Ultrahigh field MRI determination of water diffusion rates in ex vivo human lenses of different age

Stahnke, Thomas; Lindner, Tobias; Guthoff, Rudolf; Stachs, Oliver; Wree, Andreas; Langner, Sönke; Niendorf, Thoralf; Grabow, Niels; Glass, Änne; Beller, Ebba; Polei, Stefan

doi:10.21037/qims-20-1124

Cited by 2 publications

(1 citation statement)

References 60 publications

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“…Different layers of the lens, like the epithelium, lens capsule, cortex, or within the nucleus, are identifiable with micro-CT ( Figures 2B,3,4,6A ) ( 15 ). Micro-CT scans of moist lenses allow for more detailed imaging than MRI ( 11 , 33 ), while the risk of artificial damages is reduced compared to histological methods ( 11 ). Within the lens nucleus, IPI contrasting enabled further differentiation ( Figure 3 ) which resembles the results of Scheimpflug photography ( 34 ).…”

Section: Discussionmentioning

confidence: 99%

Micro-CT in ophthalmology: ex vivo preparation and contrasting methods for detailed 3D-visualization of eye anatomy with special emphasis on critical point drying

Runge¹,

Stahnke²,

Guthoff³

et al. 2022

Quant Imaging Med Surg

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Background: Micro-computed tomography (micro-CT) provides detailed 3-dimensional (3D) visualization of anatomical structures and encourages morphological reinvestigation of organs with delicate features. The low radiodensity of soft tissues necessitates preceding sample preparation to conduct X-ray imaging with decent contrast between different tissues. In this study, we demonstrate the preparation with three radiopaque agents in combination with elimination of liquids by critical point drying (CPD) introduced for ocular samples.Methods: Enucleated porcine eyes were prepared with ethanolic iodine (EI), aqueous iodine-potassium iodide, or ethanolic phosphotungstic acid (EPTA). Micro-CT scans of the samples were conducted in a moist environment with an isotropic resolution of 9.2-12.5 µm voxel size. Subsequently, samples were chemically dehydrated and critical point (CP) dried to conduct a second scan in a dry environment with a resolution up to 4.7-5.4 µm in voxel size. The visualization effects were qualitatively and semi-quantitatively evaluated with regard to the generated contrast between different ocular tissues.Results: All three contrast agents accumulated well in most of the investigated ocular tissues and lead to an increased X-ray attenuation which allowed for differentiated visualization of ocular structures. Problematic agent penetration into the lens was obvious for iodine-potassium iodide and EPTA. Artificial damages of the lens and thickness reduction for the cornea and sclera due to CPD were noticed. The effects of the different contrasting treatments are described and compared with regard to the effects of CPD. Exclusively CP dried samples that were not treated with contrast agents could also be visualized excellently with a good distinction of different ocular structures from each other.Conclusions: All ocular structures can be visualized by micro-CT. To contrast moist samples, the best results were achieved with iodine potassium iodide (IPI). CPD improved the scan quality in all cases. Even without pretreatment with contrasting agents, the CP dried samples showed a contrast similar to the IPI treated samples.

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Section: Discussionmentioning

confidence: 99%

Micro-CT in ophthalmology: ex vivo preparation and contrasting methods for detailed 3D-visualization of eye anatomy with special emphasis on critical point drying

Runge¹,

Stahnke²,

Guthoff³

et al. 2022

Quant Imaging Med Surg

Self Cite

View full text Add to dashboard Cite

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Erratum to ultrahigh field MRI determination of water diffusion rates in ex vivo human lenses of different age

2021

Quant Imaging Med Surg

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The article "Ultrahigh field MRI determination of water diffusion rates in ex vivo human lenses of different age" (Quant Imaging Med Surg 2021;11:3029-41), written by Stahnke et al. (1), was published with some errors. This article that appeared on page 3032, unfortunately contained a mistake in Figure 2A. The corrected version of Figure 2A is presented here (Figure 2A). ErratumErratum to ultrahigh field MRI determination of water diffusion rates in ex vivo human lenses of different age

show abstract

Ultrahigh field MRI determination of water diffusion rates in ex vivo human lenses of different age

Cited by 2 publications

References 60 publications

Micro-CT in ophthalmology: ex vivo preparation and contrasting methods for detailed 3D-visualization of eye anatomy with special emphasis on critical point drying

Micro-CT in ophthalmology: ex vivo preparation and contrasting methods for detailed 3D-visualization of eye anatomy with special emphasis on critical point drying

Erratum to ultrahigh field MRI determination of water diffusion rates in ex vivo human lenses of different age

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