Ultramicroanalysis: X-Ray Spectrometry by Electron Probe Excitation

Lechène, C.; Warner, R.R.

doi:10.1146/annurev.bb.06.060177.000421

Cited by 55 publications

(18 citation statements)

References 5 publications

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“…The techniques of sample preparation and electron probe microanalysis of picolitre samples have been previously described (see Lechene & Warner, 1977, for a review; Borland et al 1976Borland et al , 1977a. Briefly, the unknown solutions and a series of standard solutions were placed with calibrated picolitre volumetric pipettes (20-80 pl.)…”

Section: Sample Preparation and Electron Probe Microanalyaiamentioning

confidence: 99%

Ouabain‐sensitive fluid accumulation and ion transport by rabbit blastocysts.

Biggers¹,

Borland²,

Lechène³

1978

The Journal of Physiology

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SUMMARY1. By incubating 6-day post-coitum (p.c.) blastocysts in medium supplemented with either 20 mm-sucrose or 10 mM-KCl it was possible to show that the trophectoderm of the blastocyst prevents the concentration of K in the blastocoele fluid rising to external concentrations.2. The concentrations of K in the blastocoele fluid are maintained predominantly by leakage of K from the trophoblast cells into the blastocoele and by ouabainsensitive transport of K into the trophoblast cells from the blastocoele fluid.3. Exposure of blastocysts to ouabain on the juxtacoelic, but not abcoelic, surface of the trophectoderm may inhibit blastocoele fluid accumulation.

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Section: Sample Preparation and Electron Probe Microanalyaiamentioning

confidence: 99%

Ouabain‐sensitive fluid accumulation and ion transport by rabbit blastocysts.

Biggers¹,

Borland²,

Lechène³

1978

The Journal of Physiology

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“…Numerous techniques have been developed for determination of subcellular distribution of ions by X-ray microanalysis [5,6]. However, in the present study we are dealing with extracellularly located particles of supracellular magnitude.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, the recently developed technique of X-ray micro analysis provides a simultaneous determination of elemen tal composition of a selected area, performed under visual control [4][5][6]. For this reason X-ray dispersive micro analysis is potentially an important tool in the examination of the chemical composition of calcificates.…”

mentioning

confidence: 99%

X-Ray Microanalysis of Uremic Nephrocalcinosis

Goligorsky

Chaimovitz

Rapoport

et al. 1983

Nephron

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The nature of uremic nephrocalcinosis was studied by means of X-ray microanalysis in 5/6 nephrectomized rats. The data show that calcified deposits in the renal parenchyma of uremic rats contained substantial amounts of calcium, magnesium, aluminum and silicon. Since calcificates did not reveal a uniform composition they were arbitrarily divided into 3 categories: ‘high-calcium’ with Mg: Al: Si: Ca molar ratios 7:3:8:37; ‘low-calcium’ particles were characterized by Fe:Mg:Al:Ca:Si molar ratios 7:3:5:6:13; while ‘intermediate-calcium’ particles showed Al: Mg: Si: Ca molar ratios equivalent to 2:4:6:7. The variability of calcified deposits in the renal parenchyma of 5/6 nephrectomized rats might be due to different sources, different environmental conditions along the nephron and different stages of calcification process. It is suggested that aluminum and silicon content of deposits may be of value in further characterization of uremic nephrocalcinosis.

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“…Three separate groups of workers showed that the plateletdense bodies are indeed storehouses of cal cium and phosphorus [5,12,18]. Martin et al [18] showed that these elements are in a stable form.…”

Section: Platelet X-ray Microprobe Analyses Reported In the Literaturementioning

confidence: 99%

“…As a rule local concentrations above 50 ppm are detectable, but it should be remembered that no distinction is made be tween the various forms of each element. Quick-freezing and ultracryotomy [5,6] is most likely to preserve natural conditions, but the technique is difficult and not widely used. Compromise procedures such as freeze substitution or vacuum embedding and sec tioning have all been employed.…”

Section: Introductionmentioning

confidence: 99%

X-Ray Microprobe Analysis of Platelets

Yarom¹

1983

Pathophysiol Haemos Thromb

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Platelets are well suited to X-ray microanalysis as there is no need for chemical fixation or sectioning, and the concentrations of calcium and phosphorus are above 10^-3. The principles of the technique, the methods of specimen preparation, instrumental conditions during analysis and ways of quantitation are described. This is followed by a review of published reports and a brief summary of the author’s own work in the field.

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Ultramicroanalysis: X-Ray Spectrometry by Electron Probe Excitation

Cited by 55 publications

References 5 publications

Ouabain‐sensitive fluid accumulation and ion transport by rabbit blastocysts.

Ouabain‐sensitive fluid accumulation and ion transport by rabbit blastocysts.

X-Ray Microanalysis of Uremic Nephrocalcinosis

X-Ray Microprobe Analysis of Platelets

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