Ultrafiltration LC-ESI-MSn screening of MMP-2 inhibitors from selected Chinese medicinal herbs Smilax glabra Roxb., Smilax china L. and Saposhnikovia divaricata (Turcz.) Schischk as potential functional food ingredients

Li, Li; Bo, Li; Zhang, Huirong; Zhao, Anqi; Han, Binhua; Liu, Chunming; Tsao, Rong

doi:10.1016/j.jff.2015.03.038

Cited by 21 publications

(23 citation statements)

References 27 publications

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“…As a result, 4 SD phytochemical studies only [4][5][6][7] adhered to the scientific rigors that ensure the integrity in validation and robustness in quantitative outcomes. Among 9 phytochemistry papers with in vitro studies [8][9][10][11][12][13][14][15][16] , 5 [11,[13][14][15][16] were selected based on their relevance, but the phytochemistry component did not adhere to the selection criteria. A total of 10 in vitro studies only were included in this review [17][18][19][20][21][22][23][24][25][26] .…”

Section: Resultsmentioning

confidence: 99%

Saposhnikoviae divaricata : a phytochemical, pharmacological, and pharmacokinetic review

Kreiner

Pang

Lenon

et al. 2017

Chinese Journal of Natural Medicines

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Saposhnikoviae divaricata (Turcz.) Schischk (SD) is a traditional Chinese herb commonly used to treat clinical conditions such as rheumatism and allergic rhinitis. This review article evaluates a collection of works on in vitro and biochemical studies of SD. The discourse on the diverse class of chromones and coumarins in SD offers an insight to the pharmacological effects of these bioactive constituents as anti-inflammatory, analgesic, immunoregulatory, antioxidative, and anti-proliferative agents. It is highlighted that there is a structural relationship between the constituents and bioactive activities, which in effect provides a valid reasoning and reaffirm the use of SD in the treatment of the pathologies in Chinese medicine.

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Section: Resultsmentioning

confidence: 99%

Saposhnikoviae divaricata : a phytochemical, pharmacological, and pharmacokinetic review

Kreiner

Pang

Lenon

et al. 2017

Chinese Journal of Natural Medicines

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“…UF‐MS assay is an affinity‐based method, in which active compounds bind to MPO and are retained by the UF membrane, while unbound compounds are filtered out. The ligand‐receptor complex is disrupted by the addition of methanol, and the released ligands are then identified by LC‐MS analysis .…”

Section: Resultsmentioning

confidence: 99%

A novel strategy for evaluation of natural products acting on the myeloperoxidase/hypochlorous acid system by combining high‐performance liquid chromatography‐photodiode array detection‐chemiluminescence and ultrafiltration‐mass spectrometry techniques

Wang

Fang

et al. 2018

J of Separation Science

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Myeloperoxidase and its product hypochlorous acid are endogenous substances that are involved in the pathogenesis of many inflammatory diseases. In the present study, a novel evaluation strategy has been developed to screen for myeloperoxidase inhibitors and hypochlorous acid scavengers in natural products. The evaluation strategy uses two methods: a multi-hyphenated high-performance liquid chromatography-photodiode array detection-chemiluminescence online method, and an ultrafiltration-high-performance liquid chromatography-mass spectrometry method. Both were used for screening hypochlorous acid scavengers and myeloperoxidase inhibitors. After optimization of operating conditions, including pH of mobile phase and concentrations of myeloperoxidase, hypochlorous acid and luminol, the evaluation strategy was applied to Gardenia jasminoides Ellis (Rubiaceae) extract. Gardenia jasminoides Ellis (Rubiaceae) extract was found to both scavenge hypochlorous acid and inhibit myeloperoxidase. These different bioactivities originate from iridoid glycosides and quinic acid derivatives (25 hypochlorous acid scavengers) and crocetin derivatives (4 myeloperoxidase inhibitors). In a mouse model of acute lung injury, pulmonary histopathology showed that different constituents of Gardenia jasminoides Ellis (Rubiaceae) extract could also attenuate lung injury. Although the screening strategy has two arms, it is still simple, rapid, and effective. The strategy can also potentially be used to discriminate different activities of different constituents contained in the same natural product.

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“…The enhancement factor (Table 2) was introduced to further understand the relative binding strength of each compound [17, 20]. This factor is proportional to the LC peak area of the ligand bound to the target enzyme; thus the higher factor means more bound compound.…”

Section: Resultsmentioning

confidence: 99%

“…The enhancement factor (%) = ( A 1 / A 2 ) × 100% was used for calculating the binding of a ligand to PDEI, where A 1 and A 2 are the amounts of compound bound to PDEI and the total amount of compound in incubation, respectively [17, 20]. …”

Section: Methodsmentioning

confidence: 99%

“…Combined with LC–MS which has become a powerful technique for simultaneous separation and identification of components, ultrafiltration LC–MS has become the state-of-the-art technique in screening and identifying active constituents in complex extracts, owing to the capability of separation of ligand-protein complexes from unbound compounds by ultrafiltration and the capability of identification of the ligands by LC–MS [17–19]. …”

Section: Introductionmentioning

confidence: 99%

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Rapid Screening of Potential Phosphodiesterase Inhibitors from the Roots of Ilex pubescens Hook. et Arn. Using a Combination of Ultrafiltration and LC–MS

Liu

Lin

Chen

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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The cyclic nucleotide phosphodiesterase (PDE) plays an important role in regulating the levels of second messenger molecules cAMP and cGMP. Various PDE inhibitors have been successfully developed into drugs for targeted diseases. In addition, PDE inhibitors can also be found in different foods and natural medicines. In this study, ultrafiltration liquid chromatography–diode-array detector–electrospray ionization–ion-trap–time-of-flight–mass spectrometry (ultrafiltration LC–DAD–ESI–IT–TOF–MS) was applied to screen PDE inhibitors from the roots of Ilex pubescens Hook. et Arn. As a result, 11 major compounds were identified in I. pubescens roots, with nine compounds as potential PDE inhibitors, among which five were further confirmed to be active against PDEI and PDE5A dose-dependently in vitro, with ilexsaponin A1 and ilexsaponin B2 being the strongest. HPLC quantification of these bioactive compounds suggested that they are major components in the plant. The results demonstrate that ultrafiltration LC–DAD–ESI–IT–TOF–MS is an efficient method for rapid screening of PDE inhibitors from natural medicines.

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Ultrafiltration LC-ESI-MSn screening of MMP-2 inhibitors from selected Chinese medicinal herbs Smilax glabra Roxb., Smilax china L. and Saposhnikovia divaricata (Turcz.) Schischk as potential functional food ingredients

Cited by 21 publications

References 27 publications

Saposhnikoviae divaricata : a phytochemical, pharmacological, and pharmacokinetic review

Saposhnikoviae divaricata : a phytochemical, pharmacological, and pharmacokinetic review

A novel strategy for evaluation of natural products acting on the myeloperoxidase/hypochlorous acid system by combining high‐performance liquid chromatography‐photodiode array detection‐chemiluminescence and ultrafiltration‐mass spectrometry techniques

Rapid Screening of Potential Phosphodiesterase Inhibitors from the Roots of Ilex pubescens Hook. et Arn. Using a Combination of Ultrafiltration and LC–MS

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