Ouabain‐sensitive fluid accumulation and ion transport by rabbit blastocysts.

Biggers, John D.; Borland, Raymond M.; Lechène, C.

doi:10.1113/jphysiol.1978.sp012386

Cited by 35 publications

(18 citation statements)

References 19 publications

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“…Formation of tight junctions and adherens junctions including binding of the catenin-cadherin complex to actin cytoskeleton is necessary for polarization of blastomeres in morula stage embryos and is a prerequisite for formation of the blastocyst [25]. The polarized trophectoderm can engage in active transport of water and select nutrients into the blastocoel [26,27].…”

Section: The Uterine Lumenal Microenvironment Derives From the Secretmentioning

confidence: 99%

Select Nutrients in the Uterine Lumen of Sheep and Pigs Affect Conceptus Development

Bazer

Kim

et al. 2012

J. Reprod. Dev.

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Abstract. Interferon tau (IFNT) is the pregnancy recognition signal from ruminant conceptuses. IFNT also acts with P4 to induce expression of genes for transport of nutrients, such as glucose (Gluc) and arginine (Arg) into the uterine lumen to activate mechanistic mammalian target of rapamycin (MTOR) cell signaling that stimulates proliferation, migration, gene transcription and mRNA translation by conceptus trophectoderm (Tr). In ewes, Arg and Gluc increase significantly in the uterine lumen between Days 10 and 15 of pregnancy due to increased expression of transporters for Gluc (SLC2A1 and SLC5A1) and Arg (SLC7A2B) by uterine epithelia. Arg and Gluc stimulate proliferation, migration and mRNA translation by Tr. Arg increases expression of GTP cyclohydrolase 1 (GCH1) and IFNT mRNAs while Arg and Gluc increase ornithine decarboxylase, nitric oxide synthase 2, and GCH1 mRNAs and proteins by Tr cells. GCH1 is required for synthesis of tetrahydrobiopterin, an essential cofactor for all NOS isoforms. Arg is metabolized to nitric oxide and polyamines that increase proliferation and migration of Tr cells. In pigs, Gluc, Arg, leucine (Leu) and glutamine (Gln) increase in the uterine lumen between Days 12 and 15 of pregnancy due to enhanced expression of transporters for Gluc and amino acids. Transporters for Gluc in porcine uterine LE (SLC2A1) and conceptus trophectoderm (SLC2A2) are abundant. Transporters for glutamate and neutral (SLC1A1, SLC1A4) and cationic (SLC7A1, SLC7A2, SLC7A7, SLC7A9) amino acids are expressed in uterine LE and SLC7A3 mRNA is expressed in conceptus Tr. Arg and Leu increase MTOR cell signaling and proliferation of pig Tr, as do Gluc and fructose. Azaserine, an inhibitor of hexosamine biosynthesis, inhibits effects of Gluc and fructose. Thus, select nutrients in the uterine lumen affect gene transcription and mRNA translation to affect conceptus development. Key words: Amino acids, Fructose, Glucose, Uterus conceptus development (J. Reprod. Dev. 58: [180][181][182][183][184][185][186][187][188] 2012) E stimates of embryonic death loss in mammals range from 20 to 40 percent and two-thirds of these losses occur during the peri-implantation period of pregnancy [1]. Establishment and maintenance of pregnancy requires appropriate development of the conceptus (embryo/fetus and its extra-embryonic membranes) for pregnancy recognition signaling to ensure maintenance of a functional corpus luteum (CL) to secrete progesterone (P4) that is required for an intrauterine environment that supports implantation, placentation and uterine functions essential for successful outcomes of pregnancy [2]. Interactions among the conceptus and uterine cell types, especially luminal (LE), superficial glandular (sGE) and glandular (GE) epithelia and stromal cells (SC), are essential for conceptus development, signaling for maternal recognition of pregnancy, uterine blood flow, water and electrolyte movement, transport of sugars and amino acids into the uterine lumen, as well as secretion of proteins by uterine epithelia...

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Section: The Uterine Lumenal Microenvironment Derives From the Secretmentioning

confidence: 99%

Select Nutrients in the Uterine Lumen of Sheep and Pigs Affect Conceptus Development

Bazer

Kim

et al. 2012

J. Reprod. Dev.

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“…The appearance of the Chloride channels in the mouse conceptus voltage-inactivated Cl K conductance was associated with the emergence of this trophoblast lineage (Johnson 1986(Johnson , 2009, suggesting a role for this conductance in the vectorial movement of electrolytes and water into the blastocoel cavity. Expansion of the blastocoel cavity in the mouse blastocyst requires extracellular Cl K , is dependent on both paracellular and transcellular movement of Cl K across the TE (Manejwala et al 1989, Brison & Leese 1993, Zhao et al 1997, and is driven by the basolaterally localized Na 1977, Biggers et al 1978, Watson & Kidder 1988. In this study, DIDS did not completely prevent blastocyst expansion, as observed previously (Zhao et al 1997), an observation that suggested a role for other pathways for Cl K movement such as the paracellular pathway or the DIDS-insensitive, voltage-inactivated current described here.…”

Section: Discussionmentioning

confidence: 99%

Changing expression of chloride channels during preimplantation mouse development

et al. 2013

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Plasma membrane chloride channels (ClCs) play important roles in a broad range of cellular processes including cell volume regulation, proliferation, and transepithelial transport, all of which are critical during preimplantation embryonic development. In this study, the molecular and functional expression of voltage-gated ClCs was analyzed throughout preimplantation development of the mouse conceptus. mRNA transcripts for all Clcn genes were detected. Only Clcn1 mRNA showed differential expression in the blastocyst, being detected in the trophectoderm but not in the inner cell mass. CLCN3 protein was detected at low levels in the cytoplasm and plasma membrane in 4-cell embryos and was localized to the apical plasma membrane of the trophoblasts in the blastocyst. Whole-cell patchclamp recordings demonstrated the presence of a DIDS-sensitive, outwardly rectifying Cl K current throughout development, with this conductance being large at the 1-cell, morula and blastocyst stages. A second DIDS-insensitive Cl K current, which was inactivated by membrane depolarization, was present in cells differentiating into the trophoblast lineage and during blastocyst expansion. Inhibition of the DIDS-sensitive current and the DIDS-insensitive current, with 9-AC, prevented blastocyst expansion.

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“…For example, net transtrophectodermalNa+ influx increases 10-fold during this period (Borland etal,\ 976). Ouabain, a specific inhibitor of Na+/K + ATPase, has been shown to inhibit both the accumulation of blastocyst fluid (Biggers et al, 1978) and > 50% of net trans-trophectodermal Na+ transport in preimplantation rabbit blastocysts (Benos, 1981b).…”

Section: Free Intracellular Methionine Contentmentioning

confidence: 99%

“…Mechanisms of active transepithelial solute transport have been reported to mediate this process of blastocoele expansion (see Benos & Biggers, 1981;Benos et al, 1985, for reviews). Specifically, there is increasing evidence that the ubiquitous membrane-bound enzyme Na + /K+ ATPase (EC 3.6.1.3) mediates transtrophectodermal fluid transport in the preimplantation mammalian blastocyst (Biggers et al, 1978).…”

Section: Introductionmentioning

confidence: 99%