2010
DOI: 10.1002/rcm.4549
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Effect of ionization suppression by trace impurities in mobile phase water on the accuracy of quantification by high‐performance liquid chromatography/mass spectrometry

Abstract: The high-performance liquid chromatography (HPLC) column is capable of enrichment/pre-concentration of trace impurities in the mobile phase during the column equilibration, prior to sample injection and elution. These impurities elute during gradient elution and result in significant chromatographic peaks. Three types of purified water were tested for their impurity levels, and hence their performances as mobile phase, in HPLC followed by total ion current (TIC) mode of MS. Two types of HPLC-grade water produc… Show more

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Cited by 11 publications
(8 citation statements)
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“…Liquid chromatography coupled to mass spectrometry (LC/MS) is becoming ever more important in the identification and characterization of unknowns in complex systems . Soft ionization techniques such as electrospray ionization (ESI), as well as the wide variety of separation phases available, make LC/MS an appealing option for separations containing molecules that are able to accommodate a charge.…”
Section: Introductionmentioning
confidence: 99%
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“…Liquid chromatography coupled to mass spectrometry (LC/MS) is becoming ever more important in the identification and characterization of unknowns in complex systems . Soft ionization techniques such as electrospray ionization (ESI), as well as the wide variety of separation phases available, make LC/MS an appealing option for separations containing molecules that are able to accommodate a charge.…”
Section: Introductionmentioning
confidence: 99%
“…Impurities may include plasticizers, polymers, human‐derived proteins, and others, and sources may include solvents, glassware, storage vials, and reagents used to prepare samples . Depending on the nature of the impurity, it may be concentrated during gradient separations and elute as a peak . Some impurity peaks may go unnoticed in LC/MS separations, especially when targeted methods are used.…”
Section: Introductionmentioning
confidence: 99%
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“…[21,22] Even for pure water gradient elution the presence of this type of ions has been shown. [23] Second type ions, in the case of sample analysis ionisable matrix compounds may be present. In addition to yielding ions, the matrix compounds may either cause ionisation suppression/ enhancement of the analyte signal or have no influence on analyte ionisation.…”
mentioning
confidence: 99%