Ultra-trace quantification method for chlordecone in human fluids and tissues

Bichon, Emmanuelle; Guiffard, Ingrid; Vénisseau, Anaïs; Marchand, Philippe; Antignac, Jean‐Philippe; Bizec, Bruno Le

doi:10.1016/j.chroma.2015.07.013

Cited by 27 publications

(15 citation statements)

References 22 publications

(40 reference statements)

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“…Methanol addition proved later to be a good solvent to mix with water to recover compounds from soil particles. As first described by Harless et al, [38] and observed by others [24,39], CLD actually exists as a gem-diol (hydrate) in water or a hemiacetal in methanol and can also PLOS ONE form various adducts in the presence of compounds often used in LC eluents, such as acetate and formate. In this study chromatographic separation, ionization and signal intensity were maximized when ammonium acetate was included in the water and methanol eluents.…”

Section: Development Of An Lc/ms Methods For Chlordecone and Metabolitesmentioning

confidence: 86%

Evidence for extensive anaerobic dechlorination and transformation of the pesticide chlordecone (C10Cl10O) by indigenous microbes in microcosms from Guadeloupe soil

et al. 2020

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The historic use of chlordecone (C 10 Cl 10 O) as a pesticide to control banana weevil infestations has resulted in pollution of large land areas in the French West Indies. Although currently banned, chlordecone persists because it adsorbs strongly to soil and its complex bishomocubane structure is stable, particularly under aerobic conditions. Abiotic chemical transformation catalyzed by reduced vitamin B 12 has been shown to break down chlordecone by opening the cage structure to produce C9 polychloroindenes. More recently these C9 polychloroindenes were also observed as products of anaerobic microbiological transformation. To investigate the anaerobic biotransformation of chlordecone by microbes native to the French West Indies, microcosms were constructed anaerobically from chlordecone impacted Guadeloupe soil and sludge to mimic natural attenuation and eletron donor-stimulated reductive dechlorination. Original microcosms and transfers were incubated over a period of 8 years, during which they were repeatedly amended with chlordecone and electron donor (ethanol and acetone). Using LC-MS, chlordecone and degradation products were detected in all the biologically active microcosms. Observed products included monohydro-, dihydro-and trihydrochlordecone derivatives (C 10 Cl 10-n O 2 H n ; n = 1,2,3), as well as "open cage" C9 polychloroindene compounds (C 9 Cl 5-n H 3+n n = 0,1,2) and C10 carboxylated polychloroindene derivatives (C 10 Cl 4-n O 2 H 4+n , n = 0-3). Products with as many as 9 chlorine atoms removed were detected. These products were not observed in sterile (poisoned) microcosms. Chlordecone concentrations decreased in active microcosms as concentrations of products increased, indicating that anaerobic dechlorination processes have occurred. The data enabled a crude estimation of partitioning coefficients between soil and water, showing that carboxylated intermediates sorb poorly and as a consequence may be flushed away, while polychlorinated indenes sorb strongly to soil. Microbial community analysis in microcosms revealed enrichment of anaerobic fermenting and acetogenic microbes possibly involved in anaerobic chlordecone biotransformation. It thus should be possible to

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Section: Development Of An Lc/ms Methods For Chlordecone and Metabolitesmentioning

confidence: 86%

Evidence for extensive anaerobic dechlorination and transformation of the pesticide chlordecone (C10Cl10O) by indigenous microbes in microcosms from Guadeloupe soil

et al. 2020

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“…Today, GC is still used but coupled with mass spectrometry for its higher specificity, especially at low concentrations [10]. However, more recent studies use liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) preceded, or not, by an isotopic dilution for quantifying CLD in food, tissues, or biological fluids leading to an LOQ of only a few µg kg −1 [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Development and Application of Molecularly Imprinted Polymers for the Selective Extraction of Chlordecone from Bovine Serum

et al. 2021

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The widespread use of chlordecone (CLD), an organochlorine pesticide, until the 1990s to protect banana crops in the French West Indies led to significant pollution of water and soil and, subsequently, of bovine intended for human consumption. Carcasses are submitted to official controls based on perirenal fat CLD determination. In order to allow for pre-slaughter controls, a selective analytical method based on a molecularly imprinted polymer (MIP) associated to the LC/MS-MS method was developed to determine the level of CLD in bovine serum that can be collected before slaughter. Different synthesis conditions were therefore assayed by varying the nature of the monomer and of the porogen, and the most promising MIP in terms of selective retention for CLD (extraction recovery close to 100%) was completely characterized by solid-phase extraction (repeatability of the extraction procedure, of the synthesis, and of the cartridge filling) in pure medium. The capacity of the MIP was determined at 0.13 µmol g−1 of MIP. After application of a spiked bovine serum sample on the MIP, the selective retention was maintained (87 and 21%, respectively, on the MIP and on the corresponding non-imprinted polymer). Moreover, extraction on the MIP led to a cleaner extract compared to those issued from a conventional C18 sorbent.

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“…The assay time points are given in Table 1. Four concurrent quantifying methods were performed to analyze BPS/ MEHP, BDE-47, pp 0 DDE=HCB=bHCH, and PFOA/PFOS, respectively, using liquid chromatography-tandem mass spectrometry (LC-MS/MS) measurement for perfluorinated compounds (PFAS; Cariou et al 2015), GC-HRMS measurement for polybromodiphenylether (PBDE) compounds (BDE-47; Antignac et al 2009) and gas chromatography-tandem mass spectrometry (GC-MS/MS) measurement, on one hand for organochlorine pesticides (OC; Bichon et al 2015) and on the other hand, bisphenols and phthalates with detailed conditions described elsewhere (Deceuninck et al 2015(Deceuninck et al , 2019. Serum concentrations of BPS (total and unconjugated), MEHP, PFOA, and PFOS were also evaluated in the samples collected from control rabbits on the day of drug administrations and 14 d later.…”

Section: Sample Analysismentioning

confidence: 99%

Use of Mixture Dosing and Nonlinear Mixed Effect Modeling of Eight Environmental Contaminants in Rabbits to Improve Extrapolation Value of Toxicokinetic Data

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Picard‐Hagen

et al. 2021

Environ Health Perspect

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Ultra-trace quantification method for chlordecone in human fluids and tissues

Cited by 27 publications

References 22 publications

Evidence for extensive anaerobic dechlorination and transformation of the pesticide chlordecone (C10Cl10O) by indigenous microbes in microcosms from Guadeloupe soil

Evidence for extensive anaerobic dechlorination and transformation of the pesticide chlordecone (C10Cl10O) by indigenous microbes in microcosms from Guadeloupe soil

Development and Application of Molecularly Imprinted Polymers for the Selective Extraction of Chlordecone from Bovine Serum

Use of Mixture Dosing and Nonlinear Mixed Effect Modeling of Eight Environmental Contaminants in Rabbits to Improve Extrapolation Value of Toxicokinetic Data

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