if co-eluted with analyte, are capable of suppressing or enhancing the analyte signal in a MS detector. As it is not common practice to run solvent blanks in TIC mode, when quantification is commonly carried out using single ion monitoring (SIM) or single or multiple reaction monitoring (SRM or MRM), the effect of co-eluting impurities on the analyte signal and hence on the accuracy of the results is often unknown to the analyst. Running solvent blanks in TIC mode, regardless of the MS mode used for quantification, is essential in order to detect this problem and to take subsequent precautions.

show abstract

“…The flow rate and run time were 2.0 ml/min and 30 min, respectively. The HPLC analysis was performed at detection wavelengths of 210 and 254 nm [ 33 ]. In addition, HPLC analysis (at 254 nm) of lab water (18.2 MΩ × cm at 25°C) stored for three days at room temperature (15–30°C) in a soda lime glass (type III) (Wheaton, USA) container tightly covered with plastic stoppers was conducted.…”

Section: Methodsmentioning

confidence: 99%

Quality-by-Design Principles Applied to the Establishment of a Pharmaceutical Quality Control Laboratory in a Resource-Limited Setting: The Lab Water

Suleman

Belew

Kebebe

et al. 2022

International Journal of Analytical Chemistry

View full text Add to dashboard Cite

Quality-by-design (QbD) is defined as a systematic approach to design and develop a product/service based on sound science and quality risk management. It is already frequently applied in the pharmaceutical industry mainly in the development of pharmaceutical products and analytical methods but is not well established in the setup of facilities like quality control (QC) laboratory (lab). Therefore, lab QbD (lQbD) concept is introduced considering lab water purification system as an example. The water purification system comprising distillation unit coupled with Nanopure Analytical Ultrapure Water System combined with a 0.2-micron filter was established in Jimma University Laboratory of Drug Quality (JuLaDQ). The consistent capability of the established water purification system was evaluated through routine monitoring of the critical quality parameters (i.e., physicochemical, HPLC-DAD chromatogram total peak area, and resistivity) of freshly prepared lab water for a period of one year. In addition, quality of different grade water (tap water, distilled water (before and/or after cleaning distillation unit), and fresh ultrapure water (18.2 MΩ × cm at 25°C)) used in JuLaDQ was evaluated. The results of routine analysis of water quality revealed that HPLC global peak area at 210 and 254 nm could serve as one of the discriminatory control strategies to evaluate the capability of water purification system to produce the desired quality of lab water; and thus, we proposed a specification limit of 5,000 mAU∗s and 5,500 mAU∗s for global peak area at 254 and 210 nm, respectively, as system suitability parameter.

show abstract

Ultrapure water for liquid chromatography–mass spectrometry studies

Cited by 11 publications

References 11 publications

Cyclic polyamide oligomers extracted from nylon 66 membrane filter disks as a source of contamination in liquid chromatography/mass spectrometry

Cyclic polyamide oligomers extracted from nylon 66 membrane filter disks as a source of contamination in liquid chromatography/mass spectrometry

Effect of ionization suppression by trace impurities in mobile phase water on the accuracy of quantification by high‐performance liquid chromatography/mass spectrometry

Quality-by-Design Principles Applied to the Establishment of a Pharmaceutical Quality Control Laboratory in a Resource-Limited Setting: The Lab Water

Contact Info

Product

Resources

About